Construct: sgRNA BRDN0001146092
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAACAAGTACTATGGCCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IP6K1 (9807)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76138
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
49727605 |
- |
IP6K1 |
NNNACAAGTACTATGGCCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
37140793 |
- |
CPLANE1 |
NNNACAGGTACTGTGGCCGT |
NGG |
2 |
0.4604 |
Tier II |
| 3 |
NC_000015.10 |
15 |
88126468 |
- |
NTRK3 |
NNNACAATTACTATGGCAGT |
NGG |
2 |
0.3365 |
Tier II |
| 4 |
NC_000023.11 |
X |
154350632 |
+ |
FLNA |
NNNACAAGTACCATGGCCGT |
NGC |
2 |
0.0211 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
108045514 |
+ |
Ip6k1 |
NNNATAAGTACTATGGCCGT |
NGA |
2 |
0.0442 |
Tier I |
| 2 |
NC_000067.6 |
1 |
68956302 |
+ |
Erbb4 |
NNNTCAAGTACTATGGCCAT |
NGG |
2 |
0.4545 |
Tier II |
| 3 |
NC_000080.6 |
14 |
52652876 |
- |
Tcra |
NNNACAAGTACTATGGCCTT |
NGG |
1 |
0.6667 |
Tier III |
| 4 |
NC_000067.6 |
1 |
68956302 |
+ |
Gm51744 |
NNNTCAAGTACTATGGCCAT |
NGG |
2 |
0.4545 |
Tier III |
Other clones with same target sequence:
(none)
