Construct: sgRNA BRDN0001146112

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAAAAGCTTTCTGCTAGGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2D (817)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77185

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 113759322 - CAMK2D NNNAAGCTTTCTGCTAGGGG NGG 0 1.0 Tier I
2 NC_000008.11 8 40642666 + ZMAT4 NNNAAGCTATCTGCTAGAGG NGG 2 0.6429 Tier II
3 NC_000009.12 9 69861528 + CFAP95 NNNTAGCTTTCTGCTAAGGG NGG 2 0.5939 Tier II
4 NC_000002.12 2 121523501 - CLASP1 NNNAAGTTTTCTGCTAGAGG NGG 2 0.5625 Tier II
5 NC_000009.12 9 75936191 + PCSK5 NNNAATCTTTCTGCTAGTGG NGG 2 0.4444 Tier II
6 NC_000014.9 14 70067988 + SLC8A3 NNNAAGCTTTCTGCCAAGGG NGG 2 0.2545 Tier II
7 NC_000004.12 4 108123427 + LEF1 NNNCAGCTTTCTGCTAGGGT NGG 2 0.2471 Tier II
8 NC_000002.12 2 112985357 + IL36G NNNGAGCTTTCTTCTAGGGG NGG 2 0.1875 Tier II
9 NC_000007.14 7 99247469 - MYH16 NNNAAGCTTTCTGCCAGGTG NGG 2 0.1818 Tier II
10 NC_000008.11 8 143559188 + GSDMD NNNAAGCTTTCTGCTAGTGG NAG 2 0.1728 Tier II
11 NC_000001.11 1 35289050 + ZMYM4 NNNAAGCTTTGTGCTAGTGG NGG 2 0.1667 Tier II
12 NC_000008.11 8 29232881 + KIF13B NNNAAGCTTTCAGCTGGGGG NGG 2 0.1538 Tier II
13 NC_000011.10 11 86409816 + CCDC81 NNNAAGCTTTCTTCTAGGGC NGG 2 0.1286 Tier II
14 NC_000011.10 11 17349074 - NUCB2 NNNAAGCTTTCTGCTGGGCG NGG 2 0.0862 Tier II
15 NC_000012.12 12 57427667 + R3HDM2 NNNAAGCTTTCTACTAGGGG NGA 2 0.0641 Tier II
16 NC_000001.11 1 100048121 + MFSD14A NNNAAGCTTTCTGCTAGGGG NTG 1 0.039 Tier II
17 NC_000016.10 16 13420374 - SHISA9 NNNAAGCTTTCTGTGAGGGG NGG 2 0.0367 Tier II
18 NC_000010.11 10 19200965 - MALRD1 NNNAAGCTTTGTGCTAGGGG NTG 2 0.0097 Tier II
19 NC_000005.10 5 180553551 + CNOT6 NNNAAGCTTTCTGCTAGGGG NTT 2 0.0 Tier II
20 NC_000011.10 11 72357003 + CLPB NNNAAGCTTTCTGCTTGGGG NTG 2 0.0 Tier II
21 NC_000007.14 7 44285467 - CAMK2B NNNAAGCTTTCTGGGAGGGG NGG 2 0.0 Tier II
22 NC_000008.11 8 40642666 + LOC107986938 NNNAAGCTATCTGCTAGAGG NGG 2 0.6429 Tier III
23 NC_000001.11 1 38089065 - MIR3659HG NNNGAGCTGTCTGCTAGGGG NGG 2 0.3869 Tier III
24 NC_000006.12 6 134805005 + LOC101928277 NNNAAGCCTTCTGCTGGGGG NGG 2 0.1923 Tier III
25 NC_000006.12 6 81930087 - LINC02542 NNNAAGCTTACTGCTGGGGG NGG 2 0.1648 Tier III
26 NC_000010.11 10 53013398 - LINC02672 NNNAAGGTTTCTGCTATGGG NGG 2 0.1176 Tier III
