Construct: sgRNA BRDN0001146065

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGTATGAGAACATGCACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPKAPK3 (7867)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77241

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 50640470 + MAPKAPK3 NNNTATGAGAACATGCACCA NGG 0 1.0 Tier I
2 NC_000023.11 X 125194377 - TENM1 NNNTATGAGGACATGAACCA NGG 2 0.5556 Tier II
3 NC_000006.12 6 10589607 - GCNT2 NNNTCTGAGAACATGAACCA NGG 2 0.5 Tier II
4 NC_000007.14 7 133895866 + EXOC4 NNNGATGAGAACATGCATCA NGG 2 0.4018 Tier II
5 NC_000002.12 2 160128567 + ITGB6 NNNTATGAGACCATGAACCA NGG 2 0.4 Tier II
6 NC_000020.11 20 47105756 + EYA2 NNNTATGAAAACAAGCACCA NGG 2 0.398 Tier II
7 NC_000002.12 2 75138986 + TACR1 NNNCATGAGAACATGCAACA NGG 2 0.3484 Tier II
8 NC_000003.12 3 38511965 - EXOG NNNTATGAGTACATGCACCA NAG 2 0.2288 Tier II
9 NC_000006.12 6 160091504 - IGF2R NNNTATGAGCAGATGCACCA NGG 2 0.1481 Tier II
10 NC_000017.11 17 57541433 + MSI2 NNNTATGACAACATGCACCC NGG 2 0.1224 Tier II
11 NC_000016.10 16 50557251 + NKD1 NNNTTTGAGAACATGTACCA NGG 2 0.1119 Tier II
12 NC_000007.14 7 147081227 + CNTNAP2 NNNTATGAGAACATACACCA NCG 2 0.1008 Tier II
13 NC_000022.11 22 28799006 + XBP1 NNNTATCAGAACATTCACCA NGG 2 0.0982 Tier II
14 NC_000014.9 14 77686546 - ALKBH1 NNNTATGAGCACATTCACCA NGG 2 0.0476 Tier II
15 NC_000012.12 12 79301209 + SYT1 NNNTATGAGAACATGAACCA NTG 2 0.039 Tier II
16 NC_000005.10 5 38370958 - EGFLAM NNNTATGAGAACATACACCA NTG 2 0.0367 Tier II
17 NC_000023.11 X 97402484 - DIAPH2 NNNTATGAGAACATCCAGCA NGG 2 0.0364 Tier II
18 NC_000017.11 17 27787506 + NOS2 NNNTATGAGAACCTGCTCCA NGG 2 0.0281 Tier II
19 NC_000012.12 12 108132736 - WSCD2 NNNTATGAAAACATGCACCA NGC 2 0.0143 Tier II
20 NC_000002.12 2 47038912 + TTC7A NNNTATGAGAGCAGGCACCA NGG 2 0.0 Tier II
21 NC_000003.12 3 39794481 + LOC105377039 NNNAAAGAGAACATGCACCA NGG 2 0.4127 Tier III
22 NC_000008.11 8 20810943 + LOC105379315 NNNGATGAGGACATGCACCA NGG 2 0.3472 Tier III
23 NC_000010.11 10 87347621 - LINC00863 NNNTATGAGAACATGCAATA NGG 2 0.2485 Tier III
24 NC_000005.10 5 89773767 - LINC02161 NNNTAGGAGAACTTGCACCA NGG 2 0.1714 Tier III
25 NC_000008.11 8 81491366 + LOC101927118 NNNTATGAGAAAATGGACCA NGG 2 0.1099 Tier III
26 NC_000007.14 7 147081227 + CNTNAP2-AS1 NNNTATGAGAACATACACCA NCG 2 0.1008 Tier III
27 NC_000013.11 13 84327933 - LINC00333 NNNAATGAGAACATTCACCA NGG 2 0.068 Tier III
28 NC_000015.10 15 80388626 - ARNT2-DT NNNTATGAGAACATTCACCA NCG 2 0.0153 Tier III
29 NC_000012.12 12 127285275 + LOC124903050 NNNTATGAGAACATGCCCCA NTG 2 0.0069 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 107263661 - Mapkapk3 NNNTATGAGAATATGCACCA NGG 1 0.5385 Tier I
2 NC_000071.6 5 113306248 - Sgsm1 NNNGATGAGAACATGAACCA NGG 2 0.625 Tier II
3 NC_000070.6 4 36406909 - Lingo2 NNNTAAGAGAATATGCACCA NGG 2 0.4667 Tier II
4 NC_000078.6 12 87056346 - Tmem63c NNNTATGGGGACATGCACCA NGG 2 0.4074 Tier II
5 NC_000076.6 10 41373564 - Ak9 NNNTATGAGAACATGAACCA NAG 2 0.2593 Tier II
6 NC_000078.6 12 110790755 + Wdr20 NNNTATGAGGTCATGCACCA NGG 2 0.1709 Tier II
7 NC_000072.6 6 126139062 + Ntf3 NNNTATGGGAACATGCAGCA NGG 2 0.0978 Tier II
8 NC_000078.6 12 46727365 - Nova1 NNNTATGTGAACATGCACCC NGG 2 0.0974 Tier II
9 NC_000076.6 10 14145696 - Hivep2 NNNTATGAGAACAAGCAGCA NGG 2 0.0825 Tier II
10 NC_000072.6 6 28686158 + Snd1 NNNTCTGAGAACATGCACCA NGA 2 0.0347 Tier II
11 NC_000080.6 14 96177543 + Klhl1 NNNTATGTGAACATGCACCA NGA 2 0.0298 Tier II
12 NC_000068.7 2 106751735 + Mpped2 NNNTATGAGAACATGCACAA NGT 2 0.0069 Tier II
13 NC_000070.6 4 68318485 + Gm12911 NNNGATGAAAACATGCACCA NGG 2 0.4018 Tier III
14 NC_000078.6 12 73377581 - D830013O20Rik NNNAATGAGACCATGCACCA NGG 2 0.1905 Tier III
15 NC_000068.7 2 58425886 + LOC108168760 NNNTATGTGAACATGCAGCA NGG 2 0.0571 Tier III
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Other clones with same target sequence:

(none)