Construct: sgRNA BRDN0001146027
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGATACAGGTTTAATTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CD2 (914)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75826
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
116760484 |
- |
CD2 |
NNNGATACAGGTTTAATTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
26379484 |
+ |
SELENOI |
NNNGATACAGGTTTAATTTT |
NGG |
2 |
0.3231 |
Tier II |
| 3 |
NC_000002.12 |
2 |
69621724 |
- |
AAK1 |
NNNGATACAGGTTTAATGAG |
NGG |
2 |
0.1429 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
7224469 |
- |
Rbfox1 |
NNNGATACAGGTTTAATGTG |
NGG |
2 |
0.1538 |
Tier I |
| 2 |
NC_000077.6 |
11 |
85444421 |
+ |
Bcas3 |
NNNGATCCAGGTTTAATTCC |
NGG |
2 |
0.1875 |
Tier II |
| 3 |
NC_000077.6 |
11 |
106758427 |
+ |
Milr1 |
NNNGATCCAGGTTTAATTCC |
NGG |
2 |
0.1875 |
Tier II |
| 4 |
NC_000077.6 |
11 |
80442294 |
+ |
Psmd11 |
NNNGATACAGGTTTTATTCT |
NGG |
2 |
0.14 |
Tier II |
| 5 |
NC_000073.6 |
7 |
44617195 |
+ |
Myh14 |
NNNGATACAGGTTTAATTCC |
NAG |
2 |
0.1111 |
Tier II |
| 6 |
NC_000070.6 |
4 |
111121275 |
+ |
Agbl4 |
NNNGATACATGTTTAATTGG |
NGG |
2 |
0.1016 |
Tier II |
| 7 |
NC_000070.6 |
4 |
116650740 |
- |
Akr1a1 |
NNNGATACAGGTTTAATTCC |
NGT |
2 |
0.0069 |
Tier II |
Other clones with same target sequence:
(none)