27 NC_000008.11 8 123008381 + LOC124902013 NNNAAGCTTTCTGCTAGGTG NTG 2 0.026 Tier III
28 NC_000011.10 11 72357003 + LOC112268077 NNNAAGCTTTCTGCTTGGGG NTG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 126599159 + Camk2d NNNAAGCTTTCTGCTAGGGG NGG 0 1.0 Tier I
2 NC_000080.6 14 24135574 + Dlg5 NNNGAGCTTTCTGCTAGGGT NGG 2 0.4375 Tier I
3 NC_000077.6 11 67598745 - Gas7 NNNAAGCTTCCTGCTAGGGG NGG 1 0.5333 Tier II
4 NC_000083.6 17 47151829 + Trerf1 NNNAAGCTCTCGGCTAGGGG NGG 2 0.4615 Tier II
5 NC_000083.6 17 46506923 - Cul9 NNNAGGCTGTCTGCTAGGGG NGG 2 0.4457 Tier II
6 NC_000077.6 11 95267008 - Tac4 NNNAAGCTGTCTGCTAGGGT NGG 2 0.4333 Tier II
7 NC_000076.6 10 80261447 + Dazap1 NNNCAGCCTTCTGCTAGGGG NGG 2 0.3529 Tier II
8 NC_000073.6 7 18482084 - Psg26 NNNAAGCTTTGTGCTAGGGG NGG 1 0.25 Tier II
9 NC_000067.6 1 87478809 + Ngef NNNAAGCTTCCTCCTAGGGG NGG 2 0.2246 Tier II
10 NC_000082.6 16 34383466 - Kalrn NNNAAGATTTCTGCTGGGGG NGG 2 0.1442 Tier II
11 NC_000072.6 6 23500278 + Cadps2 NNNAAGGTTTGTGCTAGGGG NGG 2 0.1176 Tier II
12 NC_000073.6 7 18352900 - Psg18 NNNAAGCTTTGTGCTAGGGC NGG 2 0.1071 Tier II
13 NC_000073.6 7 18429966 - Psg28 NNNAAGCTTTGTGCTAGGGC NGG 2 0.1071 Tier II
14 NC_000075.6 9 58107939 + Stra6 NNNAAGCTTTCTTCTGGGGG NGG 2 0.0577 Tier II
15 NC_000075.6 9 58107939 + Ccdc33 NNNAAGCTTTCTTCTGGGGG NGG 2 0.0577 Tier II
16 NC_000083.6 17 67142724 - Ptprm NNNAAGCTTTCTGCTGTGGG NGG 2 0.0481 Tier II
17 NC_000068.7 2 6799966 - Celf2 NNNAAGCCTTCTGCTAGGGG NTG 2 0.039 Tier II
18 NC_000083.6 17 24857331 - Hagh NNNATGCTTTCTGCTAGGGG NTG 2 0.0142 Tier II
19 NC_000070.6 4 132649415 - Eya3 NNNAAGCTTTCTGCTGGGGG NTG 2 0.0075 Tier II
20 NC_000070.6 4 119851869 + Hivep3 NNNAAGATTTCTGCTTGGGG NGG 2 0.0 Tier II
21 NC_000071.6 5 128064142 - Tmem132d NNNAAGCTTTCTGCTTGGGA NGG 2 0.0 Tier II
22 NC_000073.6 7 101993060 + Numa1 NNNAAGCTTTCTGGTAGGGG NAG 2 0.0 Tier II
23 NC_000078.6 12 37940514 - Dgkb NNNAAGCTTTCTGTTTGGGG NGG 2 0.0 Tier II
24 NC_000073.6 7 138588223 - Gm30751 NNNAATCTTTCTGCTAGGGT NGG 2 0.4667 Tier III
25 NC_000079.6 13 23153536 - Vmn1r-ps129 NNNAAGCTTTCTCCAAGGGG NGG 2 0.2438 Tier III
26 NC_000085.6 19 38316401 - Gm9886 NNNAAGCTTTCTGCTGGGAG NGG 2 0.1374 Tier III
27 NC_000081.6 15 87127581 - A930027H12Rik NNNATGCTTTCTGCTGGGGG NGG 2 0.0699 Tier III
28 NC_000075.6 9 21154029 - Gm35977 NNNAAGCTTTCTGCTCTGGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)