Construct: sgRNA BRDN0001145948
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGATGACCGAGCTGCACGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK4 (440275)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75875
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
39976642 |
+ |
EIF2AK4 |
NNNATGACCGAGCTGCACGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
4545042 |
- |
MYBBP1A |
NNNATGACCGTGCTGCAGGC |
NGG |
2 |
0.041 |
Tier I |
| 3 |
NC_000010.11 |
10 |
71802988 |
- |
CDH23 |
NNNATGACCGAGGTGCCCGC |
NGG |
2 |
0.0241 |
Tier I |
| 4 |
NC_000009.12 |
9 |
132510726 |
+ |
CFAP77 |
NNNAGGACAGAGCTGCACGC |
NGG |
2 |
0.5486 |
Tier II |
| 5 |
NC_000005.10 |
5 |
173789661 |
+ |
LINC01485 |
NNNATGTCCGAGCTGCACAC |
NGG |
2 |
0.3125 |
Tier III |
| 6 |
NC_000005.10 |
5 |
173789661 |
+ |
LOC107986482 |
NNNATGTCCGAGCTGCACAC |
NGG |
2 |
0.3125 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
43452158 |
- |
Cd72 |
NNNATGACCAAGCTGCACAC |
NGG |
2 |
0.6667 |
Tier II |
| 2 |
NC_000071.6 |
5 |
117694800 |
+ |
Ksr2 |
NNNAGGACCCAGCTGCACGC |
NGG |
2 |
0.256 |
Tier II |
| 3 |
NC_000083.6 |
17 |
90766489 |
+ |
Nrxn1 |
NNNATGACGGAGCTCCACGC |
NGG |
2 |
0.1688 |
Tier II |
| 4 |
NC_000069.6 |
3 |
132052720 |
- |
Gm29949 |
NNNAGGAACGAGCTGCACGC |
NGG |
2 |
0.416 |
Tier III |
Other clones with same target sequence:
(none)
