Construct: sgRNA BRDN0001146050
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAGTTTGACCATCCACACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TYRO3 (7301)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76228
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
41571670 |
+ |
TYRO3 |
NNNGTTTGACCATCCACACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
138122070 |
+ |
CACNA1B |
NNNGTTTTATCATCCACACG |
NGG |
2 |
0.5882 |
Tier II |
| 3 |
NC_000015.10 |
15 |
76259973 |
- |
ETFA |
NNNGTTTGACCATCCACATG |
NGG |
1 |
0.4615 |
Tier II |
| 4 |
NC_000001.11 |
1 |
40655836 |
- |
RIMS3 |
NNNGTTTGACCATCCAGACG |
NGG |
1 |
0.0588 |
Tier II |
| 5 |
NC_000015.10 |
15 |
76259973 |
- |
TYRO3P |
NNNGTTTGACCATCCACATG |
NGG |
1 |
0.4615 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
119811673 |
+ |
Tyro3 |
NNNGTTTGACCATCCACACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000078.6 |
12 |
104696409 |
- |
Dicer1 |
NNNGTTTGACCATCCAGACG |
NAG |
2 |
0.0153 |
Tier I |
| 3 |
NC_000074.6 |
8 |
113794604 |
+ |
Adamts18 |
NNNATTTGTCCATCCACACG |
NGG |
2 |
0.54 |
Tier II |
| 4 |
NC_000078.6 |
12 |
31605336 |
+ |
Bcap29 |
NNNGTGTGACCACCCACACG |
NGG |
2 |
0.4511 |
Tier II |
| 5 |
NC_000076.6 |
10 |
64843826 |
- |
Ctnna3 |
NNNGAATGACCATCCACACG |
NGG |
2 |
0.4333 |
Tier II |
| 6 |
NC_000074.6 |
8 |
69847187 |
- |
Pbx4 |
NNNATTTGACCCTCCACACG |
NGG |
2 |
0.2368 |
Tier II |
| 7 |
NC_000083.6 |
17 |
40294797 |
+ |
Crisp1 |
NNNTTTTCACCATCCACACG |
NGG |
2 |
0.2238 |
Tier II |
| 8 |
NC_000069.6 |
3 |
103308148 |
- |
Trim33 |
NNNGTTTGACACTCCACACG |
NGG |
2 |
0.1974 |
Tier II |
| 9 |
NC_000080.6 |
14 |
22371376 |
- |
Lrmda |
NNNGTTTGACGTTCCACACG |
NGG |
2 |
0.0833 |
Tier II |
| 10 |
NC_000077.6 |
11 |
86770471 |
- |
Dhx40 |
NNNGGTTGACCATGCACACG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000077.6 |
11 |
86770471 |
- |
Gm42085 |
NNNGGTTGACCATGCACACG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
