Construct: sgRNA BRDN0001146099
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTACCCCAACAGCGACATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ATM (472)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77530
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
108250808 |
+ |
ATM |
NNNACCCCAACAGCGACATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
182634268 |
+ |
TENM3 |
NNNACTCAAACAGCGACATG |
NGG |
2 |
0.6036 |
Tier II |
| 3 |
NC_000002.12 |
2 |
120778589 |
- |
GLI2 |
NNNACCCCACCAGCCACATG |
NGG |
2 |
0.0909 |
Tier II |
| 4 |
NC_000015.10 |
15 |
25697821 |
+ |
ATP10A |
NNNACCCCAACAGTGACATG |
NTG |
2 |
0.0286 |
Tier II |
| 5 |
NC_000018.10 |
18 |
36716157 |
- |
FHOD3 |
NNNACCCCAACAGCCACATG |
NTG |
2 |
0.0106 |
Tier II |
| 6 |
NC_000013.11 |
13 |
74660493 |
+ |
LOC105370260 |
NNNACCCCAACAGAAACATG |
NGG |
2 |
0.3294 |
Tier III |
| 7 |
NC_000008.11 |
8 |
20917471 |
- |
LOC105379315 |
NNNACCCCAACAGAGACATG |
NGA |
2 |
0.0243 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
34490482 |
+ |
Kirrel3 |
NNNACCCCTACAGCAACATG |
NGG |
2 |
0.5647 |
Tier II |
| 2 |
NC_000083.6 |
17 |
44846244 |
- |
Supt3 |
NNNACCCCAACAGCAACAAG |
NGG |
2 |
0.2689 |
Tier II |
| 3 |
NC_000081.6 |
15 |
12223729 |
- |
Mtmr12 |
NNNAACCCAACAGCGAAATG |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000085.6 |
19 |
44194763 |
+ |
Pkd2l1 |
NNNACCCAAACAGCTACATG |
NGG |
2 |
0.0929 |
Tier II |
| 5 |
NC_000073.6 |
7 |
117490954 |
+ |
Xylt1 |
NNNACCCCAATAGCTACATG |
NGG |
2 |
0.044 |
Tier II |
| 6 |
NC_000078.6 |
12 |
24612835 |
- |
Grhl1 |
NNNACCCCAACAGGGACATG |
NGC |
2 |
0.0 |
Tier II |
| 7 |
NC_000077.6 |
11 |
5836066 |
- |
Polm |
NNNACCCCAACAGGGACTTG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000085.6 |
19 |
45811588 |
+ |
Kcnip2 |
NNNACCTCAACAGCGTCATG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000070.6 |
4 |
24329749 |
+ |
Gm27243 |
NNNACCCCATCAGAGACATG |
NGG |
2 |
0.3088 |
Tier III |
| 10 |
NC_000071.6 |
5 |
45959314 |
- |
Gm40298 |
NNNACCCCAACAGAGATATG |
NGG |
2 |
0.1633 |
Tier III |
Other clones with same target sequence:
(none)
