Construct: sgRNA BRDN0001146013

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGATGCAGATCAAATGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCYL2 (55681)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76052

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 100313534 + SCYL2 NNNTGCAGATCAAATGACAA NGG 0 1.0 Tier I
2 NC_000005.10 5 66843374 - MAST4 NNNTGTAGATCAAATGACAG NGG 2 0.7101 Tier II
3 NC_000006.12 6 134254664 - SGK1 NNNTGTATATCAAATGACAA NGG 2 0.5804 Tier II
4 NC_000007.14 7 96665689 + SEM1 NNNTGTACATCAAATGACAA NGG 2 0.5714 Tier II
5 NC_000009.12 9 127305004 + GARNL3 NNNTGCAGACCGAATGACAA NGG 2 0.3852 Tier II
6 NC_000015.10 15 57086079 - TCF12 NNNTGCTGAACAAATGACAA NGG 2 0.375 Tier II
7 NC_000014.9 14 79062649 + NRXN3 NNNTGCAGATCAGTTGACAA NGG 2 0.3478 Tier II
8 NC_000015.10 15 86544969 - AGBL1 NNNTGCAAATCATATGACAA NGG 2 0.3 Tier II
9 NC_000018.10 18 26384775 - TAF4B NNNTGAAGATTAAATGACAA NGG 2 0.2857 Tier II
10 NC_000001.11 1 95241811 + RWDD3 NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier II
11 NC_000001.11 1 95241811 + TLCD4-RWDD3 NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier II
12 NC_000003.12 3 126066928 - SLC41A3 NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier II
13 NC_000016.10 16 19662529 + VPS35L NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier II
14 NC_000008.11 8 116761784 - EIF3H NNNTTCAGGTCAAATGACAA NGG 2 0.2 Tier II
15 NC_000003.12 3 27113943 + NEK10 NNNTGCAGATAAAATGACAA NAG 2 0.1944 Tier II
16 NC_000001.11 1 65177178 + AK4 NNNTGCAGACTAAATGACAA NGG 2 0.1641 Tier II
17 NC_000011.10 11 47630918 + MTCH2 NNNTGCAGATCAAATGACAT NAG 2 0.1556 Tier II
18 NC_000005.10 5 107920467 - FBXL17 NNNTGCTGATTAAATGACAA NGG 2 0.1346 Tier II
19 NC_000006.12 6 131613947 + MED23 NNNTGCAGCTCAAATGACCA NGG 2 0.1182 Tier II
20 NC_000004.12 4 186589636 - FAT1 NNNTGCAGATCAAGTGACAC NGG 2 0.1061 Tier II
21 NC_000006.12 6 64304089 - EYS NNNTGCAGATTAAATGACAA NAG 2 0.0798 Tier II
22 NC_000002.12 2 201412349 - TRAK2 NNNTGCAGATCAAATGACAA NGA 1 0.0694 Tier II
23 NC_000012.12 12 16576467 + MGST1 NNNTTCAGATCAAATGTCAA NGG 2 0.04 Tier II
24 NC_000012.12 12 16576467 + LMO3 NNNTTCAGATCAAATGTCAA NGG 2 0.04 Tier II
25 NC_000016.10 16 53811501 - FTO NNNTGCAGATCAAATGGGAA NGG 2 0.0235 Tier II
26 NC_000016.10 16 69286256 - SNTB2 NNNTGCAGATTAAATGACAA NTG 2 0.012 Tier II
27 NC_000012.12 12 54397559 - ITGA5 NNNTGCAGATCCAATGACAA NTG 2 0.0103 Tier II
28 NC_000009.12 9 270096 - DOCK8 NNNTGCAGATCAAATCACAA NGA 2 0.0 Tier II
29 NC_000008.11 8 125499261 - TRIB1AL NNNTAGAGATCAAATGACAA NGG 2 0.4333 Tier III
30 NC_000023.11 X 71357272 - LOC124905198 NNNTCCAGATCAAATGAAAA NGG 2 0.4231 Tier III
31 NC_000023.11 X 103721836 - GLRA4 NNNTGCAGAGCAAATGACAG NGG 2 0.3824 Tier III
32 NC_000011.10 11 131217351 + LOC107984412 NNNTGCAGATCAAATGAATA NGG 2 0.2899 Tier III
33 NC_000002.12 2 177601332 - LOC124906098 NNNTGCAGACCAAATGACTA NGG 2 0.2872 Tier III
34 NC_000001.11 1 94813155 - SLC44A3-AS1 NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier III
35 NC_000002.12 2 113865295 - ACTR3-AS1 NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier III
36 NC_000004.12 4 41921695 - LOC105374426 NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier III
