Construct: sgRNA BRDN0001146057
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCATCGCTCACAACCAAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERBB2 (2064)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76376
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
39708366 |
+ |
ERBB2 |
NNNTCGCTCACAACCAAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
133466038 |
- |
FSTL4 |
NNNCCACTCACAACCAAGTG |
NGG |
2 |
0.6471 |
Tier II |
| 3 |
NC_000004.12 |
4 |
19318270 |
+ |
LINC02438 |
NNNTCGCTCACAACAAAGTG |
NAG |
2 |
0.0576 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
108035927 |
+ |
Gstm4 |
NNNGCACTCACAACCAAGTG |
NGG |
2 |
0.625 |
Tier II |
| 2 |
NC_000070.6 |
4 |
134247514 |
- |
Gm30191 |
NNNTCTCTCACAACCAAGTA |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000077.6 |
11 |
100689987 |
- |
Zfp385c |
NNNCCGCACACAACCAAGTG |
NGG |
2 |
0.5176 |
Tier II |
| 4 |
NC_000083.6 |
17 |
78593364 |
- |
Vit |
NNNTAGCTCAAAACCAAGTG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000071.6 |
5 |
17659102 |
- |
Sema3c |
NNNTTGCTCACAACCAGGTG |
NGG |
2 |
0.1123 |
Tier II |
| 6 |
NC_000077.6 |
11 |
116271507 |
- |
Srp68 |
NNNTGGCTCACAACCACGTG |
NGG |
2 |
0.1059 |
Tier II |
| 7 |
NC_000076.6 |
10 |
62618511 |
+ |
Ddx50 |
NNNTGGCTCACAACCATGTG |
NGG |
2 |
0.08 |
Tier II |
| 8 |
NC_000085.6 |
19 |
46066185 |
+ |
Pprc1 |
NNNTGGCTCACAACTAAGTG |
NGG |
2 |
0.04 |
Tier II |
| 9 |
NC_000074.6 |
8 |
87910760 |
- |
Zfp423 |
NNNTTGCTCACAAGCAAGTG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000069.6 |
3 |
108035927 |
+ |
Gm36498 |
NNNGCACTCACAACCAAGTG |
NGG |
2 |
0.625 |
Tier III |
| 11 |
NC_000077.6 |
11 |
100689987 |
- |
Gm38483 |
NNNCCGCACACAACCAAGTG |
NGG |
2 |
0.5176 |
Tier III |
| 12 |
NC_000072.6 |
6 |
140967131 |
- |
Gm30524 |
NNNTGGCTCACAACCAACTG |
NGG |
2 |
0.2857 |
Tier III |
Other clones with same target sequence:
(none)
