Construct: sgRNA BRDN0001145992

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGGGAAATACAAACTGGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1A1L (122011)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77381

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 37105200 - CSNK1A1L NNNGGAAATACAAACTGGTG NGG 0 1.0 Tier I
2 NC_000005.10 5 149550908 - CSNK1A1 NNNGGAAATATAAACTGGTA NGG 2 0.2885 Tier I
3 NC_000007.14 7 18578579 + HDAC9 NNNGGAAATACAAACTGGTA NGG 1 0.9375 Tier II
4 NC_000012.12 12 103161937 - C12orf42 NNNGGGAAAACAAACTGGTG NGG 2 0.6633 Tier II
5 NC_000015.10 15 71581160 - THSD4 NNNGGAAATGCAAACTGGTA NGG 2 0.5208 Tier II
6 NC_000017.11 17 65897801 - CEP112 NNNGGAAATAAAATCTGGTG NGG 2 0.4 Tier II
7 NC_000013.11 13 92566049 + GPC5 NNNTAAAATACAAACTGGTG NGG 2 0.3152 Tier II
8 NC_000014.9 14 29819299 + PRKD1 NNNGGAAATACATACTGGTG NGG 1 0.3 Tier II
9 NC_000011.10 11 24863116 + LUZP2 NNNGGAAATTTAAACTGGTG NGG 2 0.2715 Tier II
10 NC_000008.11 8 87408913 - CNBD1 NNNGGAATGACAAACTGGTG NGG 2 0.2653 Tier II
11 NC_000004.12 4 17898918 - LCORL NNNGGAAATACAAACTGGTG NAG 1 0.2593 Tier II
12 NC_000023.11 X 153352664 - ZNF275 NNNGGAAATACAAACTGGGG NGG 1 0.25 Tier II
13 NC_000012.12 12 100891421 - ANO4 NNNGGAAATACAAAATGGTA NGG 2 0.2083 Tier II
14 NC_000017.11 17 64071103 + ERN1 NNNGGAAACACAAAATGGTG NGG 2 0.2051 Tier II
15 NC_000012.12 12 96364355 - CDK17 NNNGGGAATACAAAATGGTG NGG 2 0.1587 Tier II
16 NC_000010.11 10 115271667 - ATRNL1 NNNGGAAATACAAAATGGTT NGG 2 0.1556 Tier II
17 NC_000012.12 12 96382117 + CDK17 NNNGGAAATGCAAAATGGTG NGG 2 0.1235 Tier II
18 NC_000010.11 10 73449130 + PPP3CB NNNTGAAATACAAACTTGTG NGG 2 0.0909 Tier II
19 NC_000001.11 1 61870826 - PATJ NNNGGAAATATAAACTGGTG NAG 2 0.0798 Tier II
20 NC_000006.12 6 16584782 + ATXN1 NNNGGAAATACAAACTGGTA NGA 2 0.0651 Tier II
21 NC_000007.14 7 122364844 + CADPS2 NNNGGAAATACAAACTAGTG NGA 2 0.0648 Tier II
22 NC_000001.11 1 174383858 + RABGAP1L NNNGGAAATACAAAATGGTG NAG 2 0.0576 Tier II
23 NC_000005.10 5 56189921 - ANKRD55 NNNGGAAATACAAAATGGTG NAG 2 0.0576 Tier II
24 NC_000005.10 5 140587291 + LOC112267855 NNNGGAAATACAAAATGGTG NAG 2 0.0576 Tier II
25 NC_000011.10 11 88693344 + GRM5 NNNGGAAATACAAAGTAGTG NGG 2 0.0467 Tier II
26 NC_000010.11 10 82532870 - NRG3 NNNGGAAATGCAAAGTGGTG NGG 2 0.0278 Tier II
27 NC_000014.9 14 20475403 + PNP NNNTGAAATACAAACTGGTG NGA 2 0.0253 Tier II