37 NC_000013.11 13 71026440 + LINC00348 NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier III
38 NC_000013.11 13 71030994 + LINC00348 NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier III
39 NC_000014.9 14 81781959 + LOC107984704 NNNTACAGATTAAATGACAA NGG 2 0.2667 Tier III
40 NC_000006.12 6 41381977 + LOC100505711 NNNTTCAGATCAGATGACAA NGG 2 0.1957 Tier III
41 NC_000012.12 12 106560693 - LOC100287944 NNNAGCAGATCCAATGACAA NGG 2 0.1253 Tier III
42 NC_000008.11 8 39409118 + ADAM5 NNNTGCAGATTTAATGACAA NGG 2 0.1026 Tier III
43 NC_000002.12 2 201412349 - SCYL2P1 NNNTGCAGATCAAATGACAA NGA 1 0.0694 Tier III
44 NC_000005.10 5 17425516 - LINC02217 NNNTGAAGATCAAATGACAA NGA 2 0.0645 Tier III
45 NC_000011.10 11 22165266 + LOC124902645 NNNTCCAGATCAAATGACAA NGA 2 0.0546 Tier III
46 NC_000003.12 3 61296860 - LOC105377114 NNNTGCAGATTAAATGGCAA NGG 2 0.0543 Tier III
47 NC_000010.11 10 2608529 + LOC105376350 NNNTGCAGATCCAATGCCAA NGG 2 0.0464 Tier III
48 NC_000012.12 12 118949077 + LOC105370020 NNNTGCAGATCCAAGGACAA NGG 2 0.0132 Tier III
49 NC_000012.12 12 54397559 - GPR84-AS1 NNNTGCAGATCCAATGACAA NTG 2 0.0103 Tier III
50 NC_000008.11 8 76488097 - LINC01111 NNNTGCAGATCAAATTACAA NAG 2 0.0 Tier III
51 NC_000008.11 8 76488097 - LOC107986952 NNNTGCAGATCAAATTACAA NAG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 89659244 - Scyl2 NNNTGCAGATCAAATGACAA NAG 1 0.2593 Tier I
2 NC_000077.6 11 21298771 + Vps54 NNNTGCAGATCAAATGAGAA NGT 2 0.0022 Tier I
3 NC_000067.6 1 144153839 - Rgs13 NNNTGCAAATCAAATGATAA NGG 2 0.6429 Tier II
4 NC_000086.7 X 51157988 + Mbnl3 NNNTGAAGATCAAATGACAT NGG 2 0.5571 Tier II
5 NC_000074.6 8 13120113 + Cul4a NNNTGAAGATCAAATGAAAA NGG 2 0.5 Tier II
6 NC_000070.6 4 142057509 - Tmem51 NNNTGCAGGTCAAATGATAA NGG 2 0.4286 Tier II
7 NC_000067.6 1 21209567 + Tmem14a NNNTGGAGAACAAATGACAA NGG 2 0.4286 Tier II
8 NC_000073.6 7 124235638 + Hs3st4 NNNTGGAGATAAAATGACAA NGG 2 0.375 Tier II
9 NC_000081.6 15 32433936 + Sema5a NNNTGCAGAGCATATGACAA NGG 2 0.15 Tier II
10 NC_000071.6 5 115717478 + Bicdl1 NNNTGCAGAACAAATGAGAA NGG 2 0.1143 Tier II
11 NC_000068.7 2 43918780 - Arhgap15 NNNTGCAGACCAAATGGCAA NGG 2 0.0941 Tier II
12 NC_000075.6 9 48710857 + Zbtb16 NNNTCCAGATCAAATGACAA NGA 2 0.0546 Tier II
13 NC_000074.6 8 91339013 - Fto NNNTGCAGATGAAATGGCAA NGG 2 0.0441 Tier II
14 NC_000075.6 9 123376969 - Lars2 NNNTGCACATCAAAGGACAA NGG 2 0.0308 Tier II
15 NC_000071.6 5 118008962 + Fbxo21 NNNTGCAGATCAAAGGAAAA NGG 2 0.0269 Tier II
16 NC_000077.6 11 109959217 - Abca8b NNNTGCAGATCACATGACAA NTG 2 0.0082 Tier II
17 NC_000083.6 17 85289101 + Camkmt NNNTTCAGATCAAATGACAA NGC 2 0.0067 Tier II
18 NC_000070.6 4 87877924 + Mllt3 NNNTGCAAATCAAATCACAA NGG 2 0.0 Tier II
19 NC_000075.6 9 59690745 - Gramd2 NNNTGCAGAGCAAATTACAA NGG 2 0.0 Tier II
20 NC_000081.6 15 55469168 - Col14a1 NNNTTCAGATCAAATCACAA NGG 2 0.0 Tier II
21 NC_000067.6 1 21209567 + Gm28836 NNNTGGAGAACAAATGACAA NGG 2 0.4286 Tier III
22 NC_000076.6 10 66171032 - 1700023F02Rik NNNTGCAGTTCAAAGGACAA NGG 2 0.03 Tier III
23 NC_000076.6 10 66171032 - 4930407I19Rik NNNTGCAGTTCAAAGGACAA NGG 2 0.03 Tier III
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Other clones with same target sequence:

(none)