GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001146011

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: AAAAGAGAAAAGAGAAACGT

Original Target:

Taxon:: Homo sapiens (human)
Gene:: CDK11B (984)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 77065

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000001.11	1	1652470	-	CDK11B	NNNAGAGAAAAGAGAAACGT	NGG	0	1.0	Tier I
2	NC_000001.11	1	1719359	-	CDK11A	NNNAGAGAAAAGAAAAATGT	NGG	2	0.4821	Tier I
3	NC_000016.10	16	68538845	-	ZFP90	NNNAGAGAAAAAAGAAACTT	NGG	2	0.6222	Tier II
4	NC_000022.11	22	38984874	-	APOBEC3B	NNNAGAGAAAAAAGAAATGT	NGG	2	0.6	Tier II
5	NC_000019.10	19	46302903	+	HIF3A	NNNAAAGAAAAGAGAAACTT	NGG	2	0.5778	Tier II
6	NC_000004.12	4	173470092	-	SCRG1	NNNAGAAAAAAGAGAAACGA	NGG	2	0.5625	Tier II
7	NC_000002.12	2	27478815	+	IFT172	NNNAGAAAAAAGAGAAAAGT	NGG	2	0.5385	Tier II
8	NC_000016.10	16	77425277	+	ADAMTS18	NNNAAAGAAAAGAGAAACGA	NGG	2	0.4875	Tier II
9	NC_000008.11	8	113241079	-	CSMD3	NNNAAAGAAAAGAGAAAAGT	NGG	2	0.4667	Tier II
10	NC_000001.11	1	156267455	+	SMG5	NNNAGAGAAAAGAGGAACAT	NGG	2	0.4643	Tier II
11	NC_000023.11	X	105633074	+	IL1RAPL2	NNNAGAGAAAAGAGAAATAT	NGG	2	0.4592	Tier II
12	NC_000001.11	1	53051676	+	SCP2	NNNAGAATAAAGAGAAACGT	NGG	2	0.4286	Tier II
13	NC_000002.12	2	28274864	+	BABAM2	NNNTGAGAAAAGAGAAACTT	NGG	2	0.4242	Tier II
14	NC_000010.11	10	77539162	-	KCNMA1	NNNACAGAAAAGAGAAAAGT	NGG	2	0.4231	Tier II
15	NC_000017.11	17	39738496	-	GRB7	NNNACAGAAAAGAGAAAAGT	NGG	2	0.4231	Tier II
16	NC_000001.11	1	8631152	-	RERE	NNNGGAGAAAAGAGAAATGT	NGG	2	0.4018	Tier II
17	NC_000012.12	12	90987880	-	EPYC	NNNAAAGAAAAGAGAAACCT	NGG	2	0.3885	Tier II
18	NC_000006.12	6	35360314	+	PPARD	NNNAGTGAAAAGAGAAAAGT	NGG	2	0.3846	Tier II
19	NC_000009.12	9	92389792	-	OGN	NNNAGATAAAAGAGAAACTT	NGG	2	0.381	Tier II
20	NC_000009.12	9	92389792	-	CENPP	NNNAGATAAAAGAGAAACTT	NGG	2	0.381	Tier II
21	NC_000003.12	3	169598905	+	MECOM	NNNAGAGAAGAGAGAAACTT	NGG	2	0.3704	Tier II
22	NC_000023.11	X	78315039	+	CYSLTR1	NNNAGACAAAAGAGAAAAGT	NGG	2	0.3702	Tier II
23	NC_000004.12	4	89287797	+	GPRIN3	NNNAGAGAAGAGAGAAATGT	NGG	2	0.3571	Tier II
24	NC_000005.10	5	112158168	+	EPB41L4A	NNNAGAGAAAAGAGGAAAGT	NGG	2	0.35	Tier II
25	NC_000003.12	3	174009762	+	NLGN1	NNNGGAGAAAAGAGAAAAGT	NGG	2	0.3365	Tier II
