Construct: sgRNA BRDN0001146021
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGGGGACACTTACATGGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SIK1 (150094)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77894
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
6119238 |
- |
LOC102724428 |
NNNGGGACACTTACATGGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000021.9 |
21 |
43419024 |
+ |
SIK1 |
NNNGGGACACTTACATGGCG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000012.12 |
12 |
121750437 |
- |
TMEM120B |
NNNGGGACACTTACTTGGCC |
NGG |
2 |
0.0857 |
Tier I |
| 4 |
NC_000010.11 |
10 |
129664418 |
+ |
MGMT |
NNNGGGACACTTACATGGAG |
NAG |
2 |
0.1111 |
Tier II |
| 5 |
NC_000011.10 |
11 |
78794142 |
- |
TENM4 |
NNNGGGACACTTAGATGGCC |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000001.11 |
1 |
234674278 |
+ |
LOC124904555 |
NNNGGGACACTAACATGGGG |
NGG |
2 |
0.1 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
24895171 |
+ |
Mrps34 |
NNNAGGACACTGACATGGCG |
NGG |
2 |
0.45 |
Tier I |
| 2 |
NC_000076.6 |
10 |
121301468 |
+ |
Tbc1d30 |
NNNGGGACATTTACATGGCA |
NGG |
2 |
0.8824 |
Tier II |
| 3 |
NC_000076.6 |
10 |
95293504 |
- |
Cradd |
NNNGGTACACTAACATGGCG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000085.6 |
19 |
43835175 |
+ |
Abcc2 |
NNNGGGACACTTGCATGGCT |
NGG |
2 |
0.4565 |
Tier II |
| 5 |
NC_000079.6 |
13 |
69731725 |
- |
Ube2ql1 |
NNNGGGACACATACATGGCG |
NGA |
2 |
0.0521 |
Tier II |
| 6 |
NC_000084.6 |
18 |
76073302 |
- |
Zbtb7c |
NNNGGGACACTTACATGGCT |
NTG |
2 |
0.0273 |
Tier II |
| 7 |
NC_000069.6 |
3 |
94627130 |
- |
Tuft1 |
NNNGGGACACTTCCATGGCG |
NGT |
2 |
0.0034 |
Tier II |
| 8 |
NC_000078.6 |
12 |
28910590 |
- |
Gm31508 |
NNNGGGACACTTACAGGGAG |
NGG |
2 |
0.1484 |
Tier III |
Other clones with same target sequence:
(none)
