Construct: sgRNA BRDN0001146060
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATTCATACTAAACACTCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80244
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
37616178 |
+ |
RELL1 |
NNNTCATGCTAAGCACTCTA |
NGG |
2 |
0.4783 |
Tier II |
| 2 |
NC_000018.10 |
18 |
6277070 |
- |
L3MBTL4 |
NNNACATAATAAACACTCTA |
NGG |
2 |
0.4082 |
Tier II |
| 3 |
NC_000015.10 |
15 |
71561350 |
- |
THSD4 |
NNNTCATACCAAACACTTTA |
NGG |
2 |
0.3429 |
Tier II |
| 4 |
NC_000009.12 |
9 |
126498785 |
- |
MVB12B |
NNNTCATACTAAACACTCTA |
NAG |
1 |
0.2593 |
Tier II |
| 5 |
NC_000007.14 |
7 |
16563905 |
- |
LRRC72 |
NNNTGATACTAAAAACTCTA |
NGG |
2 |
0.21 |
Tier II |
| 6 |
NC_000004.12 |
4 |
47835173 |
- |
CORIN |
NNNTCACACTAAACACTCAA |
NGG |
2 |
0.1964 |
Tier II |
| 7 |
NC_000002.12 |
2 |
187530357 |
+ |
TFPI |
NNNGCATACTAAACATTCTA |
NGG |
2 |
0.1923 |
Tier II |
| 8 |
NC_000007.14 |
7 |
94575410 |
- |
CASD1 |
NNNTCATACCAAACACTCTC |
NGG |
2 |
0.1212 |
Tier II |
| 9 |
NC_000002.12 |
2 |
50151484 |
- |
NRXN1 |
NNNTCATATTAAACACTCTA |
NGA |
2 |
0.0608 |
Tier II |
| 10 |
NC_000011.10 |
11 |
47682344 |
- |
AGBL2 |
NNNTCATATTAAACACTCTA |
NGA |
2 |
0.0608 |
Tier II |
| 11 |
NC_000012.12 |
12 |
68648161 |
+ |
RAP1B |
NNNTCATACTAAACATTCTA |
NGT |
2 |
0.005 |
Tier II |
| 12 |
NC_000009.12 |
9 |
111402560 |
- |
ECPAS |
NNNTCATACTAAAGATTCTA |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000002.12 |
2 |
74379304 |
+ |
DCTN1 |
NNNTCATCCTAAAGACTCTA |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000004.12 |
4 |
131135451 |
- |
LOC105377422 |
NNNTTATACTAAACAATCTA |
NGG |
2 |
0.6364 |
Tier III |
| 15 |
NC_000004.12 |
4 |
47835173 |
- |
LOC101927179 |
NNNTCACACTAAACACTCAA |
NGG |
2 |
0.1964 |
Tier III |
| 16 |
NC_000002.12 |
2 |
187530357 |
+ |
CALCRL-AS1 |
NNNGCATACTAAACATTCTA |
NGG |
2 |
0.1923 |
Tier III |
| 17 |
NC_000003.12 |
3 |
34239260 |
- |
LINC01811 |
NNNTCATACTTACCACTCTA |
NGG |
2 |
0.0648 |
Tier III |
| 18 |
NC_000006.12 |
6 |
105626555 |
- |
LINC02836 |
NNNTCATGCTAAACACTCTA |
NTG |
2 |
0.0286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
50208896 |
+ |
Bbx |
NNNTCAAACTAAACACTATA |
NGG |
2 |
0.4712 |
Tier II |
| 2 |
NC_000078.6 |
12 |
71870074 |
- |
Daam1 |
NNNTCATACAAACCACTCTA |
NGG |
2 |
0.1805 |
Tier II |
| 3 |
NC_000067.6 |
1 |
123653899 |
+ |
Dpp10 |
NNNTCATACAAAACTCTCTA |
NGG |
2 |
0.1714 |
Tier II |
| 4 |
NC_000069.6 |
3 |
80869496 |
+ |
Glrb |
NNNTCATACTAAACACTCCT |
NGG |
2 |
0.1655 |
Tier II |
| 5 |
NC_000071.6 |
5 |
99488762 |
- |
A930011G23Rik |
NNNTCATCCTAACCACTCTA |
NGG |
2 |
0.0902 |
Tier III |
| 6 |
NC_000072.6 |
6 |
141895312 |
- |
Gm30843 |
NNNTCACACTAAACACTCTA |
NTG |
2 |
0.0268 |
Tier III |
Other clones with same target sequence:
(none)
