Construct: sgRNA BRDN0001145992
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGGGAAATACAAACTGGTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- CSNK1A1L (122011)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000013.11 | 13 | 37105200 | - | CSNK1A1L | NNNGGAAATACAAACTGGTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000005.10 | 5 | 149550908 | - | CSNK1A1 | NNNGGAAATATAAACTGGTA | NGG | 2 | 0.2885 | Tier I |
3 | NC_000007.14 | 7 | 18578579 | + | HDAC9 | NNNGGAAATACAAACTGGTA | NGG | 1 | 0.9375 | Tier II |
4 | NC_000012.12 | 12 | 103161937 | - | C12orf42 | NNNGGGAAAACAAACTGGTG | NGG | 2 | 0.6633 | Tier II |
5 | NC_000015.10 | 15 | 71581160 | - | THSD4 | NNNGGAAATGCAAACTGGTA | NGG | 2 | 0.5208 | Tier II |
6 | NC_000017.11 | 17 | 65897801 | - | CEP112 | NNNGGAAATAAAATCTGGTG | NGG | 2 | 0.4 | Tier II |
7 | NC_000013.11 | 13 | 92566049 | + | GPC5 | NNNTAAAATACAAACTGGTG | NGG | 2 | 0.3152 | Tier II |
8 | NC_000014.9 | 14 | 29819299 | + | PRKD1 | NNNGGAAATACATACTGGTG | NGG | 1 | 0.3 | Tier II |
9 | NC_000011.10 | 11 | 24863116 | + | LUZP2 | NNNGGAAATTTAAACTGGTG | NGG | 2 | 0.2715 | Tier II |
10 | NC_000008.11 | 8 | 87408913 | - | CNBD1 | NNNGGAATGACAAACTGGTG | NGG | 2 | 0.2653 | Tier II |
11 | NC_000004.12 | 4 | 17898918 | - | LCORL | NNNGGAAATACAAACTGGTG | NAG | 1 | 0.2593 | Tier II |
12 | NC_000023.11 | X | 153352664 | - | ZNF275 | NNNGGAAATACAAACTGGGG | NGG | 1 | 0.25 | Tier II |
13 | NC_000012.12 | 12 | 100891421 | - | ANO4 | NNNGGAAATACAAAATGGTA | NGG | 2 | 0.2083 | Tier II |
14 | NC_000017.11 | 17 | 64071103 | + | ERN1 | NNNGGAAACACAAAATGGTG | NGG | 2 | 0.2051 | Tier II |
15 | NC_000012.12 | 12 | 96364355 | - | CDK17 | NNNGGGAATACAAAATGGTG | NGG | 2 | 0.1587 | Tier II |
16 | NC_000010.11 | 10 | 115271667 | - | ATRNL1 | NNNGGAAATACAAAATGGTT | NGG | 2 | 0.1556 | Tier II |
17 | NC_000012.12 | 12 | 96382117 | + | CDK17 | NNNGGAAATGCAAAATGGTG | NGG | 2 | 0.1235 | Tier II |
18 | NC_000010.11 | 10 | 73449130 | + | PPP3CB | NNNTGAAATACAAACTTGTG | NGG | 2 | 0.0909 | Tier II |
19 | NC_000001.11 | 1 | 61870826 | - | PATJ | NNNGGAAATATAAACTGGTG | NAG | 2 | 0.0798 | Tier II |
20 | NC_000006.12 | 6 | 16584782 | + | ATXN1 | NNNGGAAATACAAACTGGTA | NGA | 2 | 0.0651 | Tier II |
21 | NC_000007.14 | 7 | 122364844 | + | CADPS2 | NNNGGAAATACAAACTAGTG | NGA | 2 | 0.0648 | Tier II |
22 | NC_000001.11 | 1 | 174383858 | + | RABGAP1L | NNNGGAAATACAAAATGGTG | NAG | 2 | 0.0576 | Tier II |
23 | NC_000005.10 | 5 | 56189921 | - | ANKRD55 | NNNGGAAATACAAAATGGTG | NAG | 2 | 0.0576 | Tier II |
24 | NC_000005.10 | 5 | 140587291 | + | LOC112267855 | NNNGGAAATACAAAATGGTG | NAG | 2 | 0.0576 | Tier II |
25 | NC_000011.10 | 11 | 88693344 | + | GRM5 | NNNGGAAATACAAAGTAGTG | NGG | 2 | 0.0467 | Tier II |
26 | NC_000010.11 | 10 | 82532870 | - | NRG3 | NNNGGAAATGCAAAGTGGTG | NGG | 2 | 0.0278 | Tier II |
27 | NC_000014.9 | 14 | 20475403 | + | PNP | NNNTGAAATACAAACTGGTG | NGA | 2 | 0.0253 | Tier II |
28 | NC_000002.12 | 2 | 165668309 | + | CSRNP3 | NNNGGAAATACTAACTGGTG | NGA | 2 | 0.0231 | Tier II |
