Construct: sgRNA BRDN0001145974
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGACAGCATACTCCATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA1 (6195)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75497
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
26560835 |
- |
RPS6KA1 |
NNNGACAGCATACTCCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
48423546 |
+ |
PLXNB1 |
NNNGACATCATACTCGATGT |
NGG |
2 |
0.0962 |
Tier I |
| 3 |
NC_000006.12 |
6 |
75145401 |
+ |
COL12A1 |
NNNGACTGCATACTCCGTGT |
NGG |
2 |
0.0772 |
Tier I |
| 4 |
NC_000018.10 |
18 |
37051085 |
- |
KIAA1328 |
NNNGACAGAATACTCAATGT |
NGG |
2 |
0.8571 |
Tier II |
| 5 |
NC_000007.14 |
7 |
45691308 |
- |
ADCY1 |
NNNGGCAGTATACTCCATGT |
NGG |
2 |
0.63 |
Tier II |
| 6 |
NC_000016.10 |
16 |
23840026 |
+ |
PRKCB |
NNNGACAGCATATTCCATTT |
NGG |
2 |
0.4667 |
Tier II |
| 7 |
NC_000014.9 |
14 |
102989690 |
- |
CDC42BPB |
NNNGACAGGGTACTCCATGT |
NGG |
2 |
0.3439 |
Tier II |
| 8 |
NC_000020.11 |
20 |
44537304 |
+ |
PKIG |
NNNGACAGCATTCTCCATGT |
NGG |
1 |
0.3333 |
Tier II |
| 9 |
NC_000010.11 |
10 |
12003200 |
- |
UPF2 |
NNNGACTGCATGCTCCATGT |
NGG |
2 |
0.316 |
Tier II |
| 10 |
NC_000004.12 |
4 |
164184849 |
- |
MARCHF1 |
NNNGACAACATACTCTATGT |
NGG |
2 |
0.3077 |
Tier II |
| 11 |
NC_000006.12 |
6 |
16621539 |
- |
ATXN1 |
NNNGACGGCATTCTCCATGT |
NGG |
2 |
0.2353 |
Tier II |
| 12 |
NC_000016.10 |
16 |
74531528 |
+ |
GLG1 |
NNNGACAGGATTCTCCATGT |
NGG |
2 |
0.2063 |
Tier II |
| 13 |
NC_000019.10 |
19 |
40472110 |
+ |
SPTBN4 |
NNNGACAGCGTTCTCCATGT |
NGG |
2 |
0.1852 |
Tier II |
| 14 |
NC_000017.11 |
17 |
72649127 |
- |
SLC39A11 |
NNNCACAGCATCCTCCATGT |
NGG |
2 |
0.1393 |
Tier II |
| 15 |
NC_000007.14 |
7 |
31746789 |
- |
PDE1C |
NNNGACAGCATAGTCCATGT |
NGG |
1 |
0.1364 |
Tier II |
| 16 |
NC_000008.11 |
8 |
141361081 |
+ |
GPR20 |
NNNGACAGCAGACTCCATGG |
NGG |
2 |
0.0706 |
Tier II |
| 17 |
NC_000011.10 |
11 |
79240461 |
- |
TENM4 |
NNNGACAGCATAGTCCAGGT |
NGG |
2 |
0.0455 |
Tier II |
| 18 |
NC_000005.10 |
5 |
7471185 |
+ |
ADCY2 |
NNNGACAGCATACATCATGT |
NGG |
2 |
0.0413 |
Tier II |
| 19 |
NC_000016.10 |
16 |
72865561 |
- |
ZFHX3 |
NNNGACAGCATACTCCTTGT |
NAG |
2 |
0.0346 |
Tier II |
| 20 |
NC_000015.10 |
15 |
25090523 |
+ |
SNHG14 |
NNNGACAGCATATTCCATGT |
NGG |
1 |
0.7 |
Tier III |
| 21 |
NC_000001.11 |
1 |
165941332 |
+ |
LOC100420658 |
NNNGACAGCATACTCCAGGC |
NGG |
2 |
0.0303 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
133860834 |
+ |
Rps6ka1 |
NNNGACAGCATACTCCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000078.6 |
12 |
4322918 |
- |
Ncoa1 |
NNNTACAGCATACTGCATGT |
NGG |
2 |
0.0182 |
Tier I |
| 3 |
NC_000071.6 |
5 |
96737093 |
- |
Fras1 |
NNNGCCAGCATACTCCATGT |
NGT |
2 |
0.0081 |
Tier I |
| 4 |
NC_000071.6 |
5 |
113189523 |
- |
2900026A02Rik |
NNNGACAGCATGCTCCATGC |
NGG |
2 |
0.0657 |
Tier II |
| 5 |
NC_000070.6 |
4 |
104259401 |
- |
Dab1 |
NNNGACAGCATCCTCCATGG |
NGG |
2 |
0.0464 |
Tier II |
| 6 |
NC_000082.6 |
16 |
7400843 |
- |
Rbfox1 |
NNNGACAGCACACTCCATGT |
NGA |
2 |
0.0463 |
Tier II |
| 7 |
NC_000074.6 |
8 |
123513853 |
+ |
Dbndd1 |
NNNGACAGCATACTCCATCT |
NTG |
2 |
0.0175 |
Tier II |
| 8 |
NC_000077.6 |
11 |
6335788 |
- |
Ogdh |
NNNGACAGCATACTCAATGT |
NGT |
2 |
0.0161 |
Tier II |
| 9 |
NC_000081.6 |
15 |
80381464 |
+ |
Cacna1i |
NNNGACAGCATAATCCATGT |
NGT |
2 |
0.0062 |
Tier II |
| 10 |
NC_000068.7 |
2 |
113354661 |
- |
Fmn1 |
NNNGACAGCCTACTCCATGT |
NGT |
2 |
0.0054 |
Tier II |
| 11 |
NC_000083.6 |
17 |
72182350 |
- |
Alk |
NNNGACAGCATACTCCATGT |
NCA |
2 |
0.0 |
Tier II |
| 12 |
NC_000067.6 |
1 |
131254054 |
+ |
Gm28913 |
NNNGAGAGCATCCTCCATGT |
NGG |
2 |
0.1316 |
Tier III |
| 13 |
NC_000071.6 |
5 |
96737093 |
- |
Gm32995 |
NNNGCCAGCATACTCCATGT |
NGT |
2 |
0.0081 |
Tier III |
Other clones with same target sequence:
(none)
