Construct: sgRNA BRDN0001145999
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGCCGTGCATAGACTGCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CKMT1A (548596)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75878
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
43595031 |
- |
CKMT1B |
NNNCCGTGCATAGACTGCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
43694863 |
- |
CKMT1A |
NNNCCGTGCATAGACTGCTG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000006.12 |
6 |
35295867 |
+ |
ZNF76 |
NNNCTGTACATAGACTGCTG |
NGG |
2 |
0.6364 |
Tier II |
| 4 |
NC_000003.12 |
3 |
37250497 |
- |
GOLGA4 |
NNNCCGTGCACAAACTGCTG |
NGG |
2 |
0.6154 |
Tier II |
| 5 |
NC_000005.10 |
5 |
179272335 |
+ |
ADAMTS2 |
NNNCTGTGCAGAGACTGCTG |
NGG |
2 |
0.2545 |
Tier II |
| 6 |
NC_000013.11 |
13 |
114022032 |
- |
RASA3 |
NNNCCGTGCACAGACTGCTG |
NTG |
2 |
0.026 |
Tier II |
| 7 |
NC_000007.14 |
7 |
24142138 |
- |
LOC107986777 |
NNNCCGTGCATACACTGCTT |
NGG |
2 |
0.2947 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
121359638 |
- |
Ckmt1 |
NNNCCGTGCATAGACTGCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000086.7 |
X |
7676139 |
- |
Magix |
NNNCCGTGCATGGACTGCTG |
NAG |
2 |
0.1872 |
Tier I |
| 3 |
NC_000069.6 |
3 |
95740219 |
- |
Tars2 |
NNNCCGTGCAGAGACTGCTG |
NAG |
2 |
0.1037 |
Tier I |
| 4 |
NC_000082.6 |
16 |
94250600 |
- |
Hlcs |
NNNCCGTGCATAAACTGCTG |
NGG |
1 |
0.9231 |
Tier II |
| 5 |
NC_000074.6 |
8 |
95025844 |
+ |
Adgrg3 |
NNNCCCTGCATAAACTGCTG |
NGG |
2 |
0.6294 |
Tier II |
| 6 |
NC_000076.6 |
10 |
13773927 |
+ |
Aig1 |
NNNCTGTGTATAGACTGCTG |
NGG |
2 |
0.5568 |
Tier II |
| 7 |
NC_000077.6 |
11 |
62061497 |
+ |
Specc1 |
NNNCACTGCATAGACTGCTG |
NGG |
2 |
0.3896 |
Tier II |
| 8 |
NC_000067.6 |
1 |
45819371 |
- |
Wdr75 |
NNNCCCTGCGTAGACTGCTG |
NGG |
2 |
0.3788 |
Tier II |
| 9 |
NC_000077.6 |
11 |
6026661 |
- |
Camk2b |
NNNCCTTGCATCGACTGCTG |
NGG |
2 |
0.1754 |
Tier II |
| 10 |
NC_000068.7 |
2 |
4784941 |
+ |
Bend7 |
NNNCCCTGCATAGCCTGCTG |
NGG |
2 |
0.1461 |
Tier II |
| 11 |
NC_000073.6 |
7 |
13261993 |
- |
Zswim9 |
NNNCCTTGCATAGACTGCTG |
NTG |
2 |
0.026 |
Tier II |
Other clones with same target sequence:
(none)
