Construct: sgRNA BRDN0001146002

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTATGTCAGGTTATTCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK9 (5601)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76719

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 180261776 - MAPK9 NNNATGTCAGGTTATTCACA NGG 0 1.0 Tier I
2 NC_000018.10 18 28002568 + CDH2 NNNATGTCAGACTATTCACA NGG 2 0.9474 Tier II
3 NC_000019.10 19 10141954 - DNMT1 NNNATGTCGGGTTATACACA NGG 2 0.6061 Tier II
4 NC_000019.10 19 37343626 + ZNF875 NNNAGCTCAGGTTATTCACA NGG 2 0.4364 Tier II
5 NC_000008.11 8 96792122 + CPQ NNNATGTTAGTTTATTCACA NGG 2 0.3365 Tier II
6 NC_000001.11 1 149005543 + PDE4DIP NNNATGTCTGGGTATTCACA NGG 2 0.3 Tier II
7 NC_000002.12 2 36879448 - STRN NNNATTTCAGGTTATTCACA NAG 2 0.1728 Tier II
8 NC_000015.10 15 76888944 + SCAPER NNNAGGTCAGGTTATTCACA NAG 2 0.1659 Tier II
9 NC_000007.14 7 29209855 + CHN2 NNNATTTCAGGTTATTCCCA NGG 2 0.127 Tier II
10 NC_000002.12 2 54587872 - SPTBN1 NNNATGTCAGGTCAGTCACA NGG 2 0.0395 Tier II
11 NC_000008.11 8 101616512 - GRHL2 NNNATGTCAGGTGATTCACA NGA 2 0.0181 Tier II
12 NC_000007.14 7 49807499 - VWC2 NNNATGTCAGGTTAGTCACA NAG 2 0.013 Tier II
13 NC_000023.11 X 29028070 - IL1RAPL1 NNNATGTCAGGTAATTCACA NGT 2 0.0112 Tier II
14 NC_000002.12 2 43360525 + THADA NNNATGTCAGGTTATTTACA NGT 2 0.0075 Tier II
15 NC_000015.10 15 36348886 + LOC105370767 NNNATGTCAGATTATTAACA NGG 2 0.4667 Tier III
16 NC_000008.11 8 96792122 + LOC124901985 NNNATGTTAGTTTATTCACA NGG 2 0.3365 Tier III
17 NC_000001.11 1 16550596 - PDE4DIPP8 NNNATGTCTGGGTATTCACA NGG 2 0.3 Tier III
18 NC_000001.11 1 16857346 + PDE4DIPP9 NNNATGTCTGGGTATTCACA NGG 2 0.3 Tier III
19 NC_000006.12 6 138770508 - CCDC28A-AS1 NNNATCTCAGGTGATTCACA NGG 2 0.1779 Tier III
20 NC_000003.12 3 976975 + LOC107986059 NNNATGTCTGGTTACTCACA NGG 2 0.1636 Tier III
21 NC_000014.9 14 80388463 - DIO2-AS1 NNNATCTCAGGTTATTCACC NGG 2 0.155 Tier III
22 NC_000002.12 2 12485731 - MIR3681HG NNNATGTCAGGGTATTCACA NAG 2 0.1296 Tier III
23 NC_000002.12 2 175662187 + LOC107985962 NNNATGTCAGGTTATTCTCC NGG 2 0.1136 Tier III
24 NC_000002.12 2 54587872 - LOC124906008 NNNATGTCAGGTCAGTCACA NGG 2 0.0395 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 49869219 + Mapk9 NNNATGTCAGGTTATTCATA NGG 1 0.4615 Tier I
2 NC_000075.6 9 105259065 - Nek11 NNNATGTTAGGTTATACACA NGG 2 0.7955 Tier II
3 NC_000075.6 9 59410790 + Arih1 NNNATGTGAGATTATTCACA NGG 2 0.6429 Tier II
4 NC_000081.6 15 31269236 - Dap NNNATCTCAGGATATTCACA NGG 2 0.5455 Tier II
5 NC_000067.6 1 126184450 + Nckap5 NNNATATCAGGTTATTCAAA NGG 2 0.4286 Tier II
6 NC_000068.7 2 69007010 - Cers6 NNNATGTCAGGATTTTCACA NGG 2 0.4267 Tier II
7 NC_000072.6 6 28301833 + Zfp800 NNNATGTCATGGTATTCACA NGG 2 0.4063 Tier II
8 NC_000082.6 16 74726841 + Robo2 NNNATGTCATGTTATTTACA NGG 2 0.3792 Tier II
9 NC_000067.6 1 21456889 + Kcnq5 NNNATTTCAGGTTATTCTCA NGG 2 0.3333 Tier II
10 NC_000077.6 11 52769767 - Fstl4 NNNATGTCATTTTATTCACA NGG 2 0.3125 Tier II
11 NC_000067.6 1 191732842 + Lpgat1 NNNATGTCAGGGTAATCACA NGG 2 0.2895 Tier II
12 NC_000074.6 8 36237325 - Lonrf1 NNNATGTCTGGTTATTCAAA NGG 2 0.2571 Tier II
13 NC_000085.6 19 6073805 + Vps51 NNNATGTCATGTTACTCACA NGG 2 0.2216 Tier II
14 NC_000074.6 8 104172258 - Bean1 NNNATGTCAGGAGATTCACA NGG 2 0.2087 Tier II
15 NC_000086.7 X 162524304 - Reps2 NNNATGTCAGGTGTTTCACA NGG 2 0.1391 Tier II
16 NC_000081.6 15 30561852 + Ctnnd2 NNNATGCCAGGTTATTGACA NGG 2 0.0404 Tier II
17 NC_000081.6 15 36559989 - Snx31 NNNATCTCAGGTTATTGACA NGG 2 0.0401 Tier II
18 NC_000079.6 13 9579054 - Dip2c NNNATGTCAGGTTATTCAGA NAG 2 0.0324 Tier II
19 NC_000067.6 1 100332485 + Cntnap5b NNNATGTCAGGTCATTCACA NTG 2 0.0308 Tier II
20 NC_000085.6 19 47781828 + Cfap43 NNNATGTCACGTTATTGACA NGG 2 0.0235 Tier II
21 NC_000073.6 7 97217192 + Gab2 NNNATGTCAGGTTATTTACA NTG 2 0.0182 Tier II
22 NC_000067.6 1 174223166 - Spta1 NNNATGTCAGGTTATTCATA NGC 2 0.0103 Tier II
23 NC_000081.6 15 79625125 + Fam227a NNNATGTCAGGTGATTCACA NTG 2 0.0102 Tier II
24 NC_000085.6 19 43377906 - Hpse2 NNNCTGTCAGGTTATTCACA NGT 2 0.0057 Tier II
25 NC_000069.6 3 76645808 + Fstl5 NNNATGTCAGGTTATTCACA NTT 2 0.0 Tier II
26 NC_000068.7 2 132216977 - Gm14051 NNNTTGTCAGGTTATTCACT NGG 2 0.3818 Tier III
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Other clones with same target sequence:

(none)