Construct: sgRNA BRDN0001146001
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTTCTACTTTCGAAGTTAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80243
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
148969124 |
- |
UST |
NNNTATACTTTCAAAGTTAA |
NGG |
2 |
0.5275 |
Tier II |
| 2 |
NC_000012.12 |
12 |
25525533 |
- |
LMNTD1 |
NNNTCAACTTTCGAAGTCAA |
NGG |
2 |
0.3714 |
Tier II |
| 3 |
NC_000015.10 |
15 |
86362301 |
- |
AGBL1 |
NNNTCTACTATCGAAGTTAA |
NAG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000002.12 |
2 |
226793980 |
+ |
IRS1 |
NNNTCTACTTTTGAACTTAA |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000008.11 |
8 |
19311701 |
+ |
LOC105379306 |
NNNTCTACTTTTGAAATTAA |
NGG |
2 |
0.5385 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
64795699 |
+ |
Klhl2 |
NNNTCTATTCTCGAAGTTAA |
NGG |
2 |
0.4667 |
Tier II |
| 2 |
NC_000070.6 |
4 |
82985328 |
- |
Frem1 |
NNNTCTACTTTTTAAGTTAA |
NGG |
2 |
0.1615 |
Tier II |
| 3 |
NC_000068.7 |
2 |
180967783 |
- |
Arfgap1 |
NNNTCTTCTTTCGAAGTGAA |
NGG |
2 |
0.1458 |
Tier II |
| 4 |
NC_000078.6 |
12 |
85111368 |
+ |
Dlst |
NNNTCTACTTTCGGAGTTAA |
NAG |
2 |
0.121 |
Tier II |
| 5 |
NC_000075.6 |
9 |
79674933 |
- |
Col12a1 |
NNNTCTACTTTCCATGTTAA |
NGG |
2 |
0.0842 |
Tier II |
| 6 |
NC_000082.6 |
16 |
43451697 |
- |
Zbtb20 |
NNNTCTACTTTAGAAGGTAA |
NGG |
2 |
0.084 |
Tier II |
| 7 |
NC_000076.6 |
10 |
64027186 |
+ |
Lrrtm3 |
NNNTCTACTTTCTAAGTTAA |
NGC |
2 |
0.0067 |
Tier II |
| 8 |
NC_000076.6 |
10 |
64027186 |
+ |
Ctnna3 |
NNNTCTACTTTCTAAGTTAA |
NGC |
2 |
0.0067 |
Tier II |
| 9 |
NC_000074.6 |
8 |
25104724 |
+ |
5830408C22Rik |
NNNTCTATTTTCGAAGCTAA |
NGG |
2 |
0.6176 |
Tier III |
| 10 |
NC_000074.6 |
8 |
64795699 |
+ |
Gm46040 |
NNNTCTATTCTCGAAGTTAA |
NGG |
2 |
0.4667 |
Tier III |
Other clones with same target sequence:
(none)
