Construct: sgRNA BRDN0001146062

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTCCGAAATTTACCGCATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGF1R (3480)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76691

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 98908822 + IGF1R NNNCGAAATTTACCGCATGG NGG 0 1.0 Tier I
2 NC_000023.11 X 29224597 - IL1RAPL1 NNNCAAAATTTACCGCATTG NGG 2 0.5778 Tier II
3 NC_000007.14 7 50388307 + IKZF1 NNNCGAAATTTAACGAATGG NGG 2 0.3846 Tier II
4 NC_000018.10 18 3101153 + MYOM1 NNNAGAAATTTACAGCATGG NGG 2 0.2947 Tier II
5 NC_000007.14 7 50388307 + LOC124901631 NNNCGAAATTTAACGAATGG NGG 2 0.3846 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 68183481 + Igf1r NNNCGAAATTTACCGCATGG NGG 0 1.0 Tier I
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Other clones with same target sequence:

(none)