Construct: sgRNA BRDN0001145981
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCAGCTTTCCGAATCATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K7 (6885)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75692
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
90553476 |
- |
MAP3K7 |
NNNAGCTTTCCGAATCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
96337452 |
- |
NR2F2 |
NNNAGCTTTCCGAATCTCGT |
NGG |
2 |
0.0571 |
Tier I |
| 3 |
NC_000020.11 |
20 |
25358107 |
+ |
ABHD12 |
NNNAACTTTCCTAATCATGT |
NGG |
2 |
0.3333 |
Tier II |
| 4 |
NC_000006.12 |
6 |
157306326 |
+ |
TMEM242 |
NNNAGCTGTCCGAATCATCT |
NGG |
2 |
0.3287 |
Tier II |
| 5 |
NC_000009.12 |
9 |
32553187 |
- |
NDUFB6 |
NNNAGCTTTCCGAATCATTT |
NTG |
2 |
0.026 |
Tier II |
| 6 |
NC_000009.12 |
9 |
32553187 |
- |
SMIM27 |
NNNAGCTTTCCGAATCATTT |
NTG |
2 |
0.026 |
Tier II |
| 7 |
NC_000005.10 |
5 |
150963990 |
+ |
LOC124901114 |
NNNAGCTTTCCTAATAATGT |
NGG |
2 |
0.3846 |
Tier III |
| 8 |
NC_000023.11 |
X |
94570089 |
- |
LOC107985704 |
NNNAGCTTTCCTAATAATGT |
NGG |
2 |
0.3846 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
31985797 |
+ |
Map3k7 |
NNNAGCTTTCAGAATCATGT |
NGG |
1 |
0.75 |
Tier I |
| 2 |
NC_000073.6 |
7 |
70354828 |
+ |
Nr2f2 |
NNNAGCTTTCCGAACCGTGT |
NGG |
2 |
0.0481 |
Tier I |
| 3 |
NC_000085.6 |
19 |
46731019 |
+ |
As3mt |
NNNGGCTTTCCGATTCATGT |
NGG |
2 |
0.3333 |
Tier II |
| 4 |
NC_000084.6 |
18 |
21968350 |
+ |
Ccdc178 |
NNNAACTTTCTGAATCATGT |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000080.6 |
14 |
86841085 |
+ |
Diaph3 |
NNNTGCTTTCCGCATCATGT |
NGG |
2 |
0.134 |
Tier II |
| 6 |
NC_000080.6 |
14 |
17916237 |
- |
Thrb |
NNNAGCTTTCCGAATCATCT |
NTG |
2 |
0.0175 |
Tier II |
| 7 |
NC_000073.6 |
7 |
82538483 |
- |
Adamtsl3 |
NNNAGCTTTCCGAATCCTGT |
NTG |
2 |
0.0069 |
Tier II |
| 8 |
NC_000070.6 |
4 |
125472295 |
+ |
Gm32756 |
NNNAGCTTTTCGAATCATCT |
NGG |
2 |
0.4219 |
Tier III |
| 9 |
NC_000083.6 |
17 |
9377445 |
- |
Gm7947 |
NNNAGTTTTCCTAATCATGT |
NGG |
2 |
0.3571 |
Tier III |
Other clones with same target sequence:
(none)
