Construct: sgRNA BRDN0001146093
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCCAAACAGCCGATTCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDK2 (5164)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75686
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
50108180 |
+ |
PDK2 |
NNNCAAACAGCCGATTCACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
24546965 |
+ |
GUCD1 |
NNNCAAACAGCTGATTCACC |
NGG |
2 |
0.1224 |
Tier I |
| 3 |
NC_000007.14 |
7 |
16117201 |
- |
CRPPA |
NNNGAAACAACCGATTCACA |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000009.12 |
9 |
20255443 |
- |
SLC24A2 |
NNNCAAACTGCAGATTCACA |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000011.10 |
11 |
104104859 |
- |
PDGFD |
NNNCAAACAGCTGTTTCACA |
NGG |
2 |
0.2872 |
Tier II |
| 6 |
NC_000011.10 |
11 |
75942478 |
+ |
UVRAG |
NNNCAAACAGCAGATTCACA |
NCG |
2 |
0.0765 |
Tier II |
| 7 |
NC_000003.12 |
3 |
29827786 |
+ |
RBMS3 |
NNNCAAACAGCCTATTGACA |
NGG |
2 |
0.0176 |
Tier II |
| 8 |
NC_000006.12 |
6 |
16292065 |
- |
GMPR |
NNNCAACCAGCCGATTCACA |
NGC |
2 |
0.0097 |
Tier II |
| 9 |
NC_000003.12 |
3 |
81936110 |
+ |
LOC105377179 |
NNNCAAACAGCAGATCCACA |
NGG |
2 |
0.4762 |
Tier III |
| 10 |
NC_000010.11 |
10 |
18811747 |
+ |
LOC105376440 |
NNNCAAACAGCCAATTCACA |
NAG |
2 |
0.2393 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
95028975 |
- |
Pdk2 |
NNNCAACCAGCCCATTCACA |
NGG |
2 |
0.1842 |
Tier I |
| 2 |
NC_000069.6 |
3 |
118985761 |
+ |
Dpyd |
NNNCAAACAACTGATTCACA |
NGG |
2 |
0.5026 |
Tier II |
| 3 |
NC_000078.6 |
12 |
83435629 |
- |
Dpf3 |
NNNCAAACAACCGTTTCACA |
NGG |
2 |
0.4978 |
Tier II |
| 4 |
NC_000083.6 |
17 |
51153010 |
+ |
Tbc1d5 |
NNNCAAACAACCGTTTCACA |
NGG |
2 |
0.4978 |
Tier II |
| 5 |
NC_000079.6 |
13 |
8275576 |
- |
Adarb2 |
NNNCAAACAGCAGTTTCACA |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000082.6 |
16 |
91766345 |
- |
Itsn1 |
NNNCAAACAGCTGATTCATA |
NGG |
2 |
0.2485 |
Tier II |
| 7 |
NC_000067.6 |
1 |
40621758 |
- |
Slc9a4 |
NNNCAAACAGCCCTTTCACA |
NGG |
2 |
0.2246 |
Tier II |
| 8 |
NC_000067.6 |
1 |
125853613 |
+ |
Gpr39 |
NNNCAAACAGCCCTTTCACA |
NGG |
2 |
0.2246 |
Tier II |
| 9 |
NC_000068.7 |
2 |
28843988 |
- |
Ddx31 |
NNNCAAACAGCCCTTTCACA |
NGG |
2 |
0.2246 |
Tier II |
| 10 |
NC_000073.6 |
7 |
120642266 |
+ |
Uqcrc2 |
NNNCAAACAGCCCTTTCACA |
NGG |
2 |
0.2246 |
Tier II |
| 11 |
NC_000073.6 |
7 |
122001457 |
+ |
Gga2 |
NNNCAAACAGCCCTTTCACA |
NGG |
2 |
0.2246 |
Tier II |
| 12 |
NC_000076.6 |
10 |
85139880 |
+ |
Cry1 |
NNNCAAACAGCCCTTTCACA |
NGG |
2 |
0.2246 |
Tier II |
| 13 |
NC_000081.6 |
15 |
8643734 |
+ |
Slc1a3 |
NNNCAAACAGCCCTTTCACA |
NGG |
2 |
0.2246 |
Tier II |
| 14 |
NC_000077.6 |
11 |
88526384 |
- |
Msi2 |
NNNCAAACAGCCTTTTCACA |
NGG |
2 |
0.16 |
Tier II |
| 15 |
NC_000080.6 |
14 |
121920832 |
- |
Ubac2 |
NNNCACACAGCCGACTCACA |
NGG |
2 |
0.124 |
Tier II |
| 16 |
NC_000072.6 |
6 |
88804880 |
- |
Mgll |
NNNCAAACAGCCCCTTCACA |
NGG |
2 |
0.0902 |
Tier II |
| 17 |
NC_000077.6 |
11 |
67360955 |
- |
Myh13 |
NNNCAAACAGCCGTGTCACA |
NGG |
2 |
0.0267 |
Tier II |
| 18 |
NC_000072.6 |
6 |
15743819 |
- |
Mdfic |
NNNCAAACAGCCGCTTCACA |
NTG |
2 |
0.0083 |
Tier II |
| 19 |
NC_000074.6 |
8 |
71046136 |
+ |
Gm17576 |
NNNCAAATAGGCGATTCACA |
NGG |
2 |
0.2188 |
Tier III |
| 20 |
NC_000080.6 |
14 |
121920832 |
- |
Gm46485 |
NNNCACACAGCCGACTCACA |
NGG |
2 |
0.124 |
Tier III |
| 21 |
NC_000074.6 |
8 |
45120606 |
- |
Gm30504 |
NNNCAAACAACCGATTCACA |
NGA |
2 |
0.0648 |
Tier III |
| 22 |
NC_000073.6 |
7 |
126231164 |
+ |
1700123J17Rik |
NNNCAAACAGCAGATTCACA |
NGC |
2 |
0.0159 |
Tier III |
Other clones with same target sequence:
(none)
