Construct: sgRNA BRDN0001146109

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GACAGTGAAATTAGCTCCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80242

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 65393179 + MAGI1 NNNAGTGAATTCAGCTCCCA NGG 2 0.8359 Tier II
2 NC_000002.12 2 233794099 - MROH2A NNNTGTGAAAATAGCTCCCA NGG 2 0.4773 Tier II
3 NC_000005.10 5 68283937 + PIK3R1 NNNTGTGAAATTAGCCCCCA NGG 2 0.4242 Tier II
4 NC_000007.14 7 47285847 + TNS3 NNNGGTGAAATTAGCTCCCT NGG 2 0.375 Tier II
5 NC_000012.12 12 40013700 - SLC2A13 NNNAGTGAAATAAGCTCCAA NGG 2 0.3429 Tier II
6 NC_000023.11 X 139922605 - ATP11C NNNAGTGAAATGAGCTCCCT NGG 2 0.3 Tier II
7 NC_000012.12 12 70352588 - CNOT2 NNNAGTGACATTAGCTACCA NGG 2 0.2667 Tier II
8 NC_000003.12 3 59993696 - FHIT NNNAGTGAAATCAGCTCCCA NAG 2 0.2456 Tier II
9 NC_000008.11 8 143475189 + ZC3H3 NNNAGTGAAATTAACTCCCA NGA 2 0.0521 Tier II
10 NC_000007.14 7 2694037 - AMZ1 NNNAGTGACATTAGCTGCCA NGG 2 0.0336 Tier II
11 NC_000001.11 1 67398298 - IL12RB2 NNNAGTGAAGTTAGCTCCCA NTG 2 0.0216 Tier II
12 NC_000023.11 X 19690933 - SH3KBP1 NNNAGTGAAATTAACTCCCA NGC 2 0.0167 Tier II
13 NC_000017.11 17 43148008 - BRCA1 NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier II
14 NC_000017.11 17 46335909 + LRRC37A NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier II
15 NC_000017.11 17 46335909 + ARL17B NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier II
16 NC_000017.11 17 46553764 + ARL17A NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier II
17 NC_000017.11 17 46553764 + LRRC37A2 NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier II
18 NC_000017.11 17 64855520 - LRRC37A3 NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier II
19 NC_000004.12 4 75997360 - SDAD1-AS1 NNNAGTGGAATTAGCTCCCA NGT 2 0.0118 Tier III
20 NC_000017.11 17 43148008 - NBR2 NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier III
21 NC_000017.11 17 45509922 - LRRC37A4P NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier III
22 NC_000017.11 17 45509922 - LOC105369225 NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier III
23 NC_000017.11 17 64855520 - LOC105376844 NNNAGTGAAATTAGCTCCCC NGC 2 0.0051 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 20130942 - Slc9a7 NNNAGAGAAAATAGCTCCCA NGG 2 0.65 Tier II
2 NC_000079.6 13 95860211 + Iqgap2 NNNACTGAAAATAGCTCCCA NGG 2 0.5893 Tier II
3 NC_000068.7 2 121567740 + Frmd5 NNNAGTGAAATCACCTCCCA NGG 2 0.406 Tier II
4 NC_000074.6 8 84371239 - Cacna1a NNNAGTGAAAGTAGCTCCCA NGG 1 0.4 Tier II
5 NC_000072.6 6 38983389 + Tbxas1 NNNAGGGAAATTAGCCCCCA NGG 2 0.381 Tier II
6 NC_000067.6 1 188176194 - Esrrg NNNAGAGAAAGTAGCTCCCA NGG 2 0.3467 Tier II
7 NC_000067.6 1 123573811 - Dpp10 NNNAGTTAAATGAGCTCCCA NGG 2 0.2857 Tier II
8 NC_000080.6 14 28121448 + Erc2 NNNAGTGAAATCTGCTCCCA NGG 2 0.2842 Tier II
9 NC_000084.6 18 45679763 - Kcnn2 NNNAGTGAAAATTGCTCCCA NGG 2 0.225 Tier II
10 NC_000073.6 7 89326610 + Tmem135 NNNAGTGAACTTAGCTCCCC NGG 2 0.0758 Tier II
11 NC_000077.6 11 20562376 - Sertad2 NNNAGTGAAATTAGCTCCCA NGA 1 0.0694 Tier II
12 NC_000072.6 6 3727885 - Calcr NNNATTGAAATTAGCTCCCC NGG 2 0.0682 Tier II
13 NC_000075.6 9 114789477 - Cmtm8 NNNAGTTAAATTAGCTCCCA NTG 2 0.0223 Tier II
14 NC_000082.6 16 94247787 - Hlcs NNNAGTGACATTAGCTCCCA NGT 2 0.0092 Tier II
15 NC_000080.6 14 52584168 + Tcra NNNAGTGAAAATAGCACCCA NGG 2 0.6818 Tier III
16 NC_000080.6 14 44891103 - Gm16976 NNNAGTGAAATTATTTCCCA NGG 2 0.0178 Tier III
Download CSV

Other clones with same target sequence:

(none)

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