Construct: sgRNA BRDN0001145952
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCCCTTAACCAGCAGACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3C2B (5287)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76714
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
204442554 |
+ |
PIK3C2B |
NNNCCTTAACCAGCAGACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
134504274 |
- |
ZFAT |
NNNCCCTAAACAGCAGACTG |
NGG |
2 |
0.7879 |
Tier II |
| 3 |
NC_000002.12 |
2 |
138763797 |
+ |
NXPH2 |
NNNACTGAACCAGCAGACTG |
NGG |
2 |
0.4954 |
Tier II |
| 4 |
NC_000001.11 |
1 |
147646105 |
+ |
ACP6 |
NNNCAGTAACCAGCAGACTG |
NGG |
2 |
0.3265 |
Tier II |
| 5 |
NC_000008.11 |
8 |
27887388 |
+ |
SCARA5 |
NNNCCCTAACCTGCAGACTG |
NGG |
2 |
0.303 |
Tier II |
| 6 |
NC_000010.11 |
10 |
7351346 |
+ |
SFMBT2 |
NNNCCTTAACCAGAAGAATG |
NGG |
2 |
0.1885 |
Tier II |
| 7 |
NC_000019.10 |
19 |
18613379 |
- |
TMEM59L |
NNNCCTTACCCAGCAGAGTG |
NGG |
2 |
0.0762 |
Tier II |
| 8 |
NC_000020.11 |
20 |
10276883 |
+ |
SNAP25 |
NNNCCTTTACCAGCAGACTG |
NTG |
2 |
0.0167 |
Tier II |
| 9 |
NC_000004.12 |
4 |
127934173 |
+ |
MFSD8 |
NNNCTTTAACCAGGAGACTG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000001.11 |
1 |
221360907 |
+ |
LOC105372932 |
NNNCCTAAACCAGTAGACTG |
NGG |
2 |
0.6417 |
Tier III |
| 11 |
NC_000010.11 |
10 |
7351346 |
+ |
LOC124902372 |
NNNCCTTAACCAGAAGAATG |
NGG |
2 |
0.1885 |
Tier III |
| 12 |
NC_000022.11 |
22 |
46295797 |
- |
GTSE1-DT |
NNNCCTTCACCAGAAGACTG |
NGG |
2 |
0.15 |
Tier III |
| 13 |
NC_000001.11 |
1 |
103680427 |
- |
LOC124904594 |
NNNCCTTAACCATCAGTCTG |
NGG |
2 |
0.04 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
46068166 |
- |
Snx25 |
NNNCATTAACCAGCTGACTG |
NGG |
2 |
0.1143 |
Tier I |
| 2 |
NC_000075.6 |
9 |
54616073 |
- |
Acsbg1 |
NNNCCTTCACCAGCAGACTG |
NCG |
2 |
0.0459 |
Tier I |
| 3 |
NC_000082.6 |
16 |
90768210 |
+ |
Urb1 |
NNNCCTTACCCAGCACACTG |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000083.6 |
17 |
8546410 |
- |
Pde10a |
NNNTCTAAACCAGCAGACTG |
NGG |
2 |
0.7 |
Tier II |
| 5 |
NC_000074.6 |
8 |
56017503 |
- |
Glra3 |
NNNCCTTAACAAGCAGACTT |
NGG |
2 |
0.525 |
Tier II |
| 6 |
NC_000067.6 |
1 |
194712833 |
- |
Plxna2 |
NNNGCTTAACCAGAAGACTG |
NGG |
2 |
0.175 |
Tier II |
| 7 |
NC_000074.6 |
8 |
119743555 |
+ |
Atp2c2 |
NNNCCTTAACCAGCAGTTTG |
NGG |
2 |
0.0857 |
Tier II |
| 8 |
NC_000077.6 |
11 |
76278914 |
+ |
Nxn |
NNNCCTTAACCAGCTGACTC |
NGG |
2 |
0.0857 |
Tier II |
| 9 |
NC_000071.6 |
5 |
31512638 |
+ |
Gpn1 |
NNNCCTTTACCAGCAGCCTG |
NGG |
2 |
0.0756 |
Tier II |
| 10 |
NC_000072.6 |
6 |
46991611 |
+ |
Cntnap2 |
NNNCCTTAACCAGCAGACAG |
NAG |
2 |
0.0741 |
Tier II |
| 11 |
NC_000071.6 |
5 |
67831552 |
+ |
Atp8a1 |
NNNCCTTAACCAGGAGACTG |
NGT |
2 |
0.0 |
Tier II |
| 12 |
NC_000075.6 |
9 |
27355088 |
+ |
Igsf9b |
NNNCCTTAACCAGGAGACTT |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000068.7 |
2 |
134971287 |
+ |
Plcb1 |
NNNCCTTAACCAGGGGACTG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000078.6 |
12 |
72656194 |
- |
Dhrs7 |
NNNCCTTAAGCAGGAGACTG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000081.6 |
15 |
103127488 |
+ |
D930007P13Rik |
NNNCCATAACAAGCAGACTG |
NGG |
2 |
0.65 |
Tier III |
| 16 |
NC_000067.6 |
1 |
194712833 |
- |
Gm46202 |
NNNGCTTAACCAGAAGACTG |
NGG |
2 |
0.175 |
Tier III |
| 17 |
NC_000083.6 |
17 |
26856447 |
- |
LOC115488773 |
NNNCCTTAACCAGCAGGCTA |
NGG |
2 |
0.1654 |
Tier III |
| 18 |
NC_000071.6 |
5 |
67831552 |
+ |
Gm52816 |
NNNCCTTAACCAGGAGACTG |
NGT |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
