Construct: sgRNA BRDN0001146106

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTCCATAAAATCAAAGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK7 (1022)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76078

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 69255501 - CDK7 NNNCCATAAAATCAAAGACA NGG 0 1.0 Tier I
2 NC_000006.12 6 87426619 + CFAP206 NNNCCATAAAATCAAAGATA NCG 2 0.0495 Tier I
3 NC_000001.11 1 39347399 - MACF1 NNNACATAAAACCAAAGACA NGG 2 0.7978 Tier II
4 NC_000004.12 4 89034963 + FAM13A NNNTCATAATATCAAAGACA NGG 2 0.7059 Tier II
5 NC_000007.14 7 117030922 + ST7 NNNTCATAATATCAAAGACA NGG 2 0.7059 Tier II
6 NC_000012.12 12 20839599 - SLCO1B3 NNNACACAAAATCAAAGACA NGG 2 0.5789 Tier II
7 NC_000012.12 12 20839599 - SLCO1B3-SLCO1B7 NNNACACAAAATCAAAGACA NGG 2 0.5789 Tier II
8 NC_000007.14 7 2363619 - EIF3B NNNCCTTAAAAACAAAGACA NGG 2 0.5714 Tier II
9 NC_000005.10 5 77174277 - PDE8B NNNCCATAAAAACAGAGACA NGG 2 0.52 Tier II
10 NC_000012.12 12 133050929 - ZNF84 NNNCTATAAAAACAAAGACA NGG 2 0.5091 Tier II
11 NC_000009.12 9 105499605 - FSD1L NNNACAGAAAATCAAAGACA NGG 2 0.4954 Tier II
12 NC_000020.11 20 8647178 - PLCB1 NNNTGATAAAATCAAAGACA NGG 2 0.48 Tier II
13 NC_000010.11 10 107016667 + SORCS1 NNNCCATAAAAGCAAAAACA NGG 2 0.4667 Tier II
14 NC_000006.12 6 111195105 - SLC16A10 NNNCCCTAAAATCAAAGACA NGG 1 0.4545 Tier II
15 NC_000005.10 5 110582245 + TMEM232 NNNCCATAATAGCAAAGACA NGG 2 0.4412 Tier II
16 NC_000023.11 X 114664648 - HTR2C NNNCCATAATAGCAAAGACA NGG 2 0.4412 Tier II
17 NC_000002.12 2 217819285 + TNS1 NNNCCAAAAAAGCAAAGACA NGG 2 0.4375 Tier II
18 NC_000001.11 1 155503089 + ASH1L NNNCCGTAAAATCAAAGACT NGG 2 0.4286 Tier II
19 NC_000023.11 X 2431407 - DHRSX NNNTCATAAAAGCAAAGACA NGG 2 0.4 Tier II
20 NC_000024.10 Y 2431407 - DHRSX NNNTCATAAAAGCAAAGACA NGG 2 0.4 Tier II
21 NC_000006.12 6 143651426 + PHACTR2 NNNCCACAAAAGCAAAGACA NGG 2 0.3438 Tier II
22 NC_000013.11 13 48351009 - RB1 NNNCCACAAAAGCAAAGACA NGG 2 0.3438 Tier II
23 NC_000018.10 18 63347584 + KDSR NNNCCATCAAAACAAAGACA NGG 2 0.3429 Tier II
24 NC_000017.11 17 76393255 - UBE2O NNNCCAAAAAATAAAAGACA NGG 2 0.3365 Tier II
25 NC_000008.11 8 103561752 - RIMS2 NNNCCATAAAAACAAAGGCA NGG 2 0.32 Tier II
26 NC_000002.12 2 159927710 - PLA2R1 NNNCCATAAAATCAGAGAAA NGG 2 0.2786 Tier II
27 NC_000006.12 6 3406335 - SLC22A23 NNNCCATAATATCACAGACA NGG 2 0.2406 Tier II
28 NC_000015.10 15 32152864 + CHRNA7 NNNCCAGAAAATAAAAGACA NGG 2 0.2262 Tier II
