Construct: sgRNA BRDN0001146016
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTCCCCTTGATCTCTACTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IPPK (64768)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77152
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
92642757 |
- |
IPPK |
NNNCCCTTGATCTCTACTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
9700168 |
+ |
USP43 |
NNNCCCTTGTTCTCTTCTCA |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000005.10 |
5 |
135356381 |
+ |
MACROH2A1 |
NNNCCCTTGTTCTTTACTCA |
NGG |
2 |
0.6471 |
Tier II |
| 4 |
NC_000010.11 |
10 |
114252338 |
- |
VWA2 |
NNNCCCTCAATCTCTACTCA |
NGG |
2 |
0.6429 |
Tier II |
| 5 |
NC_000021.9 |
21 |
31422768 |
+ |
TIAM1 |
NNNCTCATGATCTCTACTCA |
NGG |
2 |
0.5568 |
Tier II |
| 6 |
NC_000008.11 |
8 |
4953902 |
- |
CSMD1 |
NNNCCCTTGATCCCTACTCC |
NGG |
2 |
0.1794 |
Tier II |
| 7 |
NC_000002.12 |
2 |
69152327 |
- |
ANTXR1 |
NNNCCCTTTCTCTCTACTCA |
NGG |
2 |
0.1778 |
Tier II |
| 8 |
NC_000007.14 |
7 |
151039834 |
+ |
ABCB8 |
NNNCCCTTGATCTCTGTTCA |
NGG |
2 |
0.0897 |
Tier II |
| 9 |
NC_000023.11 |
X |
33050578 |
- |
DMD |
NNNCCCTTGATCTCTGCTCA |
NAG |
2 |
0.0499 |
Tier II |
| 10 |
NC_000005.10 |
5 |
135817345 |
- |
SLC25A48 |
NNNCCATTGATCTCTACTCA |
NTG |
2 |
0.0362 |
Tier II |
| 11 |
NC_000017.11 |
17 |
13576735 |
- |
HS3ST3A1 |
NNNCCCTTGATCTCTACTGC |
NGG |
2 |
0.0284 |
Tier II |
| 12 |
NC_000007.14 |
7 |
150268428 |
+ |
ACTR3C |
NNNCCCTTGATCTCTACTCA |
NTC |
2 |
0.0 |
Tier II |
| 13 |
NC_000007.14 |
7 |
152841455 |
- |
ACTR3B |
NNNCCCTTGATCTCTACTCA |
NTC |
2 |
0.0 |
Tier II |
| 14 |
NC_000020.11 |
20 |
47224082 |
- |
ZMYND8 |
NNNCCCTTGATCTCTTCTCA |
NGC |
2 |
0.0 |
Tier II |
| 15 |
NC_000007.14 |
7 |
57867472 |
- |
LOC102723842 |
NNNCCCGTGATCTCTACTCA |
NTG |
2 |
0.0229 |
Tier III |
| 16 |
NC_000002.12 |
2 |
23062856 |
+ |
LOC107985792 |
NNNCCCTTGATTTCTACTCA |
NTG |
2 |
0.021 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
49442007 |
+ |
Ippk |
NNNCCCTCGACCTCTACTCA |
NGG |
2 |
0.6667 |
Tier I |
| 2 |
NC_000077.6 |
11 |
43209847 |
+ |
Atp10b |
NNNCCCCTGACCTCTACTCA |
NGG |
2 |
0.4583 |
Tier II |
| 3 |
NC_000074.6 |
8 |
61399763 |
- |
Sh3rf1 |
NNNCACTAGATCTCTACTCA |
NGG |
2 |
0.4571 |
Tier II |
| 4 |
NC_000076.6 |
10 |
127515309 |
- |
Ndufa4l2 |
NNNCCCTTTATCTTTACTCA |
NGG |
2 |
0.3911 |
Tier II |
| 5 |
NC_000067.6 |
1 |
74476988 |
+ |
Usp37 |
NNNTCCTTGCTCTCTACTCA |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000075.6 |
9 |
4572045 |
- |
Gria4 |
NNNCCCTTGCTCTCTACTCA |
NAG |
2 |
0.0864 |
Tier II |
| 7 |
NC_000080.6 |
14 |
10439111 |
+ |
Fhit |
NNNCCCTTGTTCTCTACTCA |
NGC |
2 |
0.0196 |
Tier II |
| 8 |
NC_000074.6 |
8 |
73220732 |
- |
Large1 |
NNNGCCTTGATCTCTACTCA |
NTG |
2 |
0.0195 |
Tier II |
| 9 |
NC_000068.7 |
2 |
103442008 |
- |
Elf5 |
NNNCCCTTGATCTCTCCTTA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000068.7 |
2 |
160276753 |
+ |
Gm46773 |
NNNTCCTTGATCCCTACTCA |
NGG |
2 |
0.6316 |
Tier III |
| 11 |
NC_000073.6 |
7 |
84706405 |
- |
2610206C17Rik |
NNNCCCTTAAACTCTACTCA |
NGG |
2 |
0.4821 |
Tier III |
| 12 |
NC_000077.6 |
11 |
43209847 |
+ |
Gm12146 |
NNNCCCCTGACCTCTACTCA |
NGG |
2 |
0.4583 |
Tier III |
| 13 |
NC_000068.7 |
2 |
144830382 |
+ |
Gm39952 |
NNNCCCTTAATCTCTACTGA |
NGG |
2 |
0.0804 |
Tier III |
| 14 |
NC_000075.6 |
9 |
68557493 |
- |
Gm31141 |
NNNCCCTTGATCTCTACCCA |
NGC |
2 |
0.0095 |
Tier III |
| 15 |
NC_000076.6 |
10 |
96569841 |
+ |
Gm33843 |
NNNCCCTTGATGTCTCCTCA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
