Construct: sgRNA BRDN0001146044

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGTCCCCACTTACGTTCCGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK1D (57118)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77445

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 12349907 - CAMK1D NNNCCCCACTTACGTTCCGA NGG 0 1.0 Tier I
2 NC_000021.9 21 32580648 + TCP10L NNNCCCCACTTACGTTTGGA NGG 2 0.0622 Tier II
3 NC_000021.9 21 32580648 + CFAP298-TCP10L NNNCCCCACTTACGTTTGGA NGG 2 0.0622 Tier II
4 NC_000001.11 1 16632995 - CROCCP2 NNNCCCCACTGAGGTTCCGA NGG 2 0.0545 Tier III
5 NC_000001.11 1 21434686 + CROCCP5 NNNCCCCACTGAGGTTCCGA NGG 2 0.0545 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 5714150 + Camk1d NNNACCCACTTACGTTCCGA NGG 1 0.8421 Tier I
2 NC_000077.6 11 50779929 + Adamts2 NNNCCCCACTTAGGTTCAGA NGG 2 0.0734 Tier II
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Other clones with same target sequence:

(none)