Construct: sgRNA BRDN0001145959
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATGAGCATCATTCGCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GNE (10020)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77832
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
36246041 |
- |
GNE |
NNNTGAGCATCATTCGCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
23863328 |
+ |
UBE2E1 |
NNNTGAGCATTATTCACATG |
NGG |
2 |
0.3077 |
Tier II |
| 3 |
NC_000003.12 |
3 |
140463949 |
+ |
CLSTN2 |
NNNAGAGCATCTTTCGCATG |
NGG |
2 |
0.1587 |
Tier II |
| 4 |
NC_000006.12 |
6 |
124791179 |
+ |
NKAIN2 |
NNNTGAGCATCATTTGAATG |
NGG |
2 |
0.0311 |
Tier II |
| 5 |
NC_000002.12 |
2 |
45749422 |
+ |
PRKCE |
NNNAGAGCATCATTGGCATG |
NGG |
2 |
0.0238 |
Tier II |
| 6 |
NC_000001.11 |
1 |
34172942 |
+ |
C1orf94 |
NNNTGAGCATTATTCGCATG |
NGA |
2 |
0.0214 |
Tier II |
| 7 |
NC_000016.10 |
16 |
7579522 |
- |
RBFOX1 |
NNNGGAGCATCATTCTCATG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000015.10 |
15 |
38345645 |
- |
SPRED1 |
NNNTGAGCATCATTCCCATG |
NGA |
2 |
0.0 |
Tier II |
| 9 |
NC_000003.12 |
3 |
140463949 |
+ |
LOC105374132 |
NNNAGAGCATCTTTCGCATG |
NGG |
2 |
0.1587 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
44059786 |
- |
Gne |
NNNTGAGCATCATTCGGATG |
NGG |
1 |
0.0588 |
Tier I |
| 2 |
NC_000077.6 |
11 |
46274450 |
+ |
Cyfip2 |
NNNTGAGCATCACTCACATG |
NGG |
2 |
0.7895 |
Tier II |
| 3 |
NC_000078.6 |
12 |
89151983 |
- |
Nrxn3 |
NNNTGAGCATCATTAGCATA |
NGG |
2 |
0.2083 |
Tier II |
| 4 |
NC_000075.6 |
9 |
96832582 |
- |
Pxylp1 |
NNNTGAGCATCTTTAGCATG |
NGG |
2 |
0.0741 |
Tier II |
| 5 |
NC_000080.6 |
14 |
60713351 |
+ |
Spata13 |
NNNTGAGCATCATGCCCATG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000070.6 |
4 |
110926703 |
+ |
Agbl4 |
NNNTGAGCATCTTTCCCATG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000075.6 |
9 |
92612599 |
+ |
Gm2495 |
NNNTGAGCATCATGGGCATG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