29 | NC_000001.11 | 1 | 226233904 | - | LIN9 | NNNGGAAATACAAACTGGAG | NGA | 2 | 0.0198 | Tier II |
30 | NC_000007.14 | 7 | 95478304 | + | ASB4 | NNNGGAAATACAAGCTGGTG | NTG | 2 | 0.0182 | Tier II |
31 | NC_000005.10 | 5 | 156233501 | + | SGCD | NNNGGAAATACAAAGTGGAG | NGG | 2 | 0.0143 | Tier II |
32 | NC_000009.12 | 9 | 112063380 | + | SUSD1 | NNNGGAAATACACAGTGGTG | NGG | 2 | 0.0105 | Tier II |
33 | NC_000007.14 | 7 | 15279592 | + | AGMO | NNNGGAAAGACAAACTGGTG | NGT | 2 | 0.01 | Tier II |
34 | NC_000002.12 | 2 | 167983536 | + | STK39 | NNNGGAAATAGAAACTGGTG | NTG | 2 | 0.0097 | Tier II |
35 | NC_000012.12 | 12 | 103161937 | - | C12orf42-AS1 | NNNGGGAAAACAAACTGGTG | NGG | 2 | 0.6633 | Tier III |
36 | NC_000014.9 | 14 | 48956422 | - | LOC105378178 | NNNGGAAATGCAAACTGGTA | NGG | 2 | 0.5208 | Tier III |
37 | NC_000005.10 | 5 | 109238303 | - | LOC285638 | NNNGGAAAGACAAACTGATG | NGG | 2 | 0.4286 | Tier III |
38 | NC_000017.11 | 17 | 65897801 | - | LOC105371867 | NNNGGAAATAAAATCTGGTG | NGG | 2 | 0.4 | Tier III |
39 | NC_000005.10 | 5 | 152296300 | - | LOC105378236 | NNNGGAAATAAAAACTGCTG | NGG | 2 | 0.3571 | Tier III |
40 | NC_000012.12 | 12 | 5264873 | + | LOC105369617 | NNNTAAAATACAAACTGGTG | NGG | 2 | 0.3152 | Tier III |
41 | NC_000023.11 | X | 153352664 | - | LOC105373378 | NNNGGAAATACAAACTGGGG | NGG | 1 | 0.25 | Tier III |
42 | NC_000014.9 | 14 | 22474220 | - | TRA | NNNGGAAATACAAAATGGTG | NGG | 1 | 0.2222 | Tier III |
43 | NC_000014.9 | 14 | 22474220 | - | TRD-AS1 | NNNGGAAATACAAAATGGTG | NGG | 1 | 0.2222 | Tier III |
44 | NC_000011.10 | 11 | 25691450 | - | LINC02699 | NNNGGAAATATAAACTGGTT | NGG | 2 | 0.2154 | Tier III |
45 | NC_000022.11 | 22 | 28843150 | + | LOC107985549 | NNNAGAAATACAAAATGGTG | NGG | 2 | 0.2 | Tier III |
46 | NC_000008.11 | 8 | 126663226 | + | LOC105375751 | NNNGGAAATACAAAATGGTG | NAG | 2 | 0.0576 | Tier III |
47 | NC_000014.9 | 14 | 31531579 | - | LOC105370438 | NNNGGAAATACAAAATGGTG | NAG | 2 | 0.0576 | Tier III |
48 | NC_000007.14 | 7 | 15279592 | + | LOC124901592 | NNNGGAAAGACAAACTGGTG | NGT | 2 | 0.01 | Tier III |
49 | NC_000002.12 | 2 | 217629843 | - | DIRC3 | NNNGGAAATACAAACTTGTG | NGT | 2 | 0.004 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000084.6 | 18 | 61555808 | + | Csnk1a1 | NNNGAAAATACAAACTGGTG | NGG | 1 | 0.8667 | Tier I |
2 | NC_000079.6 | 13 | 49901073 | + | Gm8674 | NNNGGAAAAACAAACTGGTG | NAG | 2 | 0.2407 | Tier I |
3 | NC_000070.6 | 4 | 140732633 | - | Padi6 | NNNTGAAATACACACTGGTG | NGG | 2 | 0.0766 | Tier I |
4 | NC_000068.7 | 2 | 11252565 | - | Prkcq | NNNGGAAAATCAAACTGGTG | NGG | 2 | 0.8193 | Tier II |
5 | NC_000067.6 | 1 | 57832233 | + | Spats2l | NNNGGAAATAAAAACTGGTA | NGG | 2 | 0.7031 | Tier II |
6 | NC_000077.6 | 11 | 108252139 | + | Prkca | NNNAGAAATACAAACTGGTT | NGG | 2 | 0.63 | Tier II |
7 | NC_000067.6 | 1 | 181959571 | - | Enah | NNNAGAAATACAATCTGGTG | NGG | 2 | 0.48 | Tier II |
8 | NC_000072.6 | 6 | 38510831 | - | Ubn2 | NNNGAAAATACAATCTGGTG | NGG | 2 | 0.4622 | Tier II |
