Construct: sgRNA BRDN0001145964
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGGCCTTACCGAGCTCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SPEG (10290)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77625
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
219449266 |
- |
SPEG |
NNNGGCCTTACCGAGCTCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
75213145 |
+ |
ANKRD31 |
NNNGGCTTTACAGAGCTCAG |
NGG |
2 |
0.5804 |
Tier II |
| 3 |
NC_000006.12 |
6 |
44258571 |
+ |
NFKBIE |
NNNGGCCCTACCGTGCTCAG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000006.12 |
6 |
44258571 |
+ |
POLR1C |
NNNGGCCCTACCGTGCTCAG |
NGG |
2 |
0.5333 |
Tier II |
| 5 |
NC_000011.10 |
11 |
2441345 |
- |
TRPM5 |
NNNGGCCTGACCGAGCCCAG |
NGG |
2 |
0.437 |
Tier II |
| 6 |
NC_000001.11 |
1 |
36872924 |
- |
GRIK3 |
NNNGGCCTTACCCAGCCCAG |
NGG |
2 |
0.2972 |
Tier II |
| 7 |
NC_000023.11 |
X |
107544698 |
+ |
FRMPD3 |
NNNGGCCTTACAGAGCTCAG |
NAG |
2 |
0.1852 |
Tier II |
| 8 |
NC_000001.11 |
1 |
204261092 |
+ |
PLEKHA6 |
NNNGGCCTTCCCGAGCTCAG |
NGA |
2 |
0.0231 |
Tier II |
| 9 |
NC_000003.12 |
3 |
59205675 |
+ |
CFAP20DC-DT |
NNNGGTCTTACCAAGCTCAG |
NGG |
2 |
0.8571 |
Tier III |
| 10 |
NC_000024.10 |
Y |
20728454 |
- |
TBL1YP1 |
NNNGGCCTTACCGGGCACAG |
NGG |
2 |
0.2489 |
Tier III |
| 11 |
NC_000023.11 |
X |
107544698 |
+ |
FRMPD3-AS1 |
NNNGGCCTTACAGAGCTCAG |
NAG |
2 |
0.1852 |
Tier III |
| 12 |
NC_000015.10 |
15 |
93271277 |
- |
LOC105370982 |
NNNGGCCTTCCCCAGCTCAG |
NGG |
2 |
0.1404 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
30911687 |
+ |
Adamts15 |
NNNGGCCTTACCCAGCTCAT |
NGG |
2 |
0.2947 |
Tier I |
| 2 |
NC_000075.6 |
9 |
21165867 |
- |
Pde4a |
NNNGGCCTGTCCGAGCTCAG |
NGG |
2 |
0.5462 |
Tier II |
| 3 |
NC_000075.6 |
9 |
44719328 |
- |
Phldb1 |
NNNGGCCTTAACGAGCCCAG |
NGG |
2 |
0.5294 |
Tier II |
| 4 |
NC_000074.6 |
8 |
122404957 |
+ |
Zc3h18 |
NNNGGACTTACTGAGCTCAG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000068.7 |
2 |
168898817 |
- |
Zfp64 |
NNNGGCCTCACTGAGCTCAG |
NGG |
2 |
0.497 |
Tier II |
| 6 |
NC_000077.6 |
11 |
108746144 |
- |
Cep112 |
NNNGGCCTTTCCCAGCTCAG |
NGG |
2 |
0.3715 |
Tier II |
| 7 |
NC_000079.6 |
13 |
30669033 |
+ |
Dusp22 |
NNNGGCCTTCCAGAGCTCAG |
NGG |
2 |
0.2381 |
Tier II |
| 8 |
NC_000085.6 |
19 |
6095994 |
+ |
Gm550 |
NNNGGCCTTACAGAGCTCAG |
NGA |
2 |
0.0496 |
Tier II |
Other clones with same target sequence:
(none)
