Construct: sgRNA BRDN0001146051
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCATGTCAACCAAACATCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K4 (4216)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76513
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
161034404 |
+ |
MAP3K4 |
NNNTGTCAACCAAACATCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
44513104 |
+ |
NELL2 |
NNNTGTCAACCAAACATAAA |
NGG |
2 |
0.5048 |
Tier II |
| 3 |
NC_000002.12 |
2 |
45930702 |
- |
PRKCE |
NNNTGTCAAACAAACAACAG |
NGG |
2 |
0.4622 |
Tier II |
| 4 |
NC_000023.11 |
X |
8718717 |
- |
ANOS1 |
NNNTGGCAACCAGACATCAG |
NGG |
2 |
0.3727 |
Tier II |
| 5 |
NC_000003.12 |
3 |
52254717 |
+ |
WDR82 |
NNNTGTCAAATAAACATCAG |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000013.11 |
13 |
32279802 |
+ |
FRY |
NNNTTTCAACCAAACACCAG |
NGG |
2 |
0.2118 |
Tier II |
| 7 |
NC_000010.11 |
10 |
50841109 |
+ |
A1CF |
NNNTCTCAACCAAACAGCAG |
NGG |
2 |
0.0924 |
Tier II |
| 8 |
NC_000009.12 |
9 |
13044922 |
- |
LOC100130801 |
NNNTGGCAGCCAAACATCAG |
NGG |
2 |
0.381 |
Tier III |
| 9 |
NC_000004.12 |
4 |
177269610 |
+ |
LOC105377557 |
NNNTGTCAACCAAACATAAT |
NGG |
2 |
0.3769 |
Tier III |
| 10 |
NC_000003.12 |
3 |
102627532 |
+ |
LOC105374016 |
NNNTGTCATGCAAACATCAG |
NGG |
2 |
0.2333 |
Tier III |
| 11 |
NC_000005.10 |
5 |
54375238 |
- |
LINC01033 |
NNNTGTCATCCAAACATCAG |
NGA |
2 |
0.0417 |
Tier III |
| 12 |
NC_000009.12 |
9 |
62067087 |
- |
LOC107987008 |
NNNTGTCCACCAAACCTCAG |
NGG |
2 |
0.0 |
Tier III |
| 13 |
NC_000009.12 |
9 |
67521293 |
+ |
LOC105379452 |
NNNTGTCCACCAAACCTCAG |
NGG |
2 |
0.0 |
Tier III |
| 14 |
NC_000006.12 |
6 |
22698655 |
+ |
LINC03005 |
NNNTGTGAACCAAACCTCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
96998092 |
+ |
Dscam |
NNNTGTTAACAAAACATCAG |
NGG |
2 |
0.6094 |
Tier II |
| 2 |
NC_000076.6 |
10 |
20140745 |
- |
Map3k5 |
NNNCGACAACCAAACATCAG |
NGG |
2 |
0.5608 |
Tier II |
| 3 |
NC_000085.6 |
19 |
3709523 |
+ |
1810055G02Rik |
NNNTGTCAAACAAACATTAG |
NGG |
2 |
0.5571 |
Tier II |
| 4 |
NC_000073.6 |
7 |
118545126 |
- |
Tmc7 |
NNNTGTCAATCAAACAACAG |
NGG |
2 |
0.502 |
Tier II |
| 5 |
NC_000086.7 |
X |
145453014 |
- |
Amot |
NNNTGTCAACAAAACATCTG |
NGG |
2 |
0.4038 |
Tier II |
| 6 |
NC_000084.6 |
18 |
51143713 |
+ |
Prr16 |
NNNAGTCAACCAAACACCAG |
NGG |
2 |
0.3361 |
Tier II |
| 7 |
NC_000083.6 |
17 |
52608893 |
- |
Kcnh8 |
NNNTGTCCACCAAACATCAT |
NGG |
2 |
0.3 |
Tier II |
| 8 |
NC_000071.6 |
5 |
19356972 |
+ |
Magi2 |
NNNTGTCAACCAATCAACAG |
NGG |
2 |
0.2844 |
Tier II |
| 9 |
NC_000079.6 |
13 |
51150746 |
+ |
Spin1 |
NNNTGTCAAGCAAACACCAG |
NGG |
2 |
0.2745 |
Tier II |
| 10 |
NC_000076.6 |
10 |
107604361 |
+ |
Ptprq |
NNNTTTTAACCAAACATCAG |
NGG |
2 |
0.2438 |
Tier II |
| 11 |
NC_000083.6 |
17 |
42394901 |
+ |
Ptchd4 |
NNNTGTCAACCAAACGTCAA |
NGG |
2 |
0.1803 |
Tier II |
| 12 |
NC_000074.6 |
8 |
60535430 |
+ |
Aadat |
NNNTGTCAACTAAACATAAG |
NGG |
2 |
0.1657 |
Tier II |
| 13 |
NC_000078.6 |
12 |
52820814 |
- |
Akap6 |
NNNGGTCAACCACACATCAG |
NGG |
2 |
0.1316 |
Tier II |
| 14 |
NC_000073.6 |
7 |
50032702 |
- |
Nell1 |
NNNTGTCAACTAAACAGCAG |
NGG |
2 |
0.0362 |
Tier II |
| 15 |
NC_000077.6 |
11 |
9356430 |
- |
Abca13 |
NNNTGTCAATCAAACATCAG |
NGT |
2 |
0.0152 |
Tier II |
| 16 |
NC_000070.6 |
4 |
120518070 |
+ |
Scmh1 |
NNNTGTCAACCAAACATCTG |
NGC |
2 |
0.012 |
Tier II |
| 17 |
NC_000079.6 |
13 |
43062464 |
- |
Phactr1 |
NNNTGTCAACCAGACTTCAG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000074.6 |
8 |
17433261 |
+ |
C030002A05Rik |
NNNTGTCAACCCAACCTCAG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000074.6 |
8 |
17433261 |
+ |
Csmd1 |
NNNTGTCAACCCAACCTCAG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000073.6 |
7 |
61081684 |
- |
A230006K03Rik |
NNNTGTCAAACAAACATCAG |
NGG |
1 |
0.8667 |
Tier III |
| 21 |
NC_000080.6 |
14 |
53958070 |
+ |
Tcra |
NNNTCACAACCAAACATCAG |
NGG |
2 |
0.681 |
Tier III |
| 22 |
NC_000080.6 |
14 |
53958070 |
+ |
Tcrd |
NNNTCACAACCAAACATCAG |
NGG |
2 |
0.681 |
Tier III |
| 23 |
NC_000080.6 |
14 |
53958070 |
+ |
Gm30392 |
NNNTCACAACCAAACATCAG |
NGG |
2 |
0.681 |
Tier III |
| 24 |
NC_000076.6 |
10 |
107604361 |
+ |
Gm36177 |
NNNTTTTAACCAAACATCAG |
NGG |
2 |
0.2438 |
Tier III |
| 25 |
NC_000083.6 |
17 |
15199283 |
+ |
Gm35502 |
NNNTGTCAACAACACATCAG |
NGG |
2 |
0.1579 |
Tier III |
| 26 |
NC_000078.6 |
12 |
52820814 |
- |
Gm51971 |
NNNGGTCAACCACACATCAG |
NGG |
2 |
0.1316 |
Tier III |
| 27 |
NC_000071.6 |
5 |
87198917 |
- |
Gm19418 |
NNNTGTGAACCAAATATCAG |
NGG |
2 |
0.0314 |
Tier III |
Other clones with same target sequence:
(none)