29 NC_000002.12 2 190644088 + NEMP2 NNNCCATAAAAACAAATACA NGG 2 0.2 Tier II
30 NC_000003.12 3 160347620 + IFT80 NNNCCATACATTCAAAGACA NGG 2 0.1758 Tier II
31 NC_000021.9 21 45795398 - PCBP3 NNNCTATAAAATCAAATACA NGG 2 0.1591 Tier II
32 NC_000012.12 12 1128009 - ERC1 NNNCCACAAAATCAAAGCCA NGG 2 0.131 Tier II
33 NC_000005.10 5 160339220 + CCNJL NNNCAATAAAATCCAAGACA NGG 2 0.1224 Tier II
34 NC_000017.11 17 588613 - VPS53 NNNCCAAAAAATGAAAGACA NGG 2 0.1193 Tier II
35 NC_000005.10 5 120603105 - PRR16 NNNCCAAAAAATCAAAGAGA NGG 2 0.1094 Tier II
36 NC_000008.11 8 86498381 + RMDN1 NNNCCAAAAAATCAAAGAGA NGG 2 0.1094 Tier II
37 NC_000009.12 9 120633133 - MEGF9 NNNCCAAAAAATCAAAGAGA NGG 2 0.1094 Tier II
38 NC_000003.12 3 58869101 + CFAP20DC NNNCCATAAAATAAAACACA NGG 2 0.0905 Tier II
39 NC_000006.12 6 116529821 - CALHM4 NNNCCATAAAATAAAAGACC NGG 2 0.0874 Tier II
40 NC_000006.12 6 116529821 - TRAPPC3L NNNCCATAAAATAAAAGACC NGG 2 0.0874 Tier II
41 NC_000009.12 9 70225214 - MAMDC2 NNNCCATAACATCAAAGACA NAG 2 0.0864 Tier II
42 NC_000004.12 4 113550516 - CAMK2D NNNCGATAAAATCAAAGAGA NGG 2 0.075 Tier II
43 NC_000003.12 3 28242475 - CMC1 NNNCCATAAAATGAAAGAAA NGG 2 0.0584 Tier II
44 NC_000017.11 17 8908751 - PIK3R5 NNNCCATAAAAACAAAGACA NGA 2 0.0556 Tier II
45 NC_000009.12 9 124387573 + PSMB7 NNNCCATGAAATCAAAGACA NGA 2 0.0509 Tier II
46 NC_000021.9 21 34856699 - RUNX1 NNNTCATAAAATCAAAGACA NTG 2 0.0312 Tier II
47 NC_000010.11 10 96198256 + ZNF518A NNNCCATAGAATCAAAGACA NTG 2 0.026 Tier II
48 NC_000010.11 10 96198256 + BLNK NNNCCATAGAATCAAAGACA NTG 2 0.026 Tier II
49 NC_000014.9 14 70348258 + COX16 NNNCCATAATATCAAAGACA NGC 2 0.0196 Tier II
50 NC_000014.9 14 70348258 + SYNJ2BP-COX16 NNNCCATAATATCAAAGACA NGC 2 0.0196 Tier II
51 NC_000023.11 X 155561655 + TMLHE NNNCCATAAAATCAAAAACA NGT 2 0.0151 Tier II
52 NC_000008.11 8 41886206 - ANK1 NNNCCATAAAATCCAAGACA NGA 2 0.0149 Tier II
53 NC_000001.11 1 227637364 - ZNF678 NNNCCATAAGATCAAAGACA NGC 2 0.0123 Tier II
54 NC_000003.12 3 138321933 + NME9 NNNCCAGAAAATCAAAGACA NGT 2 0.0095 Tier II
55 NC_000005.10 5 167719127 + TENM2 NNNCCATAAAAGCAAAGACA NGT 2 0.0081 Tier II
56 NC_000001.11 1 183704786 - RGL1 NNNCCATAACATCAAAGACA NGC 2 0.0074 Tier II
57 NC_000001.11 1 246638978 - CNST NNNCCATAACATCAAAGACA NGC 2 0.0074 Tier II
58 NC_000002.12 2 142933072 - KYNU NNNCCATAACATCAAAGACA NGC 2 0.0074 Tier II
59 NC_000008.11 8 122832276 - ZHX2 NNNCCATAACATCAAAGACA NGC 2 0.0074 Tier II