26	NC_000006.12	6	53903508	-	LRRC1	NNNGGAGAAAAGAGAAAAGT	NGG	2	0.3365	Tier II
27	NC_000009.12	9	76459950	-	GCNT1	NNNAGAGGAAAGAGAAACCT	NGG	2	0.3287	Tier II
28	NC_000001.11	1	170695807	-	PRRX1	NNNAGAGAAAAGAGAAAAGA	NGG	2	0.3029	Tier II
29	NC_000005.10	5	138901159	-	CTNNA1	NNNAGAGAAAAGAGAAAAGA	NGG	2	0.3029	Tier II
30	NC_000005.10	5	177707357	+	FAM153A	NNNAGACCAAAGAGAAACGT	NGG	2	0.2946	Tier II
31	NC_000011.10	11	89495935	+	NOX4	NNNAGAGAAAAGAGGAACCT	NGG	2	0.2914	Tier II
32	NC_000014.9	14	33523352	+	NPAS3	NNNAGAGAAAGGAGAAACCT	NGG	2	0.2914	Tier II
33	NC_000001.11	1	111693613	+	RAP1A	NNNAAAGAAATGAGAAACGT	NGG	2	0.2667	Tier II
34	NC_000004.12	4	97999412	+	STPG2	NNNAGAGAAAAGAGAAACCA	NGG	2	0.2522	Tier II
35	NC_000014.9	14	51826837	+	GNG2	NNNAGAGAAAAGAGAAACCA	NGG	2	0.2522	Tier II
36	NC_000004.12	4	106099805	-	TBCK	NNNAGAGAAAAGACAAAAGT	NGG	2	0.2308	Tier II
37	NC_000004.12	4	100474522	+	EMCN	NNNAGAGAAATGAAAAACGT	NGG	2	0.2308	Tier II
38	NC_000011.10	11	7437176	-	SYT9	NNNAGAGAAATGAGAAACTT	NGG	2	0.2051	Tier II
39	NC_000002.12	2	24570882	+	NCOA1	NNNAGAAAAAAGAGAAACGG	NGG	2	0.1765	Tier II
40	NC_000013.11	13	98383957	-	FARP1	NNNAGAGAAAAGAGGAACGT	NAG	2	0.1685	Tier II
41	NC_000012.12	12	1328606	-	ERC1	NNNAGAGAAAGGAGAAACGT	NAG	2	0.1685	Tier II
42	NC_000019.10	19	13227801	+	CACNA1A	NNNAGAGAAAAGAGAAAAGT	NAG	2	0.1396	Tier II
43	NC_000017.11	17	17985036	-	DRC3	NNNAGAGAAATGAGAAACCT	NGG	2	0.1379	Tier II
44	NC_000019.10	19	8914422	+	MUC16	NNNAGAGAAATGAGAAACCT	NGG	2	0.1379	Tier II
45	NC_000019.10	19	51313565	-	IGLON5	NNNAGAAAAAAGAGAAAGGT	NGG	2	0.1333	Tier II
46	NC_000001.11	1	220127420	+	IARS2	NNNAGAGAAAGGAGAGACGT	NGG	2	0.125	Tier II
47	NC_000007.14	7	48343514	-	ABCA13	NNNAGAGAAAAGAGAACCTT	NGG	2	0.1176	Tier II
48	NC_000014.9	14	22574060	-	DAD1	NNNAGAGAAAAGAGAACCTT	NGG	2	0.1176	Tier II
49	NC_000014.9	14	63277853	+	RHOJ	NNNAGAGAAAAGAGAGAAGT	NGG	2	0.1036	Tier II
50	NC_000006.12	6	4039862	-	PRP4K	NNNAGAGAAAAGAGAAAGAT	NGG	2	0.0952	Tier II
51	NC_000003.12	3	97662153	+	EPHA6	NNNAGAGAAAAGAGAAAAGG	NGG	2	0.095	Tier II
52	NC_000011.10	11	3934009	-	STIM1	NNNAGAGAAAAGAGAATCTT	NGG	2	0.0889	Tier II
53	NC_000007.14	7	108088671	-	LAMB4	NNNAGAGAGAAGAGAAAGGT	NGG	2	0.0889	Tier II
