Construct: sgRNA BRDN0001146036
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCACCAGGCACGTTCACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP4K4 (9448)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76265
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
101844109 |
- |
MAP4K4 |
NNNACCAGGCACGTTCACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
123886301 |
- |
XIAP |
NNNACCAGGCACGATCACAA |
NGT |
2 |
0.01 |
Tier I |
| 3 |
NC_000024.10 |
Y |
7014314 |
- |
TBL1Y |
NNNACCAGGCACATTCACAT |
NGG |
2 |
0.5538 |
Tier II |
| 4 |
NC_000010.11 |
10 |
94954292 |
+ |
CYP2C9 |
NNNACCAGGCAAGTTCAAAA |
NGG |
2 |
0.3846 |
Tier II |
| 5 |
NC_000007.14 |
7 |
12532115 |
- |
LOC102725191 |
NNNACCAGGCATGTTCAAAA |
NGG |
2 |
0.2899 |
Tier II |
| 6 |
NC_000008.11 |
8 |
55260854 |
- |
XKR4 |
NNNACCAGGCATGTTCAAAA |
NGG |
2 |
0.2899 |
Tier II |
| 7 |
NC_000013.11 |
13 |
95982385 |
+ |
UGGT2 |
NNNACCAGGCATGTTCAAAA |
NGG |
2 |
0.2899 |
Tier II |
| 8 |
NC_000016.10 |
16 |
4597264 |
+ |
C16orf96 |
NNNACCAGGCAGGATCACAA |
NGG |
2 |
0.2751 |
Tier II |
| 9 |
NC_000001.11 |
1 |
174810073 |
- |
RABGAP1L |
NNNACCAGGCAGTTTCACAA |
NGG |
2 |
0.1333 |
Tier II |
| 10 |
NC_000009.12 |
9 |
91459172 |
- |
NFIL3 |
NNNACCAGGCAGGTCCACAA |
NGG |
2 |
0.1212 |
Tier II |
| 11 |
NC_000005.10 |
5 |
874543 |
+ |
BRD9 |
NNNACCAGGCACGTGCACAA |
NGG |
1 |
0.05 |
Tier II |
| 12 |
NC_000010.11 |
10 |
48724526 |
+ |
WDFY4 |
NNNACCAGGCACTTTCACAA |
NTG |
2 |
0.0117 |
Tier II |
| 13 |
NC_000006.12 |
6 |
119566808 |
+ |
LOC105377975 |
NNNACTAGGCATGTTCACAA |
NGG |
2 |
0.5 |
Tier III |
| 14 |
NC_000006.12 |
6 |
1554102 |
- |
LOC102723944 |
NNNACCAGGTACCTTCACAA |
NGG |
2 |
0.3963 |
Tier III |
| 15 |
NC_000013.11 |
13 |
59104420 |
- |
LOC105370221 |
NNNACCTGTCACGTTCACAA |
NGG |
2 |
0.2333 |
Tier III |
| 16 |
NC_000011.10 |
11 |
27188576 |
+ |
BBOX1-AS1 |
NNNAGCAGGGACGTTCACAA |
NGG |
2 |
0.2333 |
Tier III |
| 17 |
NC_000004.12 |
4 |
148904245 |
- |
LOC107986195 |
NNNACCAGGCACGTCCGCAA |
NGG |
2 |
0.0481 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
61211043 |
- |
Aldh3a1 |
NNNACCAAGCACATTCACAA |
NGG |
2 |
0.9231 |
Tier II |
| 2 |
NC_000068.7 |
2 |
179742772 |
+ |
Cdh4 |
NNNACCAGGCATGATCACAA |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000067.6 |
1 |
36633895 |
- |
Fam178b |
NNNACCAGGCAGGTACACAA |
NGG |
2 |
0.2573 |
Tier II |
| 4 |
NC_000083.6 |
17 |
42428239 |
- |
Ptchd4 |
NNNACCAGGCACATTCTCAA |
NGG |
2 |
0.1231 |
Tier II |
| 5 |
NC_000074.6 |
8 |
27233035 |
+ |
Gm45470 |
NNNGCCAGGCACGTTCACAA |
NTG |
2 |
0.0244 |
Tier III |
Other clones with same target sequence:
(none)