60 NC_000014.9 14 75054701 + ACYP1 NNNCCATCAAATCAAAGACA NGT 2 0.0069 Tier II
61 NC_000004.12 4 53172307 - SCFD2 NNNCAATAAAATCAATGACA NGG 2 0.0 Tier II
62 NC_000006.12 6 122613641 + PKIB NNNCCATAAAATCAAAGACA NCT 2 0.0 Tier II
63 NC_000005.10 5 147426069 - DPYSL3 NNNCCATAAAATCAATGACC NGG 2 0.0 Tier II
64 NC_000012.12 12 54970666 + TESPA1 NNNCCATAAAATCAATGACC NGG 2 0.0 Tier II
65 NC_000002.12 2 2118529 + MYT1L NNNCCATAAAATCAATTACA NGG 2 0.0 Tier II
66 NC_000002.12 2 157211270 + CDK7P1 NNNCCATAAAACCAAAGACA NGG 1 0.9474 Tier III
67 NC_000005.10 5 93513399 + NR2F1-AS1 NNNCCACAATATCAAAGACA NGG 2 0.6066 Tier III
68 NC_000016.10 16 63605019 + LOC105371308 NNNTCACAAAATCAAAGACA NGG 2 0.55 Tier III
69 NC_000020.11 20 8647178 - LOC105372521 NNNTGATAAAATCAAAGACA NGG 2 0.48 Tier III
70 NC_000006.12 6 111195105 - LOC124901377 NNNCCCTAAAATCAAAGACA NGG 1 0.4545 Tier III
71 NC_000007.14 7 85435200 - LINC00972 NNNCCATAAAATCAAAAGCA NGG 2 0.3733 Tier III
72 NC_000005.10 5 125661887 - LOC124901056 NNNCCACAAAAGCAAAGACA NGG 2 0.3438 Tier III
73 NC_000001.11 1 160436840 + LOC105371467 NNNGAATAAAATCAAAGACA NGG 2 0.2857 Tier III
74 NC_000012.12 12 9723724 + CLECL1P NNNCCATAAAATCAAAAACA NAG 2 0.242 Tier III
75 NC_000003.12 3 160347620 + TRIM59-IFT80 NNNCCATACATTCAAAGACA NGG 2 0.1758 Tier III
76 NC_000012.12 12 1128009 - LOC124902857 NNNCCACAAAATCAAAGCCA NGG 2 0.131 Tier III
77 NC_000002.12 2 56150018 - LOC105374690 NNNCCATAAAATCAAAGAAA NAG 2 0.1111 Tier III
78 NC_000023.11 X 113216834 + LOC101928437 NNNCCATAAAATCAAAGGCC NGG 2 0.0909 Tier III
79 NC_000003.12 3 58869101 + CFAP20DC-AS1 NNNCCATAAAATAAAACACA NGG 2 0.0905 Tier III
80 NC_000009.12 9 70225214 - SMC5-DT NNNCCATAACATCAAAGACA NAG 2 0.0864 Tier III
81 NC_000002.12 2 188752029 - DIRC1 NNNCCATAAAATCACAGACA NAG 2 0.0707 Tier III
82 NC_000002.12 2 188752029 - LOC105373790 NNNCCATAAAATCACAGACA NAG 2 0.0707 Tier III
83 NC_000002.12 2 23365707 + LINC02923 NNNCCAAAAAATCAAAGACA NGA 2 0.0608 Tier III
84 NC_000015.10 15 98343956 - LINC02351 NNNCCATAAAATGAAACACA NGG 2 0.0321 Tier III
85 NC_000021.9 21 34856699 - RUNX1-AS1 NNNTCATAAAATCAAAGACA NTG 2 0.0312 Tier III
86 NC_000022.11 22 36851428 + NCF4-AS1 NNNCCATAAAATCATAGAGA NGG 2 0.025 Tier III
87 NC_000002.12 2 34079499 - LINC01317 NNNCCATAAAATCAAAGACT NTG 2 0.0234 Tier III
88 NC_000014.9 14 70348258 + LOC124903340 NNNCCATAATATCAAAGACA NGC 2 0.0196 Tier III
89 NC_000006.12 6 142540769 + LOC153910 NNNCCATAACATCAAAGACA NGC 2 0.0074 Tier III