28 NC_000002.12 2 165668309 + CSRNP3 NNNGGAAATACTAACTGGTG NGA 2 0.0231 Tier II
29 NC_000001.11 1 226233904 - LIN9 NNNGGAAATACAAACTGGAG NGA 2 0.0198 Tier II
30 NC_000007.14 7 95478304 + ASB4 NNNGGAAATACAAGCTGGTG NTG 2 0.0182 Tier II
31 NC_000005.10 5 156233501 + SGCD NNNGGAAATACAAAGTGGAG NGG 2 0.0143 Tier II
32 NC_000009.12 9 112063380 + SUSD1 NNNGGAAATACACAGTGGTG NGG 2 0.0105 Tier II
33 NC_000007.14 7 15279592 + AGMO NNNGGAAAGACAAACTGGTG NGT 2 0.01 Tier II
34 NC_000002.12 2 167983536 + STK39 NNNGGAAATAGAAACTGGTG NTG 2 0.0097 Tier II
35 NC_000012.12 12 103161937 - C12orf42-AS1 NNNGGGAAAACAAACTGGTG NGG 2 0.6633 Tier III
36 NC_000014.9 14 48956422 - LOC105378178 NNNGGAAATGCAAACTGGTA NGG 2 0.5208 Tier III
37 NC_000005.10 5 109238303 - LOC285638 NNNGGAAAGACAAACTGATG NGG 2 0.4286 Tier III
38 NC_000017.11 17 65897801 - LOC105371867 NNNGGAAATAAAATCTGGTG NGG 2 0.4 Tier III
39 NC_000005.10 5 152296300 - LOC105378236 NNNGGAAATAAAAACTGCTG NGG 2 0.3571 Tier III
40 NC_000012.12 12 5264873 + LOC105369617 NNNTAAAATACAAACTGGTG NGG 2 0.3152 Tier III
41 NC_000023.11 X 153352664 - LOC105373378 NNNGGAAATACAAACTGGGG NGG 1 0.25 Tier III
42 NC_000014.9 14 22474220 - TRA NNNGGAAATACAAAATGGTG NGG 1 0.2222 Tier III
43 NC_000014.9 14 22474220 - TRD-AS1 NNNGGAAATACAAAATGGTG NGG 1 0.2222 Tier III
44 NC_000011.10 11 25691450 - LINC02699 NNNGGAAATATAAACTGGTT NGG 2 0.2154 Tier III
45 NC_000022.11 22 28843150 + LOC107985549 NNNAGAAATACAAAATGGTG NGG 2 0.2 Tier III
46 NC_000008.11 8 126663226 + LOC105375751 NNNGGAAATACAAAATGGTG NAG 2 0.0576 Tier III
47 NC_000014.9 14 31531579 - LOC105370438 NNNGGAAATACAAAATGGTG NAG 2 0.0576 Tier III
48 NC_000007.14 7 15279592 + LOC124901592 NNNGGAAAGACAAACTGGTG NGT 2 0.01 Tier III
49 NC_000002.12 2 217629843 - DIRC3 NNNGGAAATACAAACTTGTG NGT 2 0.004 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 61555808 + Csnk1a1 NNNGAAAATACAAACTGGTG NGG 1 0.8667 Tier I
2 NC_000079.6 13 49901073 + Gm8674 NNNGGAAAAACAAACTGGTG NAG 2 0.2407 Tier I
3 NC_000070.6 4 140732633 - Padi6 NNNTGAAATACACACTGGTG NGG 2 0.0766 Tier I
4 NC_000068.7 2 11252565 - Prkcq NNNGGAAAATCAAACTGGTG NGG 2 0.8193 Tier II
5 NC_000067.6 1 57832233 + Spats2l NNNGGAAATAAAAACTGGTA NGG 2 0.7031 Tier II
6 NC_000077.6 11 108252139 + Prkca NNNAGAAATACAAACTGGTT NGG 2 0.63 Tier II
7 NC_000067.6 1 181959571 - Enah NNNAGAAATACAATCTGGTG NGG 2 0.48 Tier II
8 NC_000072.6 6 38510831 - Ubn2 NNNGAAAATACAATCTGGTG NGG 2 0.4622 Tier II