54	NC_000001.11	1	117172193	+	VTCN1	NNNAGAGAAAAAAGAAACGC	NGG	2	0.0848	Tier II
55	NC_000006.12	6	47994224	+	PTCHD4	NNNTGAGAAAAGAGAAAGGT	NGG	2	0.0848	Tier II
56	NC_000017.11	17	15931198	+	ADORA2B	NNNAAAGAAAAGAGAAACGC	NGG	2	0.0788	Tier II
57	NC_000015.10	15	53861835	-	UNC13C	NNNAGAGACAAGAGAAAGGT	NGG	2	0.0762	Tier II
58	NC_000003.12	3	124286195	-	KALRN	NNNAGAGAAAAGAGAAAGGA	NGG	2	0.075	Tier II
59	NC_000011.10	11	43607240	+	HSD17B12	NNNAGAGAAAAGAGAAAGGA	NGG	2	0.075	Tier II
60	NC_000002.12	2	102640000	+	SLC9A2	NNNAGAGAAAACAGAAAGGT	NGG	2	0.0706	Tier II
61	NC_000006.12	6	35962800	-	SLC26A8	NNNAGAGAAAAGAGAAACGT	NGA	1	0.0694	Tier II
62	NC_000001.11	1	183099934	-	LAMC1	NNNAGAGAACAGAGAAGCGT	NGG	2	0.0588	Tier II
63	NC_000003.12	3	33318347	-	FBXL2	NNNAGAGAAAAGAGAAACTT	NGA	2	0.0463	Tier II
64	NC_000019.10	19	1163262	+	SBNO2	NNNAGAGAAAAGAGGAACGT	NGA	2	0.0451	Tier II
65	NC_000023.11	X	101369879	-	BTK	NNNAGAGAAAGGAGAAACGT	NGA	2	0.0451	Tier II
66	NC_000003.12	3	165082385	-	SI	NNNAGAGAAAAGAGAAAAGT	NGA	2	0.0374	Tier II
67	NC_000016.10	16	10104287	+	GRIN2A	NNNAGAGAAAAGAGAAAAGT	NGA	2	0.0374	Tier II
68	NC_000008.11	8	68044741	+	PREX2	NNNAGAGAAAAGAGAAAGGT	NAG	2	0.0346	Tier II
69	NC_000002.12	2	196429660	+	HECW2	NNNAGAGAAAAGAGAAACTT	NTG	2	0.026	Tier II
70	NC_000009.12	9	16545959	+	BNC2	NNNAGAGAAAAGAGAAACTT	NTG	2	0.026	Tier II
71	NC_000001.11	1	49250249	+	AGBL4	NNNAGAGAAAAGAGAAATGT	NTG	2	0.025	Tier II
72	NC_000022.11	22	48591163	-	TAFA5	NNNAGAGAAAAGAGAAATGT	NTG	2	0.025	Tier II
73	NC_000007.14	7	38856616	+	VPS41	NNNAGAGAAAAGAGAAAGGG	NGG	2	0.0235	Tier II
74	NC_000005.10	5	36120972	-	LMBRD2	NNNAGAGAAAAGAGAAAAGT	NTG	2	0.021	Tier II
75	NC_000002.12	2	143307657	+	ARHGAP15	NNNAGAGAAAAGAGAAACTT	NGC	2	0.0148	Tier II
76	NC_000014.9	14	23057074	+	CDH24	NNNAGAGAAAAGAGAAAGGC	NGG	2	0.0121	Tier II
77	NC_000023.11	X	125109648	+	TENM1	NNNAGAGAAAAGAGAAACAT	NGT	2	0.0115	Tier II
78	NC_000007.14	7	140519808	+	DENND2A	NNNAGAGAAAAGAGAAACCT	NGT	2	0.0072	Tier II
79	NC_000012.12	12	10634881	-	STYK1	NNNAGAGAAAAGAGAAACGC	NGA	2	0.0063	Tier II
80	NC_000011.10	11	7575435	-	PPFIBP2	NNNAGAGAAAAGAGAAAGGT	NGC	2	0.003	Tier II
81	NC_000008.11	8	103956025	+	RIMS2	NNNAGAGAAAAAAGATACGT	NGG	2	0.0	Tier II
82	NC_000012.12	12	6584383	+	CHD4	NNNAGAGAAAAGAGATACGT	NTG	2	0.0	Tier II
