Construct: sgRNA BRDN0001146095
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGCCATGGCTCCTACGGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PKMYT1 (9088)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77278
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
2976678 |
- |
PKMYT1 |
NNNCCATGGCTCCTACGGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
46460190 |
+ |
LRRC37A2 |
NNNCCATGGCCCCTAAGGAG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000017.11 |
17 |
46677881 |
+ |
NSF |
NNNCCATGGCCCCTAAGGAG |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000017.11 |
17 |
46677881 |
+ |
LRRC37A2 |
NNNCCATGGCCCCTAAGGAG |
NGG |
2 |
0.6667 |
Tier II |
| 5 |
NC_000011.10 |
11 |
89499185 |
- |
NOX4 |
NNNCCATGGCTCCTACAAAG |
NGG |
2 |
0.6462 |
Tier II |
| 6 |
NC_000002.12 |
2 |
214998793 |
- |
ABCA12 |
NNNCCATGGCTCCTAAGGTG |
NGG |
2 |
0.5385 |
Tier II |
| 7 |
NC_000022.11 |
22 |
29210194 |
+ |
EMID1 |
NNNCCATGTCTCCTAAGGAG |
NGG |
2 |
0.5333 |
Tier II |
| 8 |
NC_000018.10 |
18 |
6953224 |
- |
LAMA1 |
NNNCCATGGCTCCTATGGTG |
NGG |
2 |
0.1657 |
Tier II |
| 9 |
NC_000003.12 |
3 |
33426294 |
+ |
UBP1 |
NNNCAATGGCTCCTACTGAG |
NGG |
2 |
0.1429 |
Tier II |
| 10 |
NC_000012.12 |
12 |
1469428 |
+ |
ERC1 |
NNNCCTTGGCTCCTTCGGAG |
NGG |
2 |
0.1429 |
Tier II |
| 11 |
NC_000004.12 |
4 |
37270521 |
+ |
NWD2 |
NNNGCATGGCTCCCACGGAG |
NGG |
2 |
0.1429 |
Tier II |
| 12 |
NC_000017.11 |
17 |
46743092 |
+ |
NSF |
NNNCCATGACTCCTTCGGAG |
NGG |
2 |
0.1286 |
Tier II |
| 13 |
NC_000017.11 |
17 |
46743092 |
+ |
LRRC37A2 |
NNNCCATGACTCCTTCGGAG |
NGG |
2 |
0.1286 |
Tier II |
| 14 |
NC_000011.10 |
11 |
89499185 |
- |
H3P34 |
NNNCCATGGCTCCTACAAAG |
NGG |
2 |
0.6462 |
Tier III |
| 15 |
NC_000012.12 |
12 |
46474385 |
- |
SLC38A4-AS1 |
NNNCCATTGCTCCTGCGGAG |
NGG |
2 |
0.4063 |
Tier III |
| 16 |
NC_000012.12 |
12 |
46474385 |
- |
LOC124902923 |
NNNCCATTGCTCCTGCGGAG |
NGG |
2 |
0.4063 |
Tier III |
| 17 |
NC_000009.12 |
9 |
129438155 |
+ |
LOC105376291 |
NNNCCATGGCACCTCCGGAG |
NGG |
2 |
0.2045 |
Tier III |
| 18 |
NC_000008.11 |
8 |
102788963 |
+ |
LOC124901999 |
NNNCCAGGGCTCCTAGGGAG |
NGG |
2 |
0.0905 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
90917997 |
+ |
Ptpmt1 |
NNNCCATGGCTCCAAAGGAG |
NGG |
2 |
0.619 |
Tier I |
| 2 |
NC_000069.6 |
3 |
59183129 |
+ |
Gpr87 |
NNNCCATGGCTCCTAAGGAG |
NGG |
1 |
1.0 |
Tier II |
| 3 |
NC_000069.6 |
3 |
59183129 |
+ |
Med12l |
NNNCCATGGCTCCTAAGGAG |
NGG |
1 |
1.0 |
Tier II |
| 4 |
NC_000068.7 |
2 |
158209998 |
+ |
D630003M21Rik |
NNNCAATGGCTCCTGCGGAG |
NGG |
2 |
0.3714 |
Tier II |
| 5 |
NC_000070.6 |
4 |
99198879 |
+ |
Atg4c |
NNNCCAAGGCTCCTAGGGAG |
NGG |
2 |
0.1346 |
Tier II |
| 6 |
NC_000072.6 |
6 |
115273812 |
- |
Syn2 |
NNNCCATGGCTCTTAGGGAG |
NGG |
2 |
0.1077 |
Tier II |
| 7 |
NC_000079.6 |
13 |
20575361 |
- |
Elmo1 |
NNNCCATGGCTCCTACGCAG |
NGA |
2 |
0.0331 |
Tier II |
| 8 |
NC_000069.6 |
3 |
81118578 |
+ |
Pdgfc |
NNNCCATGGCTCCTAGGGAG |
NTG |
2 |
0.006 |
Tier II |
| 9 |
NC_000075.6 |
9 |
102819977 |
+ |
Gm18655 |
NNNTCATGGCTCCTGCGGAG |
NGG |
2 |
0.52 |
Tier III |
| 10 |
NC_000084.6 |
18 |
29657770 |
+ |
Gm7917 |
NNNCCATGGCTCCAGCGGAG |
NGG |
2 |
0.4024 |
Tier III |
Other clones with same target sequence:
(none)
