Construct: sgRNA BRDN0001146043

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGAGATCCGATACTACGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA10 (284656)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76099

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 37735262 - EPHA10 NNNGATCCGATACTACGAGA NGG 0 1.0 Tier I
2 NC_000003.12 3 135154272 + EPHB1 NNNGATCCGGTACTATGAGA NGG 2 0.1709 Tier I
3 NC_000020.11 20 47226372 + ZMYND8 NNNGATCTGATACTACGTGA NGG 2 0.4375 Tier II
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 102010022 - Ephb1 NNNGATCCGGTACTATGAGA NGG 2 0.1709 Tier I
2 NC_000070.6 4 124899945 + Epha10 NNNGATCCGCTACTATGAGA NGG 2 0.1026 Tier I
Download CSV

Other clones with same target sequence:

(none)