9 NC_000073.6 7 34119772 + Wtip NNNGGAACTACAAACTGGTG NGG 1 0.4286 Tier II
10 NC_000069.6 3 86238379 + Lrba NNNTGAAATACAAACAGGTG NGG 2 0.3306 Tier II
11 NC_000074.6 8 81494835 - Inpp4b NNNGGAAATCCAAACTAGTG NGG 2 0.3111 Tier II
12 NC_000070.6 4 41245567 - Ubap2 NNNGGAATTACAAACTGTTG NGG 2 0.2857 Tier II
13 NC_000068.7 2 25730293 - Obp2b NNNAGAAATACAAACTGGAG NGG 2 0.2571 Tier II
14 NC_000070.6 4 94856516 + Tek NNNGGAAATAATAACTGGTG NGG 2 0.25 Tier II
15 NC_000070.6 4 135220040 + Clic4 NNNGGAAATACAAACTGGTA NAG 2 0.2431 Tier II
16 NC_000079.6 13 58204511 - Ubqln1 NNNGTAAGTACAAACTGGTG NGG 2 0.22 Tier II
17 NC_000067.6 1 136889624 - Nr5a2 NNNGGTAATATAAACTGGTG NGG 2 0.2198 Tier II
18 NC_000082.6 16 40500025 - Lsamp NNNGGAAACACAAAATGGTG NGG 2 0.2051 Tier II
19 NC_000082.6 16 6432415 + Rbfox1 NNNGGAAATAACAACTGGTG NGG 2 0.1974 Tier II
20 NC_000074.6 8 109523282 - Pmfbp1 NNNGAAAATACAAAATGGTG NGG 2 0.1926 Tier II
21 NC_000082.6 16 17506006 + Aifm3 NNNGGAAAGACAAACTGGTG NAG 2 0.1605 Tier II
22 NC_000072.6 6 37356607 - Creb3l2 NNNGGAAAGAGAAACTGGTG NGG 2 0.1548 Tier II
23 NC_000071.6 5 5046966 + Cdk14 NNNGGAAATGCAAACTGGTG NAG 2 0.144 Tier II
24 NC_000077.6 11 21883774 + Wdpcp NNNGGAAATGCAAACTGGTG NAG 2 0.144 Tier II
25 NC_000086.7 X 38014675 - Btg1b NNNGGAACTACAAACTCGTG NGG 2 0.1008 Tier II
26 NC_000086.7 X 38039121 - Btg1c NNNGGAACTACAAACTCGTG NGG 2 0.1008 Tier II
27 NC_000068.7 2 91266486 + Arfgap2 NNNTGAAATACAAACTGGGG NGG 2 0.0909 Tier II
28 NC_000070.6 4 62979119 + Zfp618 NNNAGAAATACAAATTGGTG NGG 2 0.06 Tier II
29 NC_000070.6 4 151800447 + Camta1 NNNGGCAATACAAACTGGTG NCG 2 0.0487 Tier II
30 NC_000076.6 10 84957997 - Ric8b NNNGGAAATACAAAGTGGAG NGG 2 0.0143 Tier II
31 NC_000077.6 11 24215903 + Gm12066 NNNGGAAATACAATCTGATG NGG 2 0.3692 Tier III
32 NC_000067.6 1 193730736 + Gm31870 NNNGGAAATACAAACTGGGG NGG 1 0.25 Tier III
33 NC_000068.7 2 35498865 + Gm35260 NNNGGAAATACACACAGGTG NGG 2 0.1914 Tier III
34 NC_000077.6 11 40052283 - Gm12132 NNNAGAAATACACACTGGTG NGG 2 0.1895 Tier III
35 NC_000070.6 4 62979119 + Gm30764 NNNAGAAATACAAATTGGTG NGG 2 0.06 Tier III
36 NC_000075.6 9 9616219 + Gm46102 NNNGGTAATACAAACTGGTG NTG 2 0.0278 Tier III
37 NC_000075.6 9 115518432 - Gm39447 NNNGGAAATACAAAGTGCTG NGG 2 0.0238 Tier III
38 NC_000075.6 9 83044057 + Gm39383 NNNGGAAATACACACTGGTG NTG 2 0.0082 Tier III
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Other clones with same target sequence:

(none)