Construct: sgRNA BRDN0001145982
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGACTCGGATCTCCTTCTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R5 (23533)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75758
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
8904882 |
- |
PIK3R5 |
NNNCTCGGATCTCCTTCTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
57179360 |
- |
LRP1 |
NNNCTCGGATCTCCATCTGA |
NGT |
2 |
0.0093 |
Tier I |
| 3 |
NC_000007.14 |
7 |
40549072 |
+ |
SUGCT |
NNNCTTGGATCTCCTTCTGA |
NGG |
1 |
0.9286 |
Tier II |
| 4 |
NC_000003.12 |
3 |
59006274 |
- |
CFAP20DC |
NNNCTCCCATCTCCTTCTGA |
NGG |
2 |
0.4231 |
Tier II |
| 5 |
NC_000006.12 |
6 |
7870510 |
+ |
BMP6 |
NNNCTCTGTTCTCCTTCTGA |
NGG |
2 |
0.3429 |
Tier II |
| 6 |
NC_000023.11 |
X |
40132203 |
+ |
BCOR |
NNNCTCGGATATCCTTCCGA |
NGG |
2 |
0.3214 |
Tier II |
| 7 |
NC_000007.14 |
7 |
88782272 |
- |
ZNF804B |
NNNCTCTGATCTCCTTCTGA |
NAG |
2 |
0.1481 |
Tier II |
| 8 |
NC_000004.12 |
4 |
98477512 |
+ |
TSPAN5 |
NNNCTCGGCTCTGCTTCTGA |
NGG |
2 |
0.0779 |
Tier II |
| 9 |
NC_000017.11 |
17 |
2377688 |
- |
SGSM2 |
NNNCTCGGATCTCCTTGTGG |
NGG |
2 |
0.045 |
Tier II |
| 10 |
NC_000016.10 |
16 |
83818216 |
- |
HSBP1 |
NNNCTCGCATCTCCTTCTGA |
NGA |
2 |
0.0427 |
Tier II |
| 11 |
NC_000002.12 |
2 |
154591336 |
- |
LOC105373693 |
NNNCTCTAATCTCCTTCTGA |
NGG |
2 |
0.5714 |
Tier III |
| 12 |
NC_000003.12 |
3 |
59006274 |
- |
CFAP20DC-AS1 |
NNNCTCCCATCTCCTTCTGA |
NGG |
2 |
0.4231 |
Tier III |
| 13 |
NC_000005.10 |
5 |
93576733 |
+ |
NR2F1-AS1 |
NNNCTCAGATCTACTTCTGA |
NGG |
2 |
0.3846 |
Tier III |
| 14 |
NC_000008.11 |
8 |
57507916 |
+ |
LOC105375855 |
NNNCTGGGCTCTCCTTCTGA |
NGG |
2 |
0.2857 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
134503019 |
+ |
Clip2 |
NNNCTCCGATCTCCTTCTGC |
NGG |
2 |
0.1563 |
Tier I |
| 2 |
NC_000081.6 |
15 |
84676737 |
- |
Prr5 |
NNNCTAGGATCTCCTTCTAA |
NGG |
2 |
0.6633 |
Tier II |
| 3 |
NC_000071.6 |
5 |
127416754 |
+ |
Tmem132c |
NNNCACGGATCTCCTACTGA |
NGG |
2 |
0.4545 |
Tier II |
| 4 |
NC_000084.6 |
18 |
43344780 |
+ |
Dpysl3 |
NNNCACGGATCTCCTACTGA |
NGG |
2 |
0.4545 |
Tier II |
| 5 |
NC_000074.6 |
8 |
79342038 |
- |
Smad1 |
NNNCTCAGATCTCCTTCTCA |
NGG |
2 |
0.4483 |
Tier II |
| 6 |
NC_000070.6 |
4 |
86054731 |
+ |
Adamtsl1 |
NNNCTCAGATCTACTTCTGA |
NGG |
2 |
0.3846 |
Tier II |
| 7 |
NC_000068.7 |
2 |
141010238 |
- |
Macrod2 |
NNNCTGGGATCTCCTTCTTA |
NGG |
2 |
0.3333 |
Tier II |
| 8 |
NC_000077.6 |
11 |
96565030 |
- |
Skap1 |
NNNCTCGGGTCTCCTTCTGA |
NTG |
2 |
0.026 |
Tier II |
| 9 |
NC_000071.6 |
5 |
100227139 |
- |
Gm8091 |
NNNCTGGGATCTCCTGCTGA |
NGG |
2 |
0.1731 |
Tier III |
Other clones with same target sequence:
(none)
