Construct: sgRNA BRDN0001146073
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGGAATGATAGATCGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP6 (51678)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76775
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
24650556 |
+ |
PALS2 |
NNNGGAATGATAGATCGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
8430653 |
- |
STK33 |
NNNAGAATGATAGATCAACA |
NGG |
2 |
0.84 |
Tier II |
| 3 |
NC_000004.12 |
4 |
113352899 |
- |
ANK2 |
NNNGGAATGGTAGATCCACA |
NGG |
2 |
0.1307 |
Tier II |
| 4 |
NC_000005.10 |
5 |
59315597 |
+ |
PDE4D |
NNNGGAATGATAGATGGTCA |
NGG |
2 |
0.0769 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
50163493 |
+ |
Mpp6 |
NNNGGAATGATAGATCGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000086.7 |
X |
162428633 |
+ |
Reps2 |
NNNGGAATGATAAATCAACA |
NGG |
2 |
0.8615 |
Tier II |
| 3 |
NC_000077.6 |
11 |
42585950 |
+ |
Gabrb2 |
NNNGGGATGATAGATAGACA |
NGG |
2 |
0.7143 |
Tier II |
| 4 |
NC_000077.6 |
11 |
50687723 |
- |
Adamts2 |
NNNGGATTGATAGAACGACA |
NGG |
2 |
0.2533 |
Tier II |
| 5 |
NC_000071.6 |
5 |
150430122 |
+ |
Fry |
NNNGGAATGATAGGTCGGCA |
NGG |
2 |
0.1867 |
Tier II |
Other clones with same target sequence:
(none)
