Construct: sgRNA BRDN0001146020
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAAGGACACCAGATCGCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K19 (80122)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76693
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
134988221 |
+ |
MAP3K19 |
NNNAGGACACCAGATCGCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
240760111 |
+ |
KIF1A |
NNNAGGACACCAGCTCACGT |
NGG |
2 |
0.2 |
Tier II |
| 3 |
NC_000016.10 |
16 |
1687837 |
- |
JPT2 |
NNNAGGACTCCAGATCTCGT |
NGG |
2 |
0.15 |
Tier II |
| 4 |
NC_000007.14 |
7 |
155169762 |
+ |
LOC105375584 |
NNNAGGACACCAGATCACAT |
NGG |
2 |
0.6667 |
Tier III |
| 5 |
NC_000001.11 |
1 |
175045380 |
- |
ENTR1P2 |
NNNAGGACACCAGATGGAGT |
NGG |
2 |
0.0828 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
9370810 |
- |
Samd5 |
NNNAGGACACCAGATCCCCT |
NGG |
2 |
0.1055 |
Tier II |
| 2 |
NC_000075.6 |
9 |
108133684 |
- |
Bsn |
NNNAGGACACCAGATGGCCT |
NGG |
2 |
0.069 |
Tier II |
| 3 |
NC_000074.6 |
8 |
86645701 |
- |
Lonp2 |
NNNAGGACACCAGATCGCCT |
NGA |
2 |
0.0311 |
Tier II |
| 4 |
NC_000080.6 |
14 |
70314191 |
+ |
Slc39a14 |
NNNAGGACACCAGAGCGAGT |
NGG |
2 |
0.0269 |
Tier II |
| 5 |
NC_000067.6 |
1 |
75467799 |
- |
Asic4 |
NNNAGGACACCAGATGGCGC |
NGG |
2 |
0.014 |
Tier II |
Other clones with same target sequence:
(none)