90 NC_000011.10 11 93865907 - LOC101060084 NNNCCATAACATCAAAGACA NGC 2 0.0074 Tier III
91 NC_000019.10 19 20476408 - LOC105372316 NNNCCATAACATCAAAGACA NGC 2 0.0074 Tier III
92 NC_000023.11 X 78666314 - LOC107985670 NNNCCATAAAATCAAAGCCA NGT 2 0.0031 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 100719330 + Cdk7 NNNCCATAAAATCAAAGACA NGG 0 1.0 Tier I
2 NC_000082.6 16 10511886 + Ciita NNNCAATGAAATCAAAGACA NGG 2 0.419 Tier I
3 NC_000072.6 6 110759064 - Grm7 NNNACATAAAACCAAAGACA NGG 2 0.7978 Tier II
4 NC_000076.6 10 31847277 - Nkain2 NNNACATAAAACCAAAGACA NGG 2 0.7978 Tier II
5 NC_000070.6 4 100780075 - Cachd1 NNNACATAAAAACAAAGACA NGG 2 0.6737 Tier II
6 NC_000070.6 4 65290497 - Pappa NNNCCACAAAACCAAAGACA NGG 2 0.6513 Tier II
7 NC_000072.6 6 22993289 - Ptprz1 NNNCTATAAAATCAAAAACA NGG 2 0.5939 Tier II
8 NC_000069.6 3 57757689 - Rnf13 NNNACATAAAATCAGAGACA NGG 2 0.5474 Tier II
9 NC_000067.6 1 88376805 + Trpm8 NNNCTATAAAAACAAAGACA NGG 2 0.5091 Tier II
10 NC_000069.6 3 25427492 - Nlgn1 NNNCCATATAAACAAAGACA NGG 2 0.48 Tier II
11 NC_000074.6 8 79982076 + Hhip NNNCAATAAAAACAAAGACA NGG 2 0.4571 Tier II
12 NC_000086.7 X 83137780 - Dmd NNNCCACAAAATCAGAGACA NGG 2 0.4469 Tier II
13 NC_000067.6 1 182798948 - Susd4 NNNCCATGAAATCAAAGACT NGG 2 0.44 Tier II
14 NC_000085.6 19 10431379 + Syt7 NNNCCATGAGATCAAAGACA NGG 2 0.4074 Tier II
15 NC_000071.6 5 27344047 - Dpp6 NNNCCTTAAGATCAAAGACA NGG 2 0.3968 Tier II
16 NC_000069.6 3 100862042 + Vtcn1 NNNCCATAGGATCAAAGACA NGG 2 0.3704 Tier II
17 NC_000070.6 4 57080433 + Epb41l4b NNNCAATAAAATCAAAGTCA NGG 2 0.2857 Tier II
18 NC_000067.6 1 62236510 - Pard3b NNNTCATAAATTCAAAGACA NGG 2 0.2462 Tier II
19 NC_000084.6 18 23439607 - Dtna NNNCCATAAAACCAAAGACA NAG 2 0.2456 Tier II
20 NC_000083.6 17 45070492 - Supt3 NNNCCATTAAATCTAAGACA NGG 2 0.2286 Tier II
21 NC_000070.6 4 65569021 - Astn2 NNNACATAAAATCAAAGACA NAG 2 0.2183 Tier II
22 NC_000086.7 X 142098851 + Gucy2f NNNTCATAAAATCAAACACA NGG 2 0.1882 Tier II
23 NC_000075.6 9 95228384 + Slc9a9 NNNCCATTAAATCAAAGAAA NGG 2 0.1837 Tier II
24 NC_000069.6 3 84906394 + Fbxw7 NNNCCATAAAAACAAAGACC NGG 2 0.1818 Tier II
25 NC_000069.6 3 95396437 + 4930558C23Rik NNNCCATAAAATAAAAGATA NGG 2 0.1775 Tier II
26 NC_000075.6 9 21982988 - Rgl3 NNNCCATCAAATCAAAGGCA NGG 2 0.1714 Tier II
27 NC_000086.7 X 49272280 + Enox2 NNNCCATAAATTCAAAGAAA NGG 2 0.1319 Tier II
28 NC_000082.6 16 74451265 - Robo2 NNNCCATAAAAGCAAAGACA NAG 2 0.1296 Tier II
