Construct: sgRNA BRDN0001146046
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGCAGTACTGACATATCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKD3 (23683)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75952
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
37286238 |
+ |
PRKD3 |
NNNCAGTACTGACATATCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
59925771 |
- |
CYP2J2 |
NNNCAATACTGACATATAGT |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000002.12 |
2 |
165993984 |
+ |
SCN1A |
NNNCAATACTGACATATAGT |
NGG |
2 |
0.5385 |
Tier II |
| 4 |
NC_000001.11 |
1 |
7442810 |
- |
CAMTA1 |
NNNCAGTACTGACATACAGT |
NGG |
2 |
0.3801 |
Tier II |
| 5 |
NC_000001.11 |
1 |
46280690 |
- |
LRRC41 |
NNNCAGTGCTGACATATGGT |
NGG |
2 |
0.0978 |
Tier II |
| 6 |
NC_000001.11 |
1 |
155403347 |
- |
ASH1L |
NNNCAGTACTGACATATCTT |
NTG |
2 |
0.026 |
Tier II |
| 7 |
NC_000002.12 |
2 |
165993984 |
+ |
LOC102724058 |
NNNCAATACTGACATATAGT |
NGG |
2 |
0.5385 |
Tier III |
| 8 |
NC_000002.12 |
2 |
195086302 |
- |
LOC105376755 |
NNNCATTACTGACATATGGT |
NGG |
2 |
0.0889 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
78975436 |
+ |
Prkd3 |
NNNCAGTACTGGCATATGGT |
NGG |
2 |
0.0963 |
Tier I |
| 2 |
NC_000068.7 |
2 |
77575141 |
+ |
Zfp385b |
NNNCAGTACTGGCATATCCT |
NGG |
2 |
0.3238 |
Tier II |
| 3 |
NC_000070.6 |
4 |
106502327 |
+ |
Tmem61 |
NNNCAGTTCTGACATATCCT |
NGG |
2 |
0.1921 |
Tier II |
Other clones with same target sequence:
(none)
