Construct: sgRNA BRDN0001145988

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGGGCAAGTGTCTCCTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF1L (138474)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77120

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 32632681 - TAF1L NNNGGGCAAGTGTCTCCTAG NGG 0 1.0 Tier I
2 NC_000023.11 X 71392633 + TAF1 NNNGGGCAAGTGTCTGCTAG NGG 1 0.1538 Tier I
3 NC_000010.11 10 18002244 - SLC39A12 NNNGGGCAAGCGTTTCCTAG NGG 2 0.4889 Tier II
4 NC_000019.10 19 17083533 + MYO9B NNNGGCCAAGTGTCTCCTGG NGG 2 0.2557 Tier II
5 NC_000022.11 22 46526624 - CELSR1 NNNGGGCGAGTGTCTCCGAG NGG 2 0.2444 Tier II
6 NC_000001.11 1 44339800 - ERI3 NNNGGGCAAATGGCTCCTAG NGG 2 0.2435 Tier II
7 NC_000003.12 3 183151308 + LAMP3 NNNGGGCAAGTCTCTCCCAG NGG 2 0.2269 Tier II
8 NC_000008.11 8 103206743 + BAALC NNNGTGCAAGTGACTCCTAG NGG 2 0.2077 Tier II
9 NC_000017.11 17 80029438 - TBC1D16 NNNGGCCAAGTGGCTCCTAG NGG 2 0.1779 Tier II
10 NC_000018.10 18 28115498 + CDH2 NNNGGGCAACTGTCTCCTAC NGG 2 0.1714 Tier II
11 NC_000023.11 X 153960995 - HCFC1 NNNGTGCAAGTGTATCCTAG NGG 2 0.105 Tier II
12 NC_000020.11 20 59630042 + PHACTR3 NNNGGGCAAGTGTGTCCTGG NGG 2 0.0 Tier II
13 NC_000010.11 10 18002244 - SLC39A12-AS1 NNNGGGCAAGCGTTTCCTAG NGG 2 0.4889 Tier III
14 NC_000002.12 2 15717616 + LINC01804 NNNGGGCAAGTGTCTCCCAT NGG 2 0.3 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 38281306 - Pcdh12 NNNGGCAAAGTGTCTCCTAG NGG 2 0.5114 Tier I
2 NC_000086.7 X 101556586 + Taf1 NNNGGGCAAATGTCTCCTGG NGG 2 0.35 Tier I
3 NC_000078.6 12 71840413 + Daam1 NNNAGCCAAGTGTCTCCTAG NGG 2 0.6136 Tier II
4 NC_000084.6 18 38281306 - Rnf14 NNNGGCAAAGTGTCTCCTAG NGG 2 0.5114 Tier II
5 NC_000070.6 4 85863930 - Adamtsl1 NNNGGGCAAGTCTCTCCTAT NGG 2 0.3706 Tier II
6 NC_000073.6 7 127485291 + Fbrs NNNGGGAAAGTGTCTCCTAC NGG 2 0.3214 Tier II
7 NC_000070.6 4 137724011 - Rap1gap NNNGGGCCAGAGTCTCCTAG NGG 2 0.3214 Tier II
8 NC_000070.6 4 36014936 + Lingo2 NNNGGGCAAGTGTTTCCCAG NGG 2 0.3143 Tier II
9 NC_000071.6 5 123702257 - Zcchc8 NNNGGGCAGGTGTCTCCCAG NGG 2 0.2857 Tier II
10 NC_000074.6 8 121786523 - Jph3 NNNGGGCAAGTGACTCCTGG NGG 2 0.2596 Tier II
11 NC_000080.6 14 65390239 + Zfp395 NNNGGGCAGGTGTCTCCTAG NAG 2 0.1728 Tier II
12 NC_000076.6 10 76293053 + Dip2a NNNGGGCAAGTGTCTCCTAA NGA 2 0.0651 Tier II
13 NC_000083.6 17 5720993 + Zdhhc14 NNNGGGCATGTGTCTCCTAG NCG 2 0.0643 Tier II
14 NC_000077.6 11 115234004 + Tmem104 NNNGGGCAAGAGTCTCCTAG NGA 2 0.0521 Tier II
15 NC_000068.7 2 27752467 + Rxra NNNGGGCAAGTGGCTGCTAG NGG 2 0.0401 Tier II
16 NC_000076.6 10 127207339 + Pip4k2c NNNGGGCAAGTGTCTCCGAG NGA 2 0.0231 Tier II
17 NC_000068.7 2 29890453 - Odf2 NNNGGGCAACTGTCTCCTAG NTG 2 0.0156 Tier II
18 NC_000081.6 15 89189803 - Dennd6b NNNGGGCAAGTGCCTCCTAG NGT 2 0.0127 Tier II
19 NC_000077.6 11 36662177 - Tenm2 NNNGGCCAAGTGTCTCCTAG NGT 2 0.011 Tier II
20 NC_000077.6 11 33773005 - Kcnip1 NNNGGGCAAGTGTCTCCTTG NGT 2 0.0087 Tier II
21 NC_000083.6 17 14205952 + Gm34510 NNNGGGCAAGTGTCTCCAAA NGG 2 0.625 Tier III
22 NC_000080.6 14 48737198 - Otx2os1 NNNGGGTAAGTGTTTCCTAG NGG 2 0.5958 Tier III
23 NC_000068.7 2 122715963 - 4930417H01Rik NNNGGGCATGTGCCTCCTAG NGG 2 0.4737 Tier III
24 NC_000067.6 1 73724816 - 6030407O03Rik NNNGGTCAAGTGTCTCCTAC NGG 2 0.2857 Tier III
25 NC_000080.6 14 24865766 - Gm32008 NNNGGGCAGGGGTCTCCTAG NGG 2 0.2667 Tier III
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Other clones with same target sequence:

(none)