Construct: sgRNA BRDN0001145955

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTGGAAAAAGGCTTCTACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK1 (8536)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77476

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 9763006 - CAMK1 NNNGGAAAAAGGCTTCTACA NGG 0 1.0 Tier I
2 NC_000013.11 13 60396255 - TDRD3 NNNGGAAAAAGGCTTCTAAG NGG 2 0.3277 Tier I
3 NC_000007.14 7 45704537 + ADCY1 NNNGGAAAAAGACTTTTACA NGG 2 0.2872 Tier I
4 NC_000003.12 3 9763006 - OGG1 NNNGGAAAAAGGCTTCTACA NGG 0 1.0 Tier II
5 NC_000013.11 13 28946905 - MTUS2 NNNGGAAAATGACTTCTACA NGG 2 0.8235 Tier II
6 NC_000012.12 12 77541 + IQSEC3 NNNGAAAAAAGACTTCTACA NGG 2 0.8089 Tier II
7 NC_000002.12 2 201529064 + C2CD6 NNNGGTAAAAGACTTCTACA NGG 2 0.6667 Tier II
8 NC_000009.12 9 19671250 - SLC24A2 NNNGGTAAAAGACTTCTACA NGG 2 0.6667 Tier II
9 NC_000009.12 9 20173660 + SLC24A2 NNNGGTAAAAGACTTCTACA NGG 2 0.6667 Tier II
10 NC_000017.11 17 37105197 - ACACA NNNGGAAAAAGATTTCTACA NGG 2 0.6533 Tier II
11 NC_000006.12 6 145887234 - SHPRH NNNGGAAAGAGGCTTCCACA NGG 2 0.4706 Tier II
12 NC_000011.10 11 24743392 - LUZP2 NNNGGCAAAAGGCTTCTACA NGG 1 0.4545 Tier II
13 NC_000002.12 2 63910437 - VPS54 NNNGGACAAAGGCTTCTACA NGG 1 0.4375 Tier II
14 NC_000003.12 3 125304180 + ZNF148 NNNGGAAGAGGGCTTCTACA NGG 2 0.4074 Tier II
15 NC_000001.11 1 43850492 - ST3GAL3 NNNGGAAAAAGGCTTCCAAA NGG 2 0.3025 Tier II
16 NC_000002.12 2 49058763 + FSHR NNNGGAAAAAGGCCTCTACA NGG 1 0.2857 Tier II
17 NC_000001.11 1 24461033 + NIPAL3 NNNGGAAAAATGCTTCAACA NGG 2 0.2051 Tier II
18 NC_000007.14 7 156878519 + LMBR1 NNNGGTAAAAGGCCTCTACA NGG 2 0.2041 Tier II
19 NC_000001.11 1 70866024 - PTGER3 NNNGGAAACAGGCTTTTACA NGG 2 0.1758 Tier II
20 NC_000020.11 20 8689046 - PLCB1 NNNGTCAAAAGGCTTCTACA NGG 2 0.1364 Tier II
21 NC_000020.11 20 20123934 - CFAP61 NNNGGCAAAAGGCCTCTACA NGG 2 0.1299 Tier II
22 NC_000017.11 17 48256029 + SKAP1 NNNGGAAAAAGGCTTCTGCA NAG 2 0.1037 Tier II
23 NC_000007.14 7 73668784 + VPS37D NNNGGAAAAAGCCTTCTCCA NGG 2 0.1008 Tier II
24 NC_000009.12 9 28387505 - LINGO2 NNNAGAAAAAGGCTTCTACA NCG 2 0.0964 Tier II
25 NC_000018.10 18 10744389 - PIEZO2 NNNGGGAAAAGGCTTCTAGA NGG 2 0.0893 Tier II
26 NC_000019.10 19 5078171 - KDM4B NNNGGACAAAGGCTTCTCCA NGG 2 0.0833 Tier II
27 NC_000019.10 19 48896800 - TULP2 NNNGGAACAAGGCTTCTAGA NGG 2 0.0536 Tier II
28 NC_000008.11 8 112413411 - CSMD3 NNNGGAAAAAGGATTCGACA NGG 2 0.0452 Tier II
29 NC_000024.10 Y 6918075 + TBL1Y NNNGGAAAAAGGCTTCTAAA NTG 2 0.0167 Tier II
30 NC_000004.12 4 184776311 - ACSL1 NNNGGAAAAAGGATTCTACA NGT 2 0.0062 Tier II
31 NC_000005.10 5 169965949 + DOCK2 NNNGGAAAAAGGCTTCTCCA NGC 2 0.0042 Tier II
32 NC_000005.10 5 169965949 + INSYN2B NNNGGAAAAAGGCTTCTCCA NGC 2 0.0042 Tier II
33 NC_000007.14 7 111047556 - IMMP2L NNNGGAAAAAGGCTTCTACA NTT 2 0.0 Tier II
34 NC_000005.10 5 17902125 - LINC02223 NNNGGTAAAAGACTTCTACA NGG 2 0.6667 Tier III
35 NC_000001.11 1 112278030 + LINC02884 NNNGGAAGAAGGCTTCTACT NGG 2 0.44 Tier III
36 NC_000020.11 20 21401291 - LOC105372558 NNNGGAAAAAGGCTTATAAA NGG 2 0.4286 Tier III
37 NC_000002.12 2 34265449 + LINC01317 NNNGGCAAAAGACTTCTACA NGG 2 0.4242 Tier III
38 NC_000006.12 6 18666405 - MIR548A1HG NNNGCAAAAAGGCTTCTGCA NGG 2 0.3143 Tier III
39 NC_000010.11 10 45447117 + LOC102724323 NNNGGGTAAAGGCTTCTACA NGG 2 0.3125 Tier III
40 NC_000001.11 1 43850492 - SHMT1P1 NNNGGAAAAAGGCTTCCAAA NGG 2 0.3025 Tier III
41 NC_000003.12 3 84711874 + LINC00971 NNNGGAAAAAGACCTCTACA NGG 2 0.2667 Tier III
42 NC_000001.11 1 60660500 + LOC101926964 NNNGGAAACAGGCTTCTGCA NGG 2 0.2286 Tier III
43 NC_000005.10 5 103510200 - PDZPH1P NNNGGAAAAAGGATTCTACA NAG 2 0.0997 Tier III
44 NC_000002.12 2 200110471 - LOC124906112 NNNGGAACAAGGCTTCTAGA NGG 2 0.0536 Tier III
45 NC_000006.12 6 133088359 + LINC00326 NNNGGAAAAAGGCTTCTCCA NAG 2 0.0494 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 58954437 - Pdzph1 NNNAGAAAAAGGATTCTACA NGG 2 0.3462 Tier I
2 NC_000072.6 6 113339377 - Camk1 NNNGGAGAAAGGATTCTACA NGG 2 0.2715 Tier I
3 NC_000077.6 11 70321753 + Alox12e NNNGGAAAAAGGCTTCTCCT NGG 2 0.1143 Tier I
4 NC_000080.6 14 69980431 - Pebp4 NNNGAAAGAAGGCTTCTACA NGG 2 0.6356 Tier II
5 NC_000073.6 7 56748257 + Gabrg3 NNNGGAAAAAGACTTCTAAA NGG 2 0.4 Tier II
6 NC_000073.6 7 112887308 + Tead1 NNNGGAAAGGGGCTTCTACA NGG 2 0.3704 Tier II
7 NC_000076.6 10 76293009 + Dip2a NNNGGAAACAGGCATCTACA NGG 2 0.3537 Tier II
8 NC_000073.6 7 68101772 - Igf1r NNNGGAAAAAGGCTTCCTCA NGG 2 0.3529 Tier II
9 NC_000082.6 16 92629111 + Runx1 NNNGAAAAAATGCTTCTACA NGG 2 0.3333 Tier II
10 NC_000071.6 5 136318811 + Cux1 NNNGGAATAAGGCATCTACA NGG 2 0.2653 Tier II
11 NC_000076.6 10 76802835 + Pcbp3 NNNTGAAAAAGGCTTCAACA NGG 2 0.1939 Tier II
12 NC_000081.6 15 58597694 + Fer1l6 NNNGGGAAAAGGCTTCTACA NAG 2 0.1852 Tier II
13 NC_000073.6 7 110966048 + Mrvi1 NNNGGAAAAAGACTTCTCCA NGG 2 0.1778 Tier II
14 NC_000069.6 3 136272167 + Bank1 NNNGGAAAACCGCTTCTACA NGG 2 0.1429 Tier II
15 NC_000073.6 7 78482584 - Ntrk3 NNNGTAAAAAGGCTTCTACC NGG 2 0.0682 Tier II
16 NC_000081.6 15 97275052 + Pced1b NNNGGAAAAAGGCTCCTCCA NGG 2 0.0519 Tier II
17 NC_000078.6 12 56857544 + Slc25a21 NNNGGAAGAAGGCTTCTACA NGA 2 0.0509 Tier II
18 NC_000072.6 6 14989153 + Foxp2 NNNGGAAATAGGCTTCTACA NGA 2 0.0417 Tier II
19 NC_000076.6 10 10828294 + Grm1 NNNGGAAAGAGGCTTCTACA NGC 2 0.0148 Tier II
20 NC_000080.6 14 69980431 - Gm41198 NNNGAAAGAAGGCTTCTACA NGG 2 0.6356 Tier III
21 NC_000079.6 13 74527139 - Gm20590 NNNAGAAAAATGCTTCTACA NGG 2 0.3462 Tier III
22 NC_000079.6 13 76019939 - Gm4165 NNNGCAAAAAGGCTTCTACA NAG 2 0.2037 Tier III
23 NC_000068.7 2 27145911 + Dbhos NNNGGAAAGAGGCTTCTACA NAG 2 0.1728 Tier III
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Other clones with same target sequence:

(none)