Construct: sgRNA BRDN0001146068
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAACTGCGACAGGATACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA6 (285220)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76249
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
97448687 |
+ |
EPHA6 |
NNNACTGCGACAGGATACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
110228694 |
+ |
RDX |
NNNACTGGGACAGAATACAG |
NGG |
2 |
0.4821 |
Tier II |
| 3 |
NC_000003.12 |
3 |
121074066 |
+ |
STXBP5L |
NNNACTGCAACAGGACACAG |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000024.10 |
Y |
13342660 |
- |
UTY |
NNNACTGCAACAGGACACAG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000005.10 |
5 |
72834429 |
+ |
TNPO1 |
NNNAGTGGGACAGGATACAG |
NGG |
2 |
0.3857 |
Tier II |
| 6 |
NC_000023.11 |
X |
86384199 |
+ |
DACH2 |
NNNACTGAGACAGGATAAAG |
NGG |
2 |
0.35 |
Tier II |
| 7 |
NC_000016.10 |
16 |
4738386 |
+ |
DNAAF8 |
NNNACTGCTACAGGCTACAG |
NGG |
2 |
0.1455 |
Tier II |
| 8 |
NC_000004.12 |
4 |
4396608 |
- |
NSG1 |
NNNTCTGCGACAGGATGCAG |
NGG |
2 |
0.1123 |
Tier II |
| 9 |
NC_000012.12 |
12 |
21838444 |
+ |
ABCC9 |
NNNACTGCTACAGGATTCAG |
NGG |
2 |
0.0711 |
Tier II |
| 10 |
NC_000001.11 |
1 |
108240240 |
- |
NBPF4 |
NNNACTGTGACAGGATACAG |
NGC |
2 |
0.0194 |
Tier II |
| 11 |
NC_000001.11 |
1 |
108454106 |
+ |
NBPF6 |
NNNACTGTGACAGGATACAG |
NGC |
2 |
0.0194 |
Tier II |
| 12 |
NC_000008.11 |
8 |
93380364 |
+ |
CIBAR1-DT |
NNNACTGGGACAAGATACAG |
NGG |
2 |
0.5934 |
Tier III |
| 13 |
NC_000008.11 |
8 |
100352952 |
- |
LOC124901991 |
NNNACTGAGGCAGGATACAG |
NGG |
2 |
0.3611 |
Tier III |
| 14 |
NC_000004.12 |
4 |
19607977 |
- |
LOC105374511 |
NNNACTGCGACAGGACAAAG |
NGG |
2 |
0.359 |
Tier III |
| 15 |
NC_000001.11 |
1 |
108379645 |
+ |
NBPF5P |
NNNACTGTGACAGGATACAG |
NGC |
2 |
0.0194 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
37900669 |
+ |
Diaph1 |
NNNGCTGCGGCAGGATACAG |
NGG |
2 |
0.3472 |
Tier I |
| 2 |
NC_000082.6 |
16 |
60005581 |
- |
Epha6 |
NNNACGGCGACAGGCTACAG |
NGG |
2 |
0.1558 |
Tier I |
| 3 |
NC_000075.6 |
9 |
34981104 |
- |
Kirrel3 |
NNNACTTCGACAGGATACAG |
NGG |
1 |
0.5714 |
Tier II |
| 4 |
NC_000076.6 |
10 |
71378841 |
- |
Ipmk |
NNNACTGCGACAGGACACAC |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000070.6 |
4 |
18852855 |
- |
Cnbd1 |
NNNACTGAGACAGGATCCAG |
NGG |
2 |
0.1147 |
Tier II |
| 6 |
NC_000084.6 |
18 |
14893033 |
- |
Taf4b |
NNNACTGCAACAGGATGCAG |
NGG |
2 |
0.1134 |
Tier II |
| 7 |
NC_000086.7 |
X |
168353185 |
+ |
Frmpd4 |
NNNACTTCGACAGGATCCAG |
NGG |
2 |
0.1008 |
Tier II |
| 8 |
NC_000072.6 |
6 |
90734408 |
- |
Iqsec1 |
NNNACTGTGACAGGATACAG |
NCG |
2 |
0.0937 |
Tier II |
| 9 |
NC_000075.6 |
9 |
86772786 |
+ |
Snap91 |
NNNACTGAGACAGGATAGAG |
NGG |
2 |
0.0867 |
Tier II |
| 10 |
NC_000071.6 |
5 |
128000941 |
+ |
Tmem132d |
NNNACTGAGACAGGATACAG |
NTG |
2 |
0.0253 |
Tier II |
| 11 |
NC_000078.6 |
12 |
98712938 |
+ |
Ptpn21 |
NNNACTGCGACAGGATAAAG |
NGC |
2 |
0.012 |
Tier II |
| 12 |
NC_000075.6 |
9 |
34981104 |
- |
Kirrel3os |
NNNACTTCGACAGGATACAG |
NGG |
1 |
0.5714 |
Tier III |
| 13 |
NC_000069.6 |
3 |
21753841 |
- |
7530428D23Rik |
NNNACGGCGACAGGCTACAG |
NGG |
2 |
0.1558 |
Tier III |
| 14 |
NC_000072.6 |
6 |
68019522 |
+ |
Igk |
NNNACTTCGACAGGATCCAG |
NGG |
2 |
0.1008 |
Tier III |
Other clones with same target sequence:
(none)
