Construct: sgRNA BRDN0001145944
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAATCCCGTCATATGACTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NLK (51701)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76730
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
28168503 |
+ |
NLK |
NNNATCCCGTCATATGACTC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
9370518 |
- |
SEMA5A |
NNNATTCCTTCATATGACTC |
NGG |
2 |
0.4952 |
Tier II |
| 3 |
NC_000006.12 |
6 |
127735985 |
+ |
THEMIS |
NNNATCCAGTCATATGAATC |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000006.12 |
6 |
12957182 |
- |
PHACTR1 |
NNNATCCCGTCACCTGACTC |
NGG |
2 |
0.1692 |
Tier II |
| 5 |
NC_000018.10 |
18 |
54285196 |
+ |
POLI |
NNNCTCCCGTGATATGACTC |
NGG |
2 |
0.0882 |
Tier II |
| 6 |
NC_000001.11 |
1 |
2295848 |
+ |
SKI |
NNNATCCCGTCATTTGTCTC |
NGG |
2 |
0.0711 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
78587024 |
- |
Nlk |
NNNATCCCGTCATATGACTC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000079.6 |
13 |
93881581 |
- |
Arsb |
NNNATGCCATCATATGACTC |
NGG |
2 |
0.3214 |
Tier II |
| 3 |
NC_000076.6 |
10 |
121413987 |
- |
Rassf3 |
NNNATCCAGTCAGATGACTC |
NGG |
2 |
0.1696 |
Tier II |
Other clones with same target sequence:
(none)