29 NC_000075.6 9 50753886 - Cryab NNNCCAGAAAATCATAGACA NGG 2 0.1176 Tier II
30 NC_000072.6 6 70855054 + Eif2ak3 NNNCCATAAAATCAAACTCA NGG 2 0.1176 Tier II
31 NC_000075.6 9 62109600 - Glce NNNCCATAAAAAGAAAGACA NGG 2 0.1091 Tier II
32 NC_000067.6 1 161822132 - Suco NNNCCATAACATCCAAGACA NGG 2 0.0714 Tier II
33 NC_000068.7 2 43802873 - Arhgap15 NNNCCATAAAAGGAAAGACA NGG 2 0.0682 Tier II
34 NC_000079.6 13 50240983 - Gm906 NNNCCATAAAATAAAAGAGA NGG 2 0.0481 Tier II
35 NC_000082.6 16 13267600 - Mrtfb NNNCCATAAAATCAAATCCA NGG 2 0.0476 Tier II
36 NC_000068.7 2 19227148 - Armc3 NNNACATAAAATCAAAGACA NTG 2 0.0328 Tier II
37 NC_000069.6 3 29226082 + Egfem1 NNNACATAAAATCAAAGACA NTG 2 0.0328 Tier II
38 NC_000069.6 3 118971051 - Dpyd NNNACATAAAATCAAAGACA NTG 2 0.0328 Tier II
39 NC_000070.6 4 19833097 - Slc7a13 NNNACATAAAATCAAAGACA NTG 2 0.0328 Tier II
40 NC_000084.6 18 37911601 + Diaph1 NNNCCATAAAATCAAAGAAA NGA 2 0.0298 Tier II
41 NC_000071.6 5 45741864 + Lcorl NNNCCATAAAATCAAACACA NGA 2 0.0163 Tier II
42 NC_000083.6 17 21537967 - Zfp52 NNNCCATAAAATAAAAGACA NTG 2 0.015 Tier II
43 NC_000074.6 8 40794060 + Adam20 NNNCCATGAAATCAAAGACA NGT 2 0.0118 Tier II
44 NC_000076.6 10 127766630 - Rdh1 NNNCCATAAAATGAAAGACA NGA 2 0.0095 Tier II
45 NC_000082.6 16 23122876 - Rfc4 NNNCCATAAAATCAAAGCCA NGT 2 0.0031 Tier II
46 NC_000073.6 7 94243861 - Gm32647 NNNACATAAAATCAGAGACA NGG 2 0.5474 Tier III
47 NC_000073.6 7 94524033 - Gm32647 NNNACATAAAATCAGAGACA NGG 2 0.5474 Tier III
48 NC_000082.6 16 10511886 + LOC115488669 NNNCAATGAAATCAAAGACA NGG 2 0.419 Tier III
49 NC_000076.6 10 97716955 - 4930525C09Rik NNNCCGTAAAATCAAAGAAA NGG 2 0.3061 Tier III
50 NC_000076.6 10 97716955 - Gm10754 NNNCCGTAAAATCAAAGAAA NGG 2 0.3061 Tier III
51 NC_000084.6 18 57423499 + Gm34982 NNNCCATCAAATCAAAGATA NGG 2 0.1978 Tier III
52 NC_000069.6 3 17365836 + Gm30340 NNNCTATAAAATCAAGGACA NGG 2 0.1224 Tier III
53 NC_000067.6 1 105923554 + Gm41927 NNNCCAGAAAATCAAAGAGA NGG 2 0.0735 Tier III
54 NC_000067.6 1 161822132 - Gm7496 NNNCCATAACATCCAAGACA NGG 2 0.0714 Tier III
55 NC_000068.7 2 78802273 - Gm39865 NNNGCATAAAATGAAAGACA NGG 2 0.0682 Tier III
56 NC_000075.6 9 103936857 - Gm29154 NNNCCATGAAATCAAAGACA NTG 2 0.0286 Tier III
57 NC_000069.6 3 35606048 - Gm6639 NNNCCATAAAATCAGAGACA NTG 2 0.0253 Tier III
58 NC_000074.6 8 33731333 + 1700104B16Rik NNNCCAAAAAATCAAAGACA NGT 2 0.0141 Tier III
Download CSV

Other clones with same target sequence:

(none)