Construct: sgRNA BRDN0001146108
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCACCTGGACACATTCCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKM (5213)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77078
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
48137831 |
- |
PFKM |
NNNCCTGGACACATTCCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
38412722 |
- |
RASGRP4 |
NNNCCTGCACACCTTCCATG |
NGG |
2 |
0.1296 |
Tier I |
| 3 |
NC_000011.10 |
11 |
65530693 |
+ |
SCYL1 |
NNNCCTGGACGCATTCCCTG |
NGG |
2 |
0.1238 |
Tier I |
| 4 |
NC_000007.14 |
7 |
16662349 |
- |
BZW2 |
NNNCCTCGATACATTCCATG |
NGG |
2 |
0.6471 |
Tier II |
| 5 |
NC_000014.9 |
14 |
72748803 |
+ |
DPF3 |
NNNCCTTGACAAATTCCATG |
NGG |
2 |
0.4082 |
Tier II |
| 6 |
NC_000005.10 |
5 |
109756200 |
- |
MAN2A1 |
NNNACTGGACCCATTCCATG |
NGG |
2 |
0.3368 |
Tier II |
| 7 |
NC_000001.11 |
1 |
178036856 |
+ |
CRYZL2P-SEC16B |
NNNCCTGGACACATTCCATG |
NAG |
1 |
0.2593 |
Tier II |
| 8 |
NC_000020.11 |
20 |
4863617 |
+ |
SLC23A2 |
NNNCCTGCACCCATTCCATG |
NGG |
2 |
0.2462 |
Tier II |
| 9 |
NC_000003.12 |
3 |
10673330 |
- |
ATP2B2 |
NNNCCTGGACACACTCCATT |
NGG |
2 |
0.2 |
Tier II |
| 10 |
NC_000010.11 |
10 |
70380815 |
+ |
LRRC20 |
NNNCCTGGACACCTTCCATA |
NGG |
2 |
0.1974 |
Tier II |
| 11 |
NC_000017.11 |
17 |
6446982 |
+ |
PIMREG |
NNNCGTGGACTCATTCCATG |
NGG |
2 |
0.1846 |
Tier II |
| 12 |
NC_000020.11 |
20 |
61763526 |
- |
CDH4 |
NNNTCTGGACACATTGCATG |
NGG |
2 |
0.1231 |
Tier II |
| 13 |
NC_000011.10 |
11 |
822053 |
+ |
PNPLA2 |
NNNCCTGGACACCTTCTATG |
NGG |
2 |
0.0982 |
Tier II |
| 14 |
NC_000020.11 |
20 |
20298812 |
- |
CFAP61 |
NNNCCTGAACACATGCCATG |
NGG |
2 |
0.05 |
Tier II |
| 15 |
NC_000001.11 |
1 |
6329608 |
+ |
ACOT7 |
NNNCCTGGACACAAGCCATG |
NGG |
2 |
0.031 |
Tier II |
| 16 |
NC_000001.11 |
1 |
230344687 |
+ |
PGBD5 |
NNNCCTGGACAGATTCCATG |
NTG |
2 |
0.0173 |
Tier II |
| 17 |
NC_000016.10 |
16 |
73158229 |
+ |
ZFHX3 |
NNNCCTGGACACATACCATG |
NGT |
2 |
0.0093 |
Tier II |
| 18 |
NC_000014.9 |
14 |
64096423 |
- |
SYNE2 |
NNNCCTGGCCACAGTCCATG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000014.9 |
14 |
49085484 |
- |
LOC105378178 |
NNNCCTTAACACATTCCATG |
NGG |
2 |
0.5714 |
Tier III |
| 20 |
NC_000004.12 |
4 |
34669728 |
- |
LOC107986271 |
NNNCTTGCACACATTCCATG |
NGG |
2 |
0.3916 |
Tier III |
| 21 |
NC_000014.9 |
14 |
106282347 |
- |
IGH |
NNNGCTGGGCACATTCCATG |
NGG |
2 |
0.3333 |
Tier III |
| 22 |
NC_000015.10 |
15 |
100865261 |
- |
GCAWKR |
NNNCATGGCCACATTCCATG |
NGG |
2 |
0.3265 |
Tier III |
| 23 |
NC_000001.11 |
1 |
178036856 |
+ |
CRYZL2P |
NNNCCTGGACACATTCCATG |
NAG |
1 |
0.2593 |
Tier III |
| 24 |
NC_000017.11 |
17 |
6446982 |
+ |
LOC107985017 |
NNNCGTGGACTCATTCCATG |
NGG |
2 |
0.1846 |
Tier III |
| 25 |
NC_000013.11 |
13 |
76727987 |
+ |
LOC105370265 |
NNNCCTTGACACCTTCCATG |
NGG |
2 |
0.1203 |
Tier III |
| 26 |
NC_000002.12 |
2 |
12503936 |
+ |
MIR3681HG |
NNNCCTGCACACATTCCATG |
NGC |
2 |
0.0137 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
40279514 |
- |
Scn3b |
NNNCCTGGACACATTACATG |
NGT |
2 |
0.0161 |
Tier I |
| 2 |
NC_000078.6 |
12 |
37922504 |
- |
Dgkb |
NNNCCTGACCACATTCCATG |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000072.6 |
6 |
147107627 |
- |
Klhl42 |
NNNCCTGCACACATACCATG |
NGG |
2 |
0.3563 |
Tier II |
| 4 |
NC_000072.6 |
6 |
128422170 |
+ |
Itfg2 |
NNNCCTGGACAGAATCCATG |
NGG |
2 |
0.2751 |
Tier II |
| 5 |
NC_000078.6 |
12 |
32190985 |
- |
Pik3cg |
NNNACTGGACACATTTCATG |
NGG |
2 |
0.2591 |
Tier II |
| 6 |
NC_000077.6 |
11 |
36012522 |
+ |
Tenm2 |
NNNCCTGGACTCATTCCTTG |
NGG |
2 |
0.1538 |
Tier II |
| 7 |
NC_000082.6 |
16 |
26077921 |
- |
P3h2 |
NNNGCTGGACACTTTCCATG |
NGG |
2 |
0.15 |
Tier II |
| 8 |
NC_000083.6 |
17 |
31579983 |
- |
Pknox1 |
NNNGCTGGACACTTTCCATG |
NGG |
2 |
0.15 |
Tier II |
| 9 |
NC_000067.6 |
1 |
54046246 |
- |
Hecw2 |
NNNCCTTGACACCTTCCATG |
NGG |
2 |
0.1203 |
Tier II |
| 10 |
NC_000082.6 |
16 |
72158044 |
+ |
Robo1 |
NNNCCTGGACACTTTCCACG |
NGG |
2 |
0.0828 |
Tier II |
| 11 |
NC_000072.6 |
6 |
72517317 |
+ |
Sh2d6 |
NNNCCTGGAGACCTTCCATG |
NGG |
2 |
0.0819 |
Tier II |
| 12 |
NC_000081.6 |
15 |
76465059 |
+ |
Bop1 |
NNNCCTGGACACATTCCCTG |
NAG |
2 |
0.0494 |
Tier II |
| 13 |
NC_000081.6 |
15 |
53689497 |
+ |
Samd12 |
NNNCCTGGACACATTCCATG |
NGC |
1 |
0.0222 |
Tier II |
| 14 |
NC_000068.7 |
2 |
21743700 |
+ |
Gpr158 |
NNNCCTGGACACATTCCTTG |
NGC |
2 |
0.0111 |
Tier II |
| 15 |
NC_000072.6 |
6 |
28688412 |
+ |
Snd1 |
NNNGCTGGACACATTCCATG |
NGC |
2 |
0.0111 |
Tier II |
| 16 |
NC_000071.6 |
5 |
117878442 |
- |
Nos1 |
NNNCCTGGACACAGTCCATG |
NGG |
1 |
0.0 |
Tier II |
| 17 |
NC_000082.6 |
16 |
4712378 |
- |
Nmral1 |
NNNCCTGGACACATTCCATG |
NCC |
2 |
0.0 |
Tier II |
| 18 |
NC_000077.6 |
11 |
36012522 |
+ |
Gm42072 |
NNNCCTGGACTCATTCCTTG |
NGG |
2 |
0.1538 |
Tier III |
| 19 |
NC_000071.6 |
5 |
117878442 |
- |
Gm30360 |
NNNCCTGGACACAGTCCATG |
NGG |
1 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
