Construct: sgRNA BRDN0001146096
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGACGAAAATAGTTGTCCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TPK1 (27010)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77428
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
144765911 |
+ |
TPK1 |
NNNCGAAAATAGTTGTCCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
139476049 |
- |
RAB33B |
NNNAGAAAATAATTGTCCAA |
NGG |
2 |
0.786 |
Tier II |
| 3 |
NC_000015.10 |
15 |
29899820 |
+ |
TJP1 |
NNNACAAAATAGTTGTCCAA |
NGG |
2 |
0.6617 |
Tier II |
| 4 |
NC_000009.12 |
9 |
20930515 |
+ |
FOCAD |
NNNAGAAATTAGTTGTCCAA |
NGG |
2 |
0.5053 |
Tier II |
| 5 |
NC_000010.11 |
10 |
88451005 |
+ |
RNLS |
NNNGGAAAAAAGTTGTCCAA |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000001.11 |
1 |
53510320 |
- |
GLIS1 |
NNNCCAAAATAGTTGGCCAA |
NGG |
2 |
0.272 |
Tier II |
| 7 |
NC_000015.10 |
15 |
93027099 |
- |
CHD2 |
NNNCTAAAATAGTTGTCTAA |
NGG |
2 |
0.1929 |
Tier II |
| 8 |
NC_000008.11 |
8 |
9022082 |
+ |
ERI1 |
NNNCAAAAATAGTTTTCCAA |
NGG |
2 |
0.1238 |
Tier II |
| 9 |
NC_000012.12 |
12 |
93714133 |
+ |
CRADD |
NNNCTAAAATAGTTGTCCAA |
NGA |
2 |
0.0208 |
Tier II |
| 10 |
NC_000006.12 |
6 |
131844169 |
- |
ENPP1 |
NNNCCAAAATAGTTGTCCAA |
NGT |
2 |
0.0127 |
Tier II |
| 11 |
NC_000002.12 |
2 |
23130115 |
- |
LOC107985792 |
NNNAGAAAATAGATGTCCAA |
NGG |
2 |
0.583 |
Tier III |
| 12 |
NC_000004.12 |
4 |
23458777 |
- |
LOC105374524 |
NNNCCAAAATAGTTGTCCAA |
NGA |
2 |
0.0546 |
Tier III |
| 13 |
NC_000012.12 |
12 |
93714133 |
+ |
CRADD-AS1 |
NNNCTAAAATAGTTGTCCAA |
NGA |
2 |
0.0208 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
94619434 |
+ |
Gm32647 |
NNNCAAAAATACTTGTCCAA |
NGG |
2 |
0.4588 |
Tier III |
Other clones with same target sequence:
(none)
