Construct: sgRNA BRDN0001146064

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTGAAAAGTATGTAGACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRAK3 (11213)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77713

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 66203766 + IRAK3 NNNGAAAAGTATGTAGACCA NGG 0 1.0 Tier I
2 NC_000001.11 1 111241439 + CHI3L2 NNNGAAGAGTATGGAGACCA NGG 2 0.0 Tier I
3 NC_000012.12 12 96536368 + CFAP54 NNNAAAAAGTATGTAAACCA NGG 2 0.9 Tier II
4 NC_000008.11 8 98576254 - STK3 NNNGATAGGTATGTAGACCA NGG 2 0.5238 Tier II
5 NC_000010.11 10 73408361 + ANXA7 NNNGAAAAGTAGATAGACCA NGG 2 0.4615 Tier II
6 NC_000007.14 7 32286778 + PDE1C NNNGAATAGTATGTGGACCA NGG 2 0.2844 Tier II
7 NC_000010.11 10 18230441 + CACNB2 NNNGAAAAGTTTATAGACCA NGG 2 0.284 Tier II
8 NC_000023.11 X 29350175 - IL1RAPL1 NNNTAACAGTATGTAGACCA NGG 2 0.1591 Tier II
9 NC_000006.12 6 39198897 + KCNK5 NNNGCAAAGTATTTAGACCA NGG 2 0.15 Tier II
10 NC_000001.11 1 72164118 + NEGR1 NNNGAAAAGTATGCAGACTA NGG 2 0.1319 Tier II
11 NC_000020.11 20 36151735 + EPB41L1 NNNGGAAAGTATGTAGTCCA NGG 2 0.096 Tier II
12 NC_000017.11 17 35687471 - AP2B1 NNNGAAAAGTATGTAGATGA NGG 2 0.0804 Tier II
13 NC_000011.10 11 72377781 + CLPB NNNGAAAATTATGTAGAGCA NGG 2 0.0711 Tier II
14 NC_000022.11 22 32985244 + SYN3 NNNGCAAAGTATGTAGTCCA NGG 2 0.0667 Tier II
15 NC_000006.12 6 87011903 - HTR1E NNNGAAAGGTATGTAGACCA NTG 2 0.0286 Tier II
16 NC_000023.11 X 106841184 + TBC1D8B NNNTAAAAGTATGTAGACCA NGA 2 0.0253 Tier II
17 NC_000018.10 18 32368709 - GAREM1 NNNGAAAAGTATGTAGGGCA NGG 2 0.0235 Tier II
18 NC_000001.11 1 108799625 - STXBP3 NNNGAAAAGTATGGAGACCA NTG 2 0.0 Tier II
19 NC_000006.12 6 110852698 - AMD1 NNNGAAAAGTATGGAGACCA NAG 2 0.0 Tier II
20 NC_000004.12 4 72412178 + ADAMTS3 NNNGAAATGTATGGAGACCA NGG 2 0.0 Tier II
21 NC_000007.14 7 113486976 + LOC124901813 NNNGAAAAATATGTAGACCT NGG 2 0.3857 Tier III
22 NC_000018.10 18 56016633 - LINC03069 NNNGAAAAATATGTAGAACA NGG 2 0.3462 Tier III
23 NC_000018.10 18 56016633 - LINC03092 NNNGAAAAATATGTAGAACA NGG 2 0.3462 Tier III
24 NC_000001.11 1 194297692 - LOC105371670 NNNGAAAAATATGTAGCCCA NGG 2 0.1134 Tier III
25 NC_000001.11 1 194297692 - LOC107985242 NNNGAAAAATATGTAGCCCA NGG 2 0.1134 Tier III
26 NC_000020.11 20 36151735 + EPB41L1-AS1 NNNGGAAAGTATGTAGTCCA NGG 2 0.096 Tier III
27 NC_000015.10 15 25518972 + LINC02250 NNNGAAAAGTATGTGGACCA NGA 2 0.0451 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 120182654 - Irak3 NNNGAAAAGTACGTAAACCA NGG 2 0.9474 Tier I
2 NC_000069.6 3 62573303 + Gpr149 NNNGAAAAGCATGTAGACCA NGG 1 0.5333 Tier II
3 NC_000067.6 1 166635513 - Gm4847 NNNGAAAGGTATGTAGACCT NGG 2 0.44 Tier II
4 NC_000078.6 12 116298559 - Esyt2 NNNGAAAGCTATGTAGACCA NGG 2 0.3949 Tier II
5 NC_000082.6 16 21923090 + Map3k13 NNNGAAAAGTATGTGGAACA NGG 2 0.35 Tier II
6 NC_000075.6 9 94716610 - Slc9a9 NNNGAAAAGTGTGTAGAACA NGG 2 0.35 Tier II
7 NC_000085.6 19 11781558 - Stx3 NNNGAAATGTAAGTAGACCA NGG 2 0.3429 Tier II
8 NC_000067.6 1 177256794 - Akt3 NNNGAAAAGGATGTAGAACA NGG 2 0.2692 Tier II
9 NC_000082.6 16 22251139 + Tra2b NNNAAAAAGTATGCAGACCA NGG 2 0.2571 Tier II
10 NC_000070.6 4 140088421 + Igsf21 NNNGAATACTATGTAGACCA NGG 2 0.2356 Tier II
11 NC_000071.6 5 13441496 - Sema3a NNNGAAAAGTCTGTAGAACA NGG 2 0.2154 Tier II
12 NC_000083.6 17 89044474 - Fshr NNNGAAAAGAATGTAGACCC NGG 2 0.1948 Tier II
13 NC_000085.6 19 57046812 - Ablim1 NNNGAAAAGTATATAGACCA NCG 2 0.0989 Tier II
14 NC_000076.6 10 31573798 - Rnf217 NNNGAAAAGTATATAGACCA NGA 2 0.0641 Tier II
15 NC_000084.6 18 38616706 - Arhgap26 NNNGAAAACTATGTAGACCA NGC 2 0.012 Tier II
16 NC_000086.7 X 151172231 - Wnk3 NNNGAACAGTATGTAGACCA NGC 2 0.0097 Tier II
17 NC_000072.6 6 77492922 - Ctnna2 NNNGAAAAGAATGGAGACCA NGG 2 0.0 Tier II
18 NC_000077.6 11 11505795 - Spata48 NNNGAAAAGTATGGAGACAA NGG 2 0.0 Tier II
19 NC_000079.6 13 14274047 - Hecw1 NNNGAAAAGTATGTAGACCA NCC 2 0.0 Tier II
20 NC_000077.6 11 79401218 - Nf1 NNNGAAAAGTTTGGAGACCA NGG 2 0.0 Tier II
21 NC_000076.6 10 120182654 - Gm34545 NNNGAAAAGTACGTAAACCA NGG 2 0.9474 Tier III
22 NC_000068.7 2 6293430 + A230108P19Rik NNNGATATGTATGTAGACCA NGG 2 0.3061 Tier III
23 NC_000074.6 8 30124571 + Gm39154 NNNGACAAGTATGTAGAGCA NGG 2 0.0606 Tier III
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Other clones with same target sequence:

(none)