Construct: sgRNA BRDN0001145991
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTCCTACTAATGCAGCACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GK2 (2712)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77910
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
79407419 |
+ |
GK2 |
NNNCTACTAATGCAGCACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
25085362 |
- |
EFR3B |
NNNCTACAAATGTAGCACAT |
NGG |
2 |
0.56 |
Tier II |
| 3 |
NC_000001.11 |
1 |
28478887 |
- |
PHACTR4 |
NNNCTTGTAATGCAGCACAT |
NGG |
2 |
0.3361 |
Tier II |
| 4 |
NC_000015.10 |
15 |
58486691 |
+ |
LIPC |
NNNCTACCAATGCAGCACAT |
NAG |
2 |
0.2593 |
Tier II |
| 5 |
NC_000005.10 |
5 |
10621847 |
- |
ANKRD33B |
NNNCTTCTAATGCATCACAT |
NGG |
2 |
0.102 |
Tier II |
| 6 |
NC_000004.12 |
4 |
36101433 |
+ |
ARAP2 |
NNNCTACTAATGCAGCAAAT |
NGC |
2 |
0.012 |
Tier II |
| 7 |
NC_000013.11 |
13 |
111250220 |
- |
ARHGEF7 |
NNNCTACTAATGCAGCAAAT |
NGC |
2 |
0.012 |
Tier II |
| 8 |
NC_000007.14 |
7 |
36358712 |
+ |
MATCAP2 |
NNNCTACTAATTCAGCACAT |
NGC |
2 |
0.0085 |
Tier II |
| 9 |
NC_000010.11 |
10 |
45245239 |
+ |
CUBNP2 |
NNNCTACTATTCCAGCACAT |
NGG |
2 |
0.4671 |
Tier III |
| 10 |
NC_000002.12 |
2 |
40780294 |
- |
LOC105374497 |
NNNCTAATTATGCAGCACAT |
NGG |
2 |
0.45 |
Tier III |
| 11 |
NC_000005.10 |
5 |
123054853 |
+ |
PPIC-AS1 |
NNNCTACAAATGAAGCACAT |
NGG |
2 |
0.3077 |
Tier III |
| 12 |
NC_000005.10 |
5 |
174501682 |
- |
LINC01411 |
NNNCTACTAAAGAAGCACAT |
NGG |
2 |
0.2885 |
Tier III |
| 13 |
NC_000015.10 |
15 |
58486691 |
+ |
LIPC-AS1 |
NNNCTACCAATGCAGCACAT |
NAG |
2 |
0.2593 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
44396557 |
+ |
Nudcd1 |
NNNCTGCTATTGCAGCACAT |
NGG |
2 |
0.6303 |
Tier I |
| 2 |
NC_000084.6 |
18 |
80607969 |
- |
Nfatc1 |
NNNCTCCTAATTCAGCACAT |
NGG |
2 |
0.1748 |
Tier I |
| 3 |
NC_000068.7 |
2 |
160790396 |
- |
Zhx3 |
NNNCTACTAATACAGCACAT |
NGG |
1 |
0.9333 |
Tier II |
| 4 |
NC_000081.6 |
15 |
44551807 |
+ |
Pkhd1l1 |
NNNCTATTCATGCAGCACAT |
NGG |
2 |
0.4643 |
Tier II |
| 5 |
NC_000085.6 |
19 |
17555113 |
+ |
Pcsk5 |
NNNCTACTAACCCAGCACAT |
NGG |
2 |
0.3529 |
Tier II |
| 6 |
NC_000069.6 |
3 |
86318563 |
- |
Lrba |
NNNCTACTACTGTAGCACAT |
NGG |
2 |
0.2333 |
Tier II |
| 7 |
NC_000076.6 |
10 |
108185025 |
- |
Ppp1r12a |
NNNCTTCTAATGCAGCACAT |
NAG |
2 |
0.1852 |
Tier II |
| 8 |
NC_000077.6 |
11 |
117855636 |
+ |
Birc5 |
NNNCTGCTAATGCAGCCCAT |
NGG |
2 |
0.1261 |
Tier II |
| 9 |
NC_000073.6 |
7 |
139192611 |
+ |
Stk32c |
NNNCTAGTAATGCAGCACAG |
NGG |
2 |
0.083 |
Tier II |
| 10 |
NC_000074.6 |
8 |
99144807 |
- |
Cdh8 |
NNNCTCCTAATGCAGCACAT |
NGC |
2 |
0.0101 |
Tier II |
| 11 |
NC_000083.6 |
17 |
65841599 |
+ |
Ppp4r1 |
NNNCTCCTAATGCAGCACAT |
NGC |
2 |
0.0101 |
Tier II |
| 12 |
NC_000074.6 |
8 |
99144807 |
- |
Gm15681 |
NNNCTCCTAATGCAGCACAT |
NGC |
2 |
0.0101 |
Tier III |
Other clones with same target sequence:
(none)