83	NC_000021.9	21	42561387	-	SLC37A1	NNNAGTGAAAAGAGACACGT	NGG	2	0.0	Tier II
84	NC_000004.12	4	173470092	-	LOC112267917	NNNAGAAAAAAGAGAAACGA	NGG	2	0.5625	Tier III
85	NC_000002.12	2	107551308	-	LINC01886	NNNAGAGAAAAGGAAAACGT	NGG	2	0.4891	Tier III
86	NC_000009.12	9	23617753	+	LOC101929563	NNNAGAGAAAAGAAAAATGT	NGG	2	0.4821	Tier III
87	NC_000008.11	8	113241079	-	LOC107986966	NNNAAAGAAAAGAGAAAAGT	NGG	2	0.4667	Tier III
88	NC_000007.14	7	153392754	+	LOC105375577	NNNGGAGAAAAGAGAAACAT	NGG	2	0.4464	Tier III
89	NC_000002.12	2	28274864	+	LOC124907745	NNNTGAGAAAAGAGAAACTT	NGG	2	0.4242	Tier III
90	NC_000002.12	2	180122811	-	EPCART	NNNAGAGAAAAAAGAAACCT	NGG	2	0.4184	Tier III
91	NC_000018.10	18	154074	+	LOC105371951	NNNAGAGAAGAGAGAAACAT	NGG	2	0.3968	Tier III
92	NC_000018.10	18	31361071	-	DSG1-AS1	NNNAGGGAAAAGAGAAAAGT	NGG	2	0.3846	Tier III
93	NC_000018.10	18	44475960	-	LINC01478	NNNGGAGAAAAGAGAAAAGT	NGG	2	0.3365	Tier III
94	NC_000005.10	5	65090329	+	LOC107986419	NNNAGAGAAAAGTGAAACGT	NGG	1	0.3	Tier III
95	NC_000005.10	5	177707357	+	LOC107986489	NNNAGACCAAAGAGAAACGT	NGG	2	0.2946	Tier III
96	NC_000014.9	14	33523352	+	LOC124903299	NNNAGAGAAAGGAGAAACCT	NGG	2	0.2914	Tier III
97	NC_000002.12	2	12453230	-	MIR3681HG	NNNAGAGAAAAGACAAATGT	NGG	2	0.2755	Tier III
98	NC_000002.12	2	12453230	-	LOC105373431	NNNAGAGAAAAGACAAATGT	NGG	2	0.2755	Tier III
99	NC_000004.12	4	100474522	+	LOC124900740	NNNAGAGAAATGAAAAACGT	NGG	2	0.2308	Tier III
100	NC_000006.12	6	96370101	-	UFL1-AS1	NNNATAGAAAAGAGAAACAT	NGG	2	0.2143	Tier III
101	NC_000021.9	21	15425826	-	LOC101927745	NNNAGAGAAATGAGAAATGT	NGG	2	0.1978	Tier III
102	NC_000021.9	21	15425826	-	LOC124905055	NNNAGAGAAATGAGAAATGT	NGG	2	0.1978	Tier III
103	NC_000002.12	2	133923721	+	LOC105373627	NNNACAGAAAAGAGAAGCGT	NGG	2	0.1387	Tier III
104	NC_000017.11	17	17985036	-	LOC107984989	NNNAGAGAAATGAGAAACCT	NGG	2	0.1379	Tier III
105	NC_000004.12	4	151912566	+	LOC127898557	NNNAGAGGAAAGAGAAACGG	NGG	2	0.1294	Tier III
106	NC_000001.11	1	19022330	+	LOC105376815	NNNAGAGAAAAGAGAGAAGT	NGG	2	0.1036	Tier III
107	NC_000007.14	7	108088671	-	LOC105375446	NNNAGAGAGAAGAGAAAGGT	NGG	2	0.0889	Tier III
108	NC_000008.11	8	64189519	+	LINC01414	NNNAGAGAAAAGAGAAAGGA	NGG	2	0.075	Tier III
