Construct: sgRNA BRDN0001145987
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATATGCATTCAATAATGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRPPRC (10128)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77773
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
43975140 |
+ |
LRPPRC |
NNNATGCATTCAATAATGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
182241321 |
+ |
TENM3 |
NNNATTCATTCAATAATGAG |
NGG |
2 |
0.2857 |
Tier II |
| 3 |
NC_000006.12 |
6 |
25744705 |
+ |
SLC17A1 |
NNNCTGCATTCAATAATGTG |
NGG |
2 |
0.1629 |
Tier II |
| 4 |
NC_000014.9 |
14 |
37706592 |
+ |
TTC6 |
NNNATGCATTCAATAATGAG |
NTG |
2 |
0.0167 |
Tier II |
| 5 |
NC_000010.11 |
10 |
115521114 |
- |
ATRNL1 |
NNNATGCATTCAATAATGCT |
NGT |
2 |
0.0113 |
Tier II |
| 6 |
NC_000008.11 |
8 |
139640922 |
- |
KCNK9 |
NNNATGCATTCAATAATGAG |
NGC |
2 |
0.0095 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
94361419 |
+ |
Ttc17 |
NNNATGCATTCAATAATGAG |
NGG |
1 |
0.4286 |
Tier II |
| 2 |
NC_000083.6 |
17 |
57541785 |
+ |
Vmn2r120 |
NNNATGCATTCAATAATACC |
NGG |
2 |
0.2967 |
Tier II |
| 3 |
NC_000074.6 |
8 |
125740081 |
- |
Ntpcr |
NNNATGCATTCCATAATGCG |
NGA |
2 |
0.0183 |
Tier II |
| 4 |
NC_000074.6 |
8 |
12885902 |
- |
Mcf2l |
NNNAGGCATTCAAGAATGCG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
