Construct: sgRNA BRDN0001145971

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTATGAGAAACCTGAACGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCK (1633)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78059

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 71022430 + DCK NNNATGAGAAACCTGAACGA NGG 0 1.0 Tier I
2 NC_000006.12 6 47965063 - PTCHD4 NNNATGAGAAACCTGAATAA NGG 2 0.4592 Tier II
3 NC_000010.11 10 45765269 - WASHC2C NNNATGAGAAACCAGAACAA NGG 2 0.4422 Tier II
4 NC_000010.11 10 50105924 - WASHC2A NNNATGAGAAACCAGAACAA NGG 2 0.4422 Tier II
5 NC_000006.12 6 110438022 - SLC22A16 NNNATCAGAAACCTGAATGA NGG 2 0.4383 Tier II
6 NC_000005.10 5 79058440 + DMGDH NNNATGAGAAGCCTGAAAGA NGG 2 0.35 Tier II
7 NC_000011.10 11 115395284 - CADM1 NNNATGAGAAATCTGAAAGA NGG 2 0.2899 Tier II
8 NC_000004.12 4 181906543 + TENM3 NNNATGAGAAACCAGAAGGA NGG 2 0.0825 Tier II
9 NC_000001.11 1 213827798 + RPS6KC1 NNNATGAGACACCTGAAGGA NGG 2 0.0444 Tier II
10 NC_000015.10 15 32951771 + FMN1 NNNATGAGAAAACTGTACGA NGG 2 0.0 Tier II
11 NC_000010.11 10 45706973 + FAM21FP NNNATGAGAAACCAGAACAA NGG 2 0.4422 Tier III
12 NC_000010.11 10 50048744 + FAM21EP NNNATGAGAAACCAGAACAA NGG 2 0.4422 Tier III
13 NC_000006.12 6 135234447 + LOC105378011 NNNATGAGAAACCTGAAACA NGG 2 0.2414 Tier III
14 NC_000006.12 6 82069173 + LINC02542 NNNATGAGAAAGATGAACGA NGG 2 0.1709 Tier III
15 NC_000006.12 6 82069173 + LOC107986617 NNNATGAGAAAGATGAACGA NGG 2 0.1709 Tier III
16 NC_000001.11 1 90842716 - LINC02609 NNNATAAGAAACCTGATCGA NGG 2 0.1333 Tier III
17 NC_000001.11 1 90842716 - LINC02788 NNNATAAGAAACCTGATCGA NGG 2 0.1333 Tier III
18 NC_000006.12 6 164086686 - LOC105378106 NNNATGAGAAACCTGAACGG NGA 2 0.0531 Tier III
19 NC_000001.11 1 213827798 + PROX1-AS1 NNNATGAGACACCTGAAGGA NGG 2 0.0444 Tier III
20 NC_000013.11 13 60342511 - LOC105370228 NNNATGAGAAACGTGAAGGA NGG 2 0.0182 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 88772693 + Dck NNNATGAGAAACCTGAACGG NGG 1 0.7647 Tier I
2 NC_000070.6 4 148947104 + Casz1 NNNATGAGAAGGCTGAACGA NGG 2 0.2889 Tier I
3 NC_000079.6 13 41157451 + Sycp2l NNNTTGAGAAACCTGAACGA NGA 2 0.0442 Tier I
4 NC_000082.6 16 14138451 - Marf1 NNNATGAGAAACCTGCACGA NGC 2 0.0 Tier I
5 NC_000072.6 6 140593804 - Plekha5 NNNATGAGAAACCTGAACTA NGG 1 0.6667 Tier II
6 NC_000068.7 2 10170608 - Itih5 NNNATGAGAAATCTGAACGA NGG 1 0.5385 Tier II
7 NC_000077.6 11 110431493 + Map2k6 NNNATGAGAAACCTGAACTT NGG 2 0.4 Tier II
8 NC_000073.6 7 43723528 - Klk13 NNNATGAGAAACCTGAAAGT NGG 2 0.3231 Tier II
9 NC_000074.6 8 122979077 + Ankrd11 NNNATGAGAAAACTGAACCA NGG 2 0.3202 Tier II
10 NC_000084.6 18 35598962 - Paip2 NNNATGAGAAAAATGAACGA NGG 2 0.2747 Tier II
11 NC_000070.6 4 149002747 - Pex14 NNNATGAGAAACCTGAACAA NAG 2 0.1852 Tier II
12 NC_000073.6 7 134125179 - Adam12 NNNATGAGAATCCTGAAAGA NGG 2 0.1657 Tier II
13 NC_000076.6 10 75762066 + Cabin1 NNNATGAGGAACCTGAAGGA NGG 2 0.0889 Tier II
14 NC_000079.6 13 100606199 - Marveld2 NNNATGAGAAACCTGATGGA NGG 2 0.0178 Tier II
15 NC_000073.6 7 105579100 - Apbb1 NNNATGAGAAACATGAACGA NTG 2 0.015 Tier II
16 NC_000078.6 12 52565795 + Arhgap5 NNNATGAGAAACCGGATCGA NGG 2 0.0 Tier II
17 NC_000073.6 7 143560941 + Cars NNNATGAGAAACCTGCACCA NGG 2 0.0 Tier II
18 NC_000076.6 10 24672232 - Enpp1 NNNATGAGAAACCTTCACGA NGG 2 0.0 Tier II
19 NC_000078.6 12 39494426 - Gm34923 NNNATGAGAAACATGAATGA NGG 2 0.2473 Tier III
20 NC_000079.6 13 90486077 - Gm35111 NNNATGAGACACCTGAATGA NGG 2 0.2143 Tier III
21 NC_000070.6 4 149002747 - Gm42351 NNNATGAGAAACCTGAACAA NAG 2 0.1852 Tier III
Download CSV

Other clones with same target sequence:

(none)