109	NC_000009.12	9	129445510	-	LOC105376291	NNNAGAGAAAAGAGAAAGGA	NGG	2	0.075	Tier III
110	NC_000007.14	7	143509639	-	EPHA1-AS1	NNNAGAGAAAAGAGAAACTT	NGA	2	0.0463	Tier III
111	NC_000002.12	2	8135738	+	LINC00299	NNNGGAGAAAAGAGAAACGT	NGA	2	0.0434	Tier III
112	NC_000009.12	9	7345541	-	LOC102723994	NNNAGAGAAAAGAGAAAGGT	NAG	2	0.0346	Tier III
113	NC_000009.12	9	7345541	-	LOC105375970	NNNAGAGAAAAGAGAAAGGT	NAG	2	0.0346	Tier III
114	NC_000002.12	2	196429660	+	LOC105373822	NNNAGAGAAAAGAGAAACTT	NTG	2	0.026	Tier III
115	NC_000022.11	22	48591163	-	LOC124900480	NNNAGAGAAAAGAGAAATGT	NTG	2	0.025	Tier III
116	NC_000014.9	14	44177308	+	LINC02307	NNNAGAGAAAAGAGAAACGT	NGT	1	0.0161	Tier III
117	NC_000005.10	5	23984139	-	LINC02899	NNNAGAGAAAAGAGAAAGGC	NGG	2	0.0121	Tier III
118	NC_000011.10	11	7575435	-	LOC105376535	NNNAGAGAAAAGAGAAAGGT	NGC	2	0.003	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000070.6	4	155628793	+	Cdk11b	NNNAAAGAAAAGAGAAACGT	NGA	2	0.0602	Tier I
2	NC_000073.6	7	52003970	-	Svip	NNNAGAGAAAAGAGAAAAGC	NGG	2	0.049	Tier I
3	NC_000071.6	5	123611875	-	Clip1	NNNAGAGAAAACAGAAACGC	NGG	2	0.0481	Tier I
4	NC_000069.6	3	10421330	+	Snx16	NNNAGAAAAAAGAGAAACTT	NGG	2	0.6667	Tier II
5	NC_000080.6	14	93867163	+	Pcdh9	NNNAGAGAAAAAAGAAATGT	NGG	2	0.6	Tier II
6	NC_000071.6	5	113982557	-	Ssh1	NNNAGAAACAAGAGAAACGT	NGG	2	0.5714	Tier II
7	NC_000079.6	13	91030423	-	Atg10	NNNAGAGAAAAGAAAAACAT	NGG	2	0.5357	Tier II
8	NC_000077.6	11	6405882	-	Zmiz2	NNNACAGAAAAGAGAAACTT	NGG	2	0.5238	Tier II
9	NC_000080.6	14	36877931	+	Ccser2	NNNACAGAAAAGAGAAACTT	NGG	2	0.5238	Tier II
10	NC_000082.6	16	30974857	-	Xxylt1	NNNAAAGAAGAGAGAAACGT	NGG	2	0.4815	Tier II
11	NC_000073.6	7	76916352	-	Agbl1	NNNTGAGAAAAGAAAAACGT	NGG	2	0.4773	Tier II
12	NC_000072.6	6	46717867	+	Cntnap2	NNNAAAGAAAAGAGAAAAGT	NGG	2	0.4667	Tier II
13	NC_000086.7	X	86810494	+	Il1rapl1	NNNAAAGAAAAGAGAAAAGT	NGG	2	0.4667	Tier II
14	NC_000075.6	9	69317334	-	Rora	NNNAGGGAAAAGAGAAATGT	NGG	2	0.4592	Tier II
15	NC_000084.6	18	36075384	-	Nrg2	NNNAGAGAGAAGAGAAATGT	NGG	2	0.4286	Tier II
16	NC_000074.6	8	119130508	+	Cdh13	NNNAGACAAGAGAGAAACGT	NGG	2	0.3819	Tier II
17	NC_000084.6	18	37962409	-	Fchsd1	NNNAGAGAAAAGAGAAACTA	NGG	2	0.375	Tier II
18	NC_000072.6	6	35028514	-	Cnot4	NNNAGAGAAGAGGGAAACGT	NGG	2	0.3623	Tier II
19	NC_000072.6	6	27984718	-	Grm8	NNNAGAGAAAAGGGAAAAGT	NGG	2	0.3512	Tier II
20	NC_000072.6	6	77117530	+	Ctnna2	NNNAGAGAAAAGGGAAAAGT	NGG	2	0.3512	Tier II
21	NC_000078.6	12	83650471	-	Rbm25	NNNAGAGAAAAGGGAAAAGT	NGG	2	0.3512	Tier II
22	NC_000083.6	17	65429843	+	Tmem232	NNNGGAGAAAAGAGAAAAGT	NGG	2	0.3365	Tier II
23	NC_000072.6	6	111249785	-	Grm7	NNNAGAGAAAAGAGAAAAGA	NGG	2	0.3029	Tier II
24	NC_000075.6	9	21651312	-	Smarca4	NNNAGAGAAAAGAGAAAAGA	NGG	2	0.3029	Tier II
25	NC_000075.6	9	40933670	-	Jhy	NNNAGAGAAAAGAGAAAAGA	NGG	2	0.3029	Tier II
26	NC_000084.6	18	65723423	+	Oacyl	NNNAGAGAAAAGAGAAAAGA	NGG	2	0.3029	Tier II
27	NC_000067.6	1	70885430	-	Vwc2l	NNNAGAGAAAGGAGAAACCT	NGG	2	0.2914	Tier II
28	NC_000073.6	7	130716682	-	Tacc2	NNNAGAGAAATAAGAAACGT	NGG	2	0.2872	Tier II
29	NC_000078.6	12	4158500	+	Adcy3	NNNGGAGAAAAGAGAAACCT	NGG	2	0.2802	Tier II
30	NC_000083.6	17	67805502	-	Lama1	NNNAGAGAAAGTAGAAACGT	NGG	2	0.25	Tier II
31	NC_000078.6	12	100031638	-	Kcnk13	NNNAGAGAAAACAGAAACCT	NGG	2	0.2373	Tier II
32	NC_000072.6	6	5774808	-	Dync1i1	NNNAGAGTAAAGAGAAAAGT	NGG	2	0.2308	Tier II
33	NC_000075.6	9	32127901	-	Arhgap32	NNNAGAGAAATGAGAAACAT	NGG	2	0.2198	Tier II
34	NC_000072.6	6	72327368	+	Usp39	NNNAGAGAAATGAGAAATGT	NGG	2	0.1978	Tier II
35	NC_000072.6	6	116654635	-	Rassf4	NNNAGAGAAATGAGAAATGT	NGG	2	0.1978	Tier II
36	NC_000071.6	5	125775700	-	Tmem132b	NNNAGAGAAAAGAAAAACGT	NAG	2	0.1944	Tier II
37	NC_000072.6	6	92896556	+	Adamts9	NNNAGAGAACAGAGAAAAGT	NGG	2	0.1795	Tier II
38	NC_000081.6	15	88895834	+	Ttll8	NNNAGATAAAAGAGAAACGT	NAG	2	0.1481	Tier II
39	NC_000070.6	4	144961097	-	Vps13d	NNNAGAGAAAAGAGCAAAGT	NGG	2	0.1469	Tier II
40	NC_000079.6	13	94115014	-	Lhfpl2	NNNAGAGAAAAGAAAGACGT	NGG	2	0.1442	Tier II
41	NC_000078.6	12	20451129	+	Gm29758	NNNAGAGGAAAGAGAAACGG	NGG	2	0.1294	Tier II
42	NC_000078.6	12	20451129	+	Gm10479	NNNAGAGGAAAGAGAAACGG	NGG	2	0.1294	Tier II
43	NC_000073.6	7	76359922	+	Agbl1	NNNAGAGAAAAAAGAAAGGT	NGG	2	0.1244	Tier II
44	NC_000072.6	6	149419278	+	Bicd1	NNNAGAGAAAAGAGAGATGT	NGG	2	0.1236	Tier II
45	NC_000079.6	13	34991397	-	Eci2	NNNAGAGAAAAGAGCAACCT	NGG	2	0.1223	Tier II
46	NC_000067.6	1	97460953	+	Gm7135	NNNAGAGAAAAGAGAAACTG	NGG	2	0.1176	Tier II
47	NC_000069.6	3	65197887	+	Kcnab1	NNNAGAGAAAAGAGAAACTG	NGG	2	0.1176	Tier II