9 | NC_000073.6 | 7 | 34119772 | + | Wtip | NNNGGAACTACAAACTGGTG | NGG | 1 | 0.4286 | Tier II |
10 | NC_000069.6 | 3 | 86238379 | + | Lrba | NNNTGAAATACAAACAGGTG | NGG | 2 | 0.3306 | Tier II |
11 | NC_000074.6 | 8 | 81494835 | - | Inpp4b | NNNGGAAATCCAAACTAGTG | NGG | 2 | 0.3111 | Tier II |
12 | NC_000070.6 | 4 | 41245567 | - | Ubap2 | NNNGGAATTACAAACTGTTG | NGG | 2 | 0.2857 | Tier II |
13 | NC_000068.7 | 2 | 25730293 | - | Obp2b | NNNAGAAATACAAACTGGAG | NGG | 2 | 0.2571 | Tier II |
14 | NC_000070.6 | 4 | 94856516 | + | Tek | NNNGGAAATAATAACTGGTG | NGG | 2 | 0.25 | Tier II |
15 | NC_000070.6 | 4 | 135220040 | + | Clic4 | NNNGGAAATACAAACTGGTA | NAG | 2 | 0.2431 | Tier II |
16 | NC_000079.6 | 13 | 58204511 | - | Ubqln1 | NNNGTAAGTACAAACTGGTG | NGG | 2 | 0.22 | Tier II |
17 | NC_000067.6 | 1 | 136889624 | - | Nr5a2 | NNNGGTAATATAAACTGGTG | NGG | 2 | 0.2198 | Tier II |
18 | NC_000082.6 | 16 | 40500025 | - | Lsamp | NNNGGAAACACAAAATGGTG | NGG | 2 | 0.2051 | Tier II |
19 | NC_000082.6 | 16 | 6432415 | + | Rbfox1 | NNNGGAAATAACAACTGGTG | NGG | 2 | 0.1974 | Tier II |
20 | NC_000074.6 | 8 | 109523282 | - | Pmfbp1 | NNNGAAAATACAAAATGGTG | NGG | 2 | 0.1926 | Tier II |
21 | NC_000082.6 | 16 | 17506006 | + | Aifm3 | NNNGGAAAGACAAACTGGTG | NAG | 2 | 0.1605 | Tier II |
22 | NC_000072.6 | 6 | 37356607 | - | Creb3l2 | NNNGGAAAGAGAAACTGGTG | NGG | 2 | 0.1548 | Tier II |
23 | NC_000071.6 | 5 | 5046966 | + | Cdk14 | NNNGGAAATGCAAACTGGTG | NAG | 2 | 0.144 | Tier II |
24 | NC_000077.6 | 11 | 21883774 | + | Wdpcp | NNNGGAAATGCAAACTGGTG | NAG | 2 | 0.144 | Tier II |
25 | NC_000086.7 | X | 38014675 | - | Btg1b | NNNGGAACTACAAACTCGTG | NGG | 2 | 0.1008 | Tier II |
26 | NC_000086.7 | X | 38039121 | - | Btg1c | NNNGGAACTACAAACTCGTG | NGG | 2 | 0.1008 | Tier II |
27 | NC_000068.7 | 2 | 91266486 | + | Arfgap2 | NNNTGAAATACAAACTGGGG | NGG | 2 | 0.0909 | Tier II |
28 | NC_000070.6 | 4 | 62979119 | + | Zfp618 | NNNAGAAATACAAATTGGTG | NGG | 2 | 0.06 | Tier II |
29 | NC_000070.6 | 4 | 151800447 | + | Camta1 | NNNGGCAATACAAACTGGTG | NCG | 2 | 0.0487 | Tier II |
30 | NC_000076.6 | 10 | 84957997 | - | Ric8b | NNNGGAAATACAAAGTGGAG | NGG | 2 | 0.0143 | Tier II |
31 | NC_000077.6 | 11 | 24215903 | + | Gm12066 | NNNGGAAATACAATCTGATG | NGG | 2 | 0.3692 | Tier III |
32 | NC_000067.6 | 1 | 193730736 | + | Gm31870 | NNNGGAAATACAAACTGGGG | NGG | 1 | 0.25 | Tier III |
33 | NC_000068.7 | 2 | 35498865 | + | Gm35260 | NNNGGAAATACACACAGGTG | NGG | 2 | 0.1914 | Tier III |
34 | NC_000077.6 | 11 | 40052283 | - | Gm12132 | NNNAGAAATACACACTGGTG | NGG | 2 | 0.1895 | Tier III |
35 | NC_000070.6 | 4 | 62979119 | + | Gm30764 | NNNAGAAATACAAATTGGTG | NGG | 2 | 0.06 | Tier III |
36 | NC_000075.6 | 9 | 9616219 | + | Gm46102 | NNNGGTAATACAAACTGGTG | NTG | 2 | 0.0278 | Tier III |
37 | NC_000075.6 | 9 | 115518432 | - | Gm39447 | NNNGGAAATACAAAGTGCTG | NGG | 2 | 0.0238 | Tier III |
38 | NC_000075.6 | 9 | 83044057 | + | Gm39383 | NNNGGAAATACACACTGGTG | NTG | 2 | 0.0082 | Tier III |