Construct: sgRNA BRDN0001146094
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAAGTCCGTGAGCTTAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKX (5613)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78016
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
3655191 |
+ |
PRKX |
NNNAGTCCGTGAGCTTAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
170284569 |
- |
PRKCI |
NNNAGTCAGTGAGTTTAATG |
NGG |
2 |
0.4767 |
Tier I |
| 3 |
NC_000019.10 |
19 |
18134606 |
- |
MAST3 |
NNNAGTCCGTGAGCTTGATG |
NGG |
1 |
0.1765 |
Tier I |
| 4 |
NC_000001.11 |
1 |
46027758 |
- |
MAST2 |
NNNAGTCCGTGAGCTTGATG |
NGC |
2 |
0.0039 |
Tier I |
| 5 |
NC_000001.11 |
1 |
113392528 |
+ |
MAGI3 |
NNNAGTCCTTGAGCTTAATG |
NGG |
1 |
0.5333 |
Tier II |
| 6 |
NC_000018.10 |
18 |
65773734 |
+ |
CDH7 |
NNNAGTTAGTGAGCTTAATG |
NGG |
2 |
0.5281 |
Tier II |
| 7 |
NC_000008.11 |
8 |
61468770 |
- |
CLVS1 |
NNNAGTCCATGAGCTGAATG |
NGG |
2 |
0.2225 |
Tier II |
| 8 |
NC_000005.10 |
5 |
154785646 |
+ |
LARP1 |
NNNAGTCCATGTGCTTAATG |
NGG |
2 |
0.2143 |
Tier II |
| 9 |
NC_000008.11 |
8 |
79933626 |
+ |
MRPS28 |
NNNAGTCAGTGATCTTAATG |
NGG |
2 |
0.195 |
Tier II |
| 10 |
NC_000008.11 |
8 |
79933626 |
+ |
TPD52-MRPS28 |
NNNAGTCAGTGATCTTAATG |
NGG |
2 |
0.195 |
Tier II |
| 11 |
NC_000001.11 |
1 |
64499566 |
+ |
CACHD1 |
NNNAGTCCGTGAACTTGATG |
NGG |
2 |
0.1629 |
Tier II |
| 12 |
NC_000011.10 |
11 |
70116683 |
+ |
ANO1 |
NNNAGTCCGTGAGCTTTGTG |
NGG |
2 |
0.0533 |
Tier II |
| 13 |
NC_000023.11 |
X |
101538097 |
+ |
PRKCIP1 |
NNNAGTCAGTGAGTTTAATG |
NGG |
2 |
0.4767 |
Tier III |
| 14 |
NC_000010.11 |
10 |
121175248 |
- |
LOC124902515 |
NNNAGTCTGTGATCTTAATG |
NGG |
2 |
0.2625 |
Tier III |
| 15 |
NC_000024.10 |
Y |
7326128 |
- |
PRKY |
NNNAGTCCGTGAGCTTGATG |
NGA |
2 |
0.0123 |
Tier III |
| 16 |
NC_000015.10 |
15 |
100554085 |
+ |
PRKXP1 |
NNNAGTCCGTGAGCTTGATG |
NGT |
2 |
0.0028 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
81190429 |
- |
Dapk3 |
NNNAGTCGATGAGCTTAATG |
NGG |
2 |
0.4133 |
Tier I |
| 2 |
NC_000074.6 |
8 |
84921206 |
+ |
Mast1 |
NNNAATCCGTGAGCTTGATG |
NGG |
2 |
0.1529 |
Tier I |
| 3 |
NC_000070.6 |
4 |
155271497 |
+ |
Prkcz |
NNNAGTCCGTCAGCTTAATG |
NGT |
2 |
0.0069 |
Tier I |
| 4 |
NC_000087.7 |
Y |
1208521 |
- |
Uty |
NNNAGTCAGAGAGCTTAATG |
NGG |
2 |
0.5571 |
Tier II |
| 5 |
NC_000074.6 |
8 |
93000866 |
- |
Slc6a2 |
NNNAGTGAGTGAGCTTAATG |
NGG |
2 |
0.3059 |
Tier II |
| 6 |
NC_000083.6 |
17 |
84736730 |
- |
Lrpprc |
NNNAGACCGTGAGATTAATG |
NGG |
2 |
0.3033 |
Tier II |
| 7 |
NC_000084.6 |
18 |
84804871 |
- |
Gm17266 |
NNNAGTCCGTGAGCATATTG |
NGG |
2 |
0.2895 |
Tier II |
| 8 |
NC_000071.6 |
5 |
89714379 |
- |
Adamts3 |
NNNAGTCCCTGAGCTTACTG |
NGG |
2 |
0.1026 |
Tier II |
| 9 |
NC_000075.6 |
9 |
60393265 |
+ |
Thsd4 |
NNNAGTCCGTGAGCTCAATG |
NGA |
2 |
0.0463 |
Tier II |
| 10 |
NC_000073.6 |
7 |
27883343 |
+ |
Zfp607a |
NNNAGTCCGTCAGCTTAATG |
NGT |
2 |
0.0069 |
Tier II |
| 11 |
NC_000074.6 |
8 |
84921206 |
+ |
Gm38426 |
NNNAATCCGTGAGCTTGATG |
NGG |
2 |
0.1529 |
Tier III |
| 12 |
NC_000073.6 |
7 |
27883343 |
+ |
Prkcz2 |
NNNAGTCCGTCAGCTTAATG |
NGT |
2 |
0.0069 |
Tier III |
Other clones with same target sequence:
(none)