48	NC_000080.6	14	37105841	-	2610528A11Rik	NNNAGAGAAAAGAGAAACTG	NGG	2	0.1176	Tier II
49	NC_000071.6	5	116057854	-	Tmem233	NNNAGAGAAAAGGGAAACGG	NGG	2	0.1151	Tier II
50	NC_000077.6	11	20332631	+	Slc1a4	NNNAGATAAAAGAGAGACGT	NGG	2	0.1099	Tier II
51	NC_000071.6	5	115986491	-	Cit	NNNAGAGAAAAGAGAGACGA	NGG	2	0.1082	Tier II
52	NC_000076.6	10	23114874	+	Eya4	NNNAGAGAAAAGAGAAAAGG	NGG	2	0.095	Tier II
53	NC_000084.6	18	10731336	-	Mib1	NNNAGAGAAAAGAGAAAAGG	NGG	2	0.095	Tier II
54	NC_000073.6	7	100416790	-	C2cd3	NNNAGAGAGAAGAGAAAGGT	NGG	2	0.0889	Tier II
55	NC_000075.6	9	97459096	+	Clstn2	NNNAGAGAAACGAGAGACGT	NGG	2	0.0769	Tier II
56	NC_000068.7	2	157220635	-	Mroh8	NNNAGAGAAAAGAGAAAGGA	NGG	2	0.075	Tier II
57	NC_000071.6	5	63752360	-	Nwd2	NNNAGAGAAGAGAGAAAGGT	NGG	2	0.0741	Tier II
58	NC_000075.6	9	23343235	-	Bmper	NNNAGAGAAAAGAGAAACAC	NGG	2	0.0649	Tier II
59	NC_000068.7	2	165492859	+	Trp53rka	NNNAGAGAAATGAGAAACGG	NGG	2	0.0543	Tier II
60	NC_000070.6	4	104416171	+	Dab1	NNNAGAGGAAAGAGAAACGT	NGA	2	0.0509	Tier II
61	NC_000077.6	11	69196737	+	Alox8	NNNAGAGAAAAGAGAAACGG	NAG	2	0.0458	Tier II
62	NC_000081.6	15	36478115	+	Ankrd46	NNNAGAGAAAAGAGAACCGT	NAG	2	0.0458	Tier II
63	NC_000080.6	14	77582963	+	Enox1	NNNAGAGAAAAGAGAAATGT	NGA	2	0.0446	Tier II
64	NC_000084.6	18	75593525	+	Ctif	NNNAGAGAAAAGAGAAACCC	NGG	2	0.0408	Tier II
65	NC_000069.6	3	33141584	-	Pex5l	NNNAGAGAAAAGAGAAACAT	NTG	2	0.0278	Tier II
66	NC_000077.6	11	107303201	-	Pitpnc1	NNNAGAGAAAAGAGAAATGT	NTG	2	0.025	Tier II
67	NC_000080.6	14	123303115	-	Nalcn	NNNAGAGAAAAGAGAAAGGG	NGG	2	0.0235	Tier II
68	NC_000081.6	15	5197421	-	Ttc33	NNNAGAGAAAAGAGAAGGGT	NGG	2	0.0235	Tier II
69	NC_000072.6	6	56170703	+	Pde1c	NNNAGAGAAAAGAGAAAAGT	NTG	2	0.021	Tier II
70	NC_000072.6	6	119801184	+	Erc1	NNNAGAGAAAAGAGAAACCT	NGT	2	0.0072	Tier II
71	NC_000072.6	6	80210848	-	Lrrtm4	NNNAGAGAAAAGAGAAACGT	NTC	2	0.0	Tier II
72	NC_000075.6	9	53812328	-	Slc35f2	NNNAGAGAAAAGAGAAACGT	NAT	2	0.0	Tier II
73	NC_000083.6	17	73893734	+	Xdh	NNNAGAGAAAAGAGAAACGT	NTA	2	0.0	Tier II
74	NC_000080.6	14	122767194	+	Pcca	NNNAGAGAAAAGAGACACGT	NAG	2	0.0	Tier II
75	NC_000077.6	11	5333927	-	Znrf3	NNNAGAGAAAAGGGACACGT	NGG	2	0.0	Tier II
76	NC_000069.6	3	128723036	+	Gm40148	NNNAGAGAAAAGAGAAACAT	NGG	1	0.7143	Tier III
