Construct: sgRNA BRDN0001146110
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATAACCAGTCTTGTCACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FER (2241)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76802
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
108871409 |
+ |
FER |
NNNAACCAGTCTTGTCACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
42414351 |
- |
TRERF1 |
NNNAACCAGGCTTGTAACAG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000010.11 |
10 |
80142059 |
- |
PLAC9 |
NNNAACAAGTCTTGTCACTG |
NGG |
2 |
0.4038 |
Tier II |
| 4 |
NC_000002.12 |
2 |
46089133 |
+ |
PRKCE |
NNNAACCATTCTTATCACAG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000023.11 |
X |
148726393 |
+ |
AFF2 |
NNNAAACAGTCTTGTCACGG |
NGG |
2 |
0.3482 |
Tier II |
| 6 |
NC_000007.14 |
7 |
106903408 |
+ |
PIK3CG |
NNNAAGCAGTCTTGTCACTG |
NGG |
2 |
0.2692 |
Tier II |
| 7 |
NC_000011.10 |
11 |
33108894 |
- |
CSTF3 |
NNNGACCTGTCTTGTCACAG |
NGG |
2 |
0.2679 |
Tier II |
| 8 |
NC_000012.12 |
12 |
40879734 |
- |
CNTN1 |
NNNAACCTGTCTTCTCACAG |
NGG |
2 |
0.1837 |
Tier II |
| 9 |
NC_000002.12 |
2 |
144450969 |
- |
ZEB2 |
NNNAACCAGGCTGGTCACAG |
NGG |
2 |
0.1304 |
Tier II |
| 10 |
NC_000002.12 |
2 |
153245049 |
- |
GALNT13 |
NNNAAGCAGTCTGGTCACAG |
NGG |
2 |
0.1304 |
Tier II |
| 11 |
NC_000022.11 |
22 |
41752541 |
- |
MEI1 |
NNNAACCAGACTTGTCACAG |
NGA |
2 |
0.0595 |
Tier II |
| 12 |
NC_000003.12 |
3 |
50114894 |
+ |
RBM5 |
NNNAAACAGTCTTGGCACAG |
NGG |
2 |
0.0464 |
Tier II |
| 13 |
NC_000015.10 |
15 |
45590699 |
- |
BLOC1S6 |
NNNAAACAGTCTTGGCACAG |
NGG |
2 |
0.0464 |
Tier II |
| 14 |
NC_000007.14 |
7 |
132906571 |
- |
CHCHD3 |
NNNAACCAGTCTTGTCACAC |
NGC |
2 |
0.0095 |
Tier II |
| 15 |
NC_000011.10 |
11 |
82259814 |
- |
MIR4300HG |
NNNAACTAGGCTTGTCACAG |
NGG |
2 |
0.4063 |
Tier III |
| 16 |
NC_000002.12 |
2 |
129925811 |
+ |
PLAC9P1 |
NNNAACAAGTCTTGTCACTG |
NGG |
2 |
0.4038 |
Tier III |
| 17 |
NC_000002.12 |
2 |
129925811 |
+ |
LINC01856 |
NNNAACAAGTCTTGTCACTG |
NGG |
2 |
0.4038 |
Tier III |
| 18 |
NC_000004.12 |
4 |
116883151 |
+ |
LOC107986306 |
NNNAACCAGTCTGGCCACAG |
NGG |
2 |
0.0711 |
Tier III |
| 19 |
NC_000002.12 |
2 |
207223562 |
+ |
MYOSLID-AS1 |
NNNAACCAGGCTTGTCAGAG |
NGG |
2 |
0.0667 |
Tier III |
| 20 |
NC_000003.12 |
3 |
177680644 |
- |
LINC00578 |
NNNAACCAGTCTAGTCACAG |
NGT |
2 |
0.0112 |
Tier III |
| 21 |
NC_000017.11 |
17 |
64951004 |
