Construct: sgRNA BRDN0001146052
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAATTCTGTACCTTTCGAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NADK (65220)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78073
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
1756508 |
- |
NADK |
NNNTTCTGTACCTTTCGAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
189564099 |
- |
SLC40A1 |
NNNTTCCGTACCTTCCGAGA |
NGG |
2 |
0.1875 |
Tier I |
| 3 |
NC_000016.10 |
16 |
73617157 |
- |
ZFHX3 |
NNNTTCTGTACCTTTCAAGA |
NGG |
1 |
0.9333 |
Tier II |
| 4 |
NC_000012.12 |
12 |
2325802 |
+ |
CACNA1C |
NNNTTCTGTACCTTTCGTGA |
NGG |
1 |
0.5 |
Tier II |
| 5 |
NC_000002.12 |
2 |
30942466 |
+ |
GALNT14 |
NNNTTCTGTTCCTCTCGAGA |
NGG |
2 |
0.2521 |
Tier II |
| 6 |
NC_000015.10 |
15 |
98884723 |
+ |
IGF1R |
NNNTTCTGTACCCTTCCAGA |
NGG |
2 |
0.1858 |
Tier II |
| 7 |
NC_000011.10 |
11 |
83696678 |
+ |
DLG2 |
NNNTTCTCTACCTTCCGAGA |
NGG |
2 |
0.1678 |
Tier II |
| 8 |
NC_000011.10 |
11 |
94856990 |
+ |
AMOTL1 |
NNNTTCTGGACCTTTCCAGA |
NGG |
2 |
0.1457 |
Tier II |
| 9 |
NC_000018.10 |
18 |
68996543 |
+ |
CCDC102B |
NNNTTCTGTACCTTTGGAGA |
NGC |
2 |
0.0034 |
Tier II |
| 10 |
NC_000008.11 |
8 |
124848364 |
- |
LOC105375743 |
NNNTTCTGTACCTTTCATGA |
NGG |
2 |
0.4667 |
Tier III |
| 11 |
NC_000011.10 |
11 |
83696678 |
+ |
DLG2-AS2 |
NNNTTCTCTACCTTCCGAGA |
NGG |
2 |
0.1678 |
Tier III |
| 12 |
NC_000002.12 |
2 |
3047960 |
+ |
LINC01250 |
NNNTTCTGTACCTTTAGAGA |
NTG |
2 |
0.039 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
3353242 |
+ |
Xkr4 |
NNNTTCTGTACCTTTAAAGA |
NGG |
2 |
0.9333 |
Tier II |
| 2 |
NC_000071.6 |
5 |
21832122 |
+ |
Slc26a5 |
NNNTTCTGTAACTTTCGAGA |
NGG |
1 |
0.75 |
Tier II |
| 3 |
NC_000068.7 |
2 |
43850163 |
- |
Arhgap15 |
NNNTTCTGTACCCTTCGAAA |
NGG |
2 |
0.5639 |
Tier II |
| 4 |
NC_000075.6 |
9 |
35027621 |
+ |
Kirrel3 |
NNNTTCTGTACCTTTCGGGA |
NGG |
1 |
0.4 |
Tier II |
| 5 |
NC_000077.6 |
11 |
35348609 |
+ |
Slit3 |
NNNTTCTTTACCTTACGAGA |
NGG |
2 |
0.3618 |
Tier II |
| 6 |
NC_000072.6 |
6 |
85955657 |
- |
Dusp11 |
NNNTTCTGTATATTTCGAGA |
NGG |
2 |
0.2198 |
Tier II |
| 7 |
NC_000074.6 |
8 |
123509414 |
+ |
Dbndd1 |
NNNTGCTGTACCTTTTGAGA |
NGG |
2 |
0.1969 |
Tier II |
| 8 |
NC_000073.6 |
7 |
64644631 |
+ |
Apba2 |
NNNTTCTGTAGCTTTCCAGA |
NGG |
2 |
0.0588 |
Tier II |
| 9 |
NC_000081.6 |
15 |
6463816 |
+ |
C9 |
NNNTTCTGTAGCTTTCCAGA |
NGG |
2 |
0.0588 |
Tier II |
| 10 |
NC_000081.6 |
15 |
84216679 |
+ |
Gm52212 |
NNNTTCTGTCCCTTTCCAGA |
NGG |
2 |
0.0784 |
Tier III |
| 11 |
NC_000070.6 |
4 |
114856024 |
- |
Gm12830 |
NNNTTCTGTACCTTTTGAGC |
NGG |
2 |
0.0699 |
Tier III |
| 12 |
NC_000070.6 |
4 |
114856024 |
- |
Gm33655 |
NNNTTCTGTACCTTTTGAGC |
NGG |
2 |
0.0699 |
Tier III |
Other clones with same target sequence:
(none)