77	NC_000080.6	14	106265303	+	Gm36107	NNNAGAGAAAAGAGAAAAGT	NGG	1	0.5385	Tier III
78	NC_000069.6	3	54519446	-	Gm31154	NNNAGAGAAAAGAAAAACAT	NGG	2	0.5357	Tier III
79	NC_000082.6	16	30974857	-	Gm15742	NNNAAAGAAGAGAGAAACGT	NGG	2	0.4815	Tier III
80	NC_000069.6	3	17579846	-	Gm30340	NNNAGAGAGAAGAGAAACTT	NGG	2	0.4444	Tier III
81	NC_000084.6	18	36075384	-	Gm41694	NNNAGAGAGAAGAGAAATGT	NGG	2	0.4286	Tier III
82	NC_000074.6	8	80392782	-	Gm30605	NNNAGAGAAAAGAGAAAAGA	NGG	2	0.3029	Tier III
83	NC_000076.6	10	91258963	-	Gm18705	NNNAGAGAAAAGAGAAAAGA	NGG	2	0.3029	Tier III
84	NC_000067.6	1	70885430	-	Gm29906	NNNAGAGAAAGGAGAAACCT	NGG	2	0.2914	Tier III
85	NC_000073.6	7	130716682	-	Gm34283	NNNAGAGAAATAAGAAACGT	NGG	2	0.2872	Tier III
86	NC_000075.6	9	95443472	+	Gm32133	NNNAGAGTTAAGAGAAACGT	NGG	2	0.2571	Tier III
87	NC_000084.6	18	8680213	-	Gm35180	NNNAGAGAAATGAGAAATGT	NGG	2	0.1978	Tier III
88	NC_000081.6	15	88895834	+	Gm38643	NNNAGATAAAAGAGAAACGT	NAG	2	0.1481	Tier III
89	NC_000070.6	4	17558557	+	Gm30092	NNNAGAGAAAAGAGAAAAGT	NAG	2	0.1396	Tier III
90	NC_000078.6	12	21744362	-	6030426L16Rik	NNNAGAGGAAAGAGAAACGG	NGG	2	0.1294	Tier III
91	NC_000078.6	12	21744362	-	Gm4036	NNNAGAGGAAAGAGAAACGG	NGG	2	0.1294	Tier III
92	NC_000082.6	16	76022649	-	Gm15555	NNNAGAGAAAAGAGAAACAG	NGG	2	0.1261	Tier III
93	NC_000073.6	7	76359922	+	Gm30774	NNNAGAGAAAAAAGAAAGGT	NGG	2	0.1244	Tier III
94	NC_000069.6	3	65197887	+	A330015K06Rik	NNNAGAGAAAAGAGAAACTG	NGG	2	0.1176	Tier III
95	NC_000076.6	10	97843688	-	Gm10754	NNNAGAGAAAAGAGAATCAT	NGG	2	0.0952	Tier III
96	NC_000076.6	10	25644691	-	Gm29571	NNNAGAGAAAAGAGAAACGC	NGG	1	0.0909	Tier III
97	NC_000081.6	15	18059736	-	Gm41277	NNNAGAGAAAAGAGAAACCG	NGG	2	0.0791	Tier III
98	NC_000078.6	12	113486357	-	Igh	NNNAGAGAAAAGAGAAACGA	NGA	2	0.0391	Tier III
99	NC_000077.6	11	90215145	+	Gm42089	NNNAGAGAAAAGAGAAAGGG	NGG	2	0.0235	Tier III
100	NC_000079.6	13	48101167	+	A330033J07Rik	NNNAGAGAAAAGAGAAAGGG	NGG	2	0.0235	Tier III
101	NC_000082.6	16	26267621	+	Gm41436	NNNAGAGAAAAGAGAAAAGT	NGT	2	0.0087	Tier III
102	NC_000073.6	7	132907205	-	1500002F19Rik	NNNAGAGAAAAGAGACAGGT	NGG	2	0.0	Tier III

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Other clones with same target sequence:

(none)