- |
LOC107985000 |
NNNTACCAGTCTTGTCACAG |
NGT |
2 |
0.0103 |
Tier III |
| 22 |
NC_000017.11 |
17 |
68190084 |
+ |
LOC105371870 |
NNNTACCAGTCTTGTCACAG |
NGT |
2 |
0.0103 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
110658338 |
+ |
Dync1h1 |
NNNAATCTGTCTTGTCACAG |
NGG |
2 |
0.398 |
Tier I |
| 2 |
NC_000083.6 |
17 |
63923406 |
+ |
Fer |
NNNAACCAGCCTTGTTACAG |
NGG |
2 |
0.1641 |
Tier I |
| 3 |
NC_000078.6 |
12 |
26331547 |
+ |
Rnf144a |
NNNAACCAGTCTTGTCACAG |
NGG |
0 |
1.0 |
Tier II |
| 4 |
NC_000076.6 |
10 |
86813437 |
- |
Nt5dc3 |
NNNAACCAGTCTAGTCACAG |
NGG |
1 |
0.6923 |
Tier II |
| 5 |
NC_000068.7 |
2 |
37555881 |
- |
Rabgap1 |
NNNAACCAGTCTTGAAACAG |
NGG |
2 |
0.5789 |
Tier II |
| 6 |
NC_000071.6 |
5 |
124506733 |
+ |
Rilpl1 |
NNNAAACATTCTTGTCACAG |
NGG |
2 |
0.4952 |
Tier II |
| 7 |
NC_000068.7 |
2 |
22807292 |
- |
Apbb1ip |
NNNAACCACACTTGTCACAG |
NGG |
2 |
0.4615 |
Tier II |
| 8 |
NC_000074.6 |
8 |
40345194 |
+ |
Micu3 |
NNNAACCAGTATTGTCACTG |
NGG |
2 |
0.4038 |
Tier II |
| 9 |
NC_000069.6 |
3 |
96873226 |
- |
Gpr89 |
NNNAACCAAGCTTGTCACAG |
NGG |
2 |
0.3214 |
Tier II |
| 10 |
NC_000069.6 |
3 |
153688858 |
- |
St6galnac3 |
NNNGAGCAGTCTTGTCACAG |
NGG |
2 |
0.3125 |
Tier II |
| 11 |
NC_000068.7 |
2 |
29224828 |
+ |
Ntng2 |
NNNCCCCAGTCTTGTCACAG |
NGG |
2 |
0.1765 |
Tier II |
| 12 |
NC_000084.6 |
18 |
9383581 |
- |
Ccny |
NNNAACCAATCTTTTCACAG |
NGG |
2 |
0.1714 |
Tier II |
| 13 |
NC_000067.6 |
1 |
174244956 |
+ |
Spta1 |
NNNAACCATTCTTGTCACAG |
NAG |
2 |
0.1383 |
Tier II |
| 14 |
NC_000079.6 |
13 |
49767548 |
- |
Gm272 |
NNNAACCAGTCTTCTCCCAG |
NGG |
2 |
0.0756 |
Tier II |
| 15 |
NC_000074.6 |
8 |
110696050 |
+ |
Vac14 |
NNNAACCTGTCTTGTCACAG |
NCG |
2 |
0.0459 |
Tier II |
| 16 |
NC_000073.6 |
7 |
47596779 |
- |
Mrgpra3 |
NNNAAGCAGTCTTGTCACAG |
NGA |
2 |
0.0347 |
Tier II |
| 17 |
NC_000075.6 |
9 |
29716726 |
- |
Ntm |
NNNAACCTGTCTTGTCACAG |
NGA |
2 |
0.0298 |
Tier II |
| 18 |
NC_000070.6 |
4 |
140558675 |
+ |
Arhgef10l |
NNNATCCAGTCTTGTCACAG |
NTG |
2 |
0.0142 |
Tier II |
| 19 |
NC_000077.6 |
11 |
55566968 |
- |
Glra1 |
NNNAACCAGTCTTCTCACAG |
NGC |
2 |
0.0095 |
Tier II |
| 20 |
NC_000082.6 |
16 |
25441930 |
- |
Gm18896 |
NNNAACCAGTCTAGTTACAG |
NGG |
2 |
0.213 |
Tier III |
| 21 |
NC_000073.6 |
7 |
34837908 |
- |
Gm38987 |
NNNAACCAGTCTTTTTACAG |
NGG |
2 |
0.0821 |
Tier III |
Other clones with same target sequence:
(none)
