Construct: sgRNA BRDN0001145957

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATCGCATCATTGGGCGCGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRK2 (156)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75682

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 67281134 + GRK2 NNNCGCATCATTGGGCGCGG NGG 0 1.0 Tier I
2 NC_000010.11 10 3129719 + PFKP NNNCGCATGTTTGGGCGCGG NGG 2 0.5462 Tier II
3 NC_000022.11 22 22228498 - IGL NNNAGCATCACTGGGCGCGG NGG 2 0.5614 Tier III
4 NC_000009.12 9 97238204 + ANKRD18CP NNNCGCAGCAGTGGGCGCGG NGG 2 0.2933 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 39562895 + Gm33989 NNNCGCATCATTGGGGGTGG NGG 2 0.0989 Tier II
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Other clones with same target sequence:

(none)