Construct: sgRNA BRDN0001146075
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGACACAAGGCACCTCGTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IP6K2 (51447)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77816
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
48689680 |
+ |
IP6K2 |
NNNCACAAGGCACCTCGTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
35132959 |
- |
NLE1 |
NNNCACAGGACACCTCGTAG |
NGG |
2 |
0.6844 |
Tier II |
| 3 |
NC_000002.12 |
2 |
239064839 |
- |
HDAC4 |
NNNCACAGGGCACCTCGCAG |
NGG |
2 |
0.3143 |
Tier II |
| 4 |
NC_000019.10 |
19 |
54994057 |
- |
NLRP2 |
NNNCAAAAGGGACCTCGTAG |
NGG |
2 |
0.2321 |
Tier II |
| 5 |
NC_000001.11 |
1 |
41867723 |
- |
HIVEP3 |
NNNCAGATGGCACCTCGTAG |
NGG |
2 |
0.2143 |
Tier II |
| 6 |
NC_000009.12 |
9 |
114308419 |
- |
COL27A1 |
NNNCACAAGGCATCTCCTAG |
NGG |
2 |
0.1647 |
Tier II |
| 7 |
NC_000003.12 |
3 |
183085756 |
- |
MCCC1 |
NNNCACAAGGCACCACGTAG |
NAG |
2 |
0.1501 |
Tier II |
| 8 |
NC_000004.12 |
4 |
10083929 |
+ |
WDR1 |
NNNCACAAGGCACCTCGCAG |
NAG |
2 |
0.1111 |
Tier II |
| 9 |
NC_000002.12 |
2 |
39388827 |
+ |
MAP4K3 |
NNNCACAAGGCACCTGGCAG |
NGG |
2 |
0.0659 |
Tier II |
| 10 |
NC_000009.12 |
9 |
114308419 |
- |
LOC105376224 |
NNNCACAAGGCATCTCCTAG |
NGG |
2 |
0.1647 |
Tier III |
| 11 |
NC_000006.12 |
6 |
96286886 |
+ |
UFL1-AS1 |
NNNCACAAGGCACCTCGCAG |
NAG |
2 |
0.1111 |
Tier III |
| 12 |
NC_000006.12 |
6 |
96340376 |
- |
UFL1-AS1 |
NNNCACAAGGCACGTGGTAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
108804576 |
- |
Ip6k2 |
NNNCACAAGGTACCTCATAG |
NGG |
2 |
0.2872 |
Tier I |
| 2 |
NC_000070.6 |
4 |
154927252 |
+ |
Tnfrsf14 |
NNNCACAAGGCACCTGGGAG |
NGG |
2 |
0.0513 |
Tier I |
| 3 |
NC_000070.6 |
4 |
10993638 |
- |
Plekhf2 |
NNNCACAAGTCACCTCGTAT |
NGG |
2 |
0.5688 |
Tier II |
| 4 |
NC_000073.6 |
7 |
44579652 |
+ |
Napsa |
NNNCACAAGGCACCTCACAG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000069.6 |
3 |
29807128 |
+ |
Gm33206 |
NNNCACAAGGCACCTCCTTG |
NGG |
2 |
0.1267 |
Tier III |
Other clones with same target sequence:
(none)
