Construct: sgRNA BRDN0001146060

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATTCATACTAAACACTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80244

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 37616178 + RELL1 NNNTCATGCTAAGCACTCTA NGG 2 0.4783 Tier II
2 NC_000018.10 18 6277070 - L3MBTL4 NNNACATAATAAACACTCTA NGG 2 0.4082 Tier II
3 NC_000015.10 15 71561350 - THSD4 NNNTCATACCAAACACTTTA NGG 2 0.3429 Tier II
4 NC_000009.12 9 126498785 - MVB12B NNNTCATACTAAACACTCTA NAG 1 0.2593 Tier II
5 NC_000007.14 7 16563905 - LRRC72 NNNTGATACTAAAAACTCTA NGG 2 0.21 Tier II
6 NC_000004.12 4 47835173 - CORIN NNNTCACACTAAACACTCAA NGG 2 0.1964 Tier II
7 NC_000002.12 2 187530357 + TFPI NNNGCATACTAAACATTCTA NGG 2 0.1923 Tier II
8 NC_000007.14 7 94575410 - CASD1 NNNTCATACCAAACACTCTC NGG 2 0.1212 Tier II
9 NC_000002.12 2 50151484 - NRXN1 NNNTCATATTAAACACTCTA NGA 2 0.0608 Tier II
10 NC_000011.10 11 47682344 - AGBL2 NNNTCATATTAAACACTCTA NGA 2 0.0608 Tier II
11 NC_000012.12 12 68648161 + RAP1B NNNTCATACTAAACATTCTA NGT 2 0.005 Tier II
12 NC_000009.12 9 111402560 - ECPAS NNNTCATACTAAAGATTCTA NGG 2 0.0 Tier II
13 NC_000002.12 2 74379304 + DCTN1 NNNTCATCCTAAAGACTCTA NGG 2 0.0 Tier II
14 NC_000004.12 4 131135451 - LOC105377422 NNNTTATACTAAACAATCTA NGG 2 0.6364 Tier III
15 NC_000004.12 4 47835173 - LOC101927179 NNNTCACACTAAACACTCAA NGG 2 0.1964 Tier III
16 NC_000002.12 2 187530357 + CALCRL-AS1 NNNGCATACTAAACATTCTA NGG 2 0.1923 Tier III
17 NC_000003.12 3 34239260 - LINC01811 NNNTCATACTTACCACTCTA NGG 2 0.0648 Tier III
18 NC_000006.12 6 105626555 - LINC02836 NNNTCATGCTAAACACTCTA NTG 2 0.0286 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 50208896 + Bbx NNNTCAAACTAAACACTATA NGG 2 0.4712 Tier II
2 NC_000078.6 12 71870074 - Daam1 NNNTCATACAAACCACTCTA NGG 2 0.1805 Tier II
3 NC_000067.6 1 123653899 + Dpp10 NNNTCATACAAAACTCTCTA NGG 2 0.1714 Tier II
4 NC_000069.6 3 80869496 + Glrb NNNTCATACTAAACACTCCT NGG 2 0.1655 Tier II
5 NC_000071.6 5 99488762 - A930011G23Rik NNNTCATCCTAACCACTCTA NGG 2 0.0902 Tier III
6 NC_000072.6 6 141895312 - Gm30843 NNNTCACACTAAACACTCTA NTG 2 0.0268 Tier III
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Other clones with same target sequence:

